Acute intestinal anisakiasis in Spain: a fourth-stage Anisakis simplex larva

Abstract

A case of acute intestinal anisakiasis has been reported; a nematode larva being found in the submucosa of the ileum of a woman in Jaén (Spain). The source of infection was the ingestion of raw MATERIALS AND METHODSWe have studied a new case of acute intestinal anisakiasis in Spain. On 28 November 1998, a 46-year-old Spanish woman, lifelong resident of Jaén, was admitted to the emergency room at the Hospital Ciudad de Jaén, where her clinical, radiological and analytical report was compatible with acute appendicitis. She had had during two days acute abdominal pain, diarrhea, meteorism, selective pain palpatation in the right ileum fossa, no nausea, vomit or fever and Blumberg sign negative. The laboratory data showed leukocytosis (13.780/mm 3 ), neutrophilia (80%) and 4% of eosinophils. The rest of the red series, white and biochemical, were normal. Plain radiographs of the abdomen and an electrocardiogram were within normal limits. The clinical diagnosis was acute appendicitis. After a McBurney incision, the appendix was found to be normal, with abundant intraperitoneal exudate and inflammatory induration located in the terminal ileum, which showed a microperforation. A prophylactic appendectomy was performed, followed by a wedge resection of the indurated intestinal zone. The layers of the abdominal wall were cleaned and closed. The indurated zone was opened and, in the submucosa of the terminal ileum, a nematode of 2.5 cm in length was found. The neighbouring tissues were analyzed, and the caecal appendix was found with submucosal sclerolipmatosis, and the inflamed ileum was infiltrated with abundant eosinophils.The removed larva, still moving, were placed in saline solution and fixed in 2.5% (v/v) buffered glutaraldehyde (pH 7.2), and examined first under an optical microscope and later processed and examinded under a scanning electron microscope.+ Corresponding

First record of anisakid juveniles (Nematoda) in the European seabass Dicentrarchus labrax (family: Moronidae), and their role as bio-indicators of heavy metal pollution

“…The incidence of which is increasing with the growing trend for consumption of raw, under-cooked seafood (Rosales et al 1999). Infection with anisakids can affect the commercial value of fish, particularly when larvae are located in the musculature and thus represent important economical loss for the fishing industry because their larvae may attempt to penetrate the gastrointestinal wall causing acute gastric or abdominal symptoms (Angot and Brasseur 1995).…”

Expression patterns and structural modelling of Hsp70 and Hsp90 in a fish-borne zoonotic nematode Anisakis pegreffii

“…L3 and L4 stages of Anisakis cause human health effects because fish maintain the L3 larvae that are infective to humans and marine mammals (Audicana et al, 2002), and the parasite can pass from the L3 to L4 stage in humans (Rosales et al, 1999). We found the mRNA and protein levels of ApHsp70 and ApHsp90 higher in L4 than L3 larvae.Differences in expression levels of Hsp70 and Hsp90 at different development stages may be due to the different regulatory systems (Yu et al, 2012).…”

Anisakid parasites of two forkbeards (Phycis blennoides and Phycis phycis) from the eastern Mediterranean coasts in Tunisia

“…The species H. aduncum was initially considered to be a possible etiologic agent of this disease. In Spain, only eight cases of anisakiasis have been published: one case of gastric anisakiasis, four cases of intestinal (two in the ileum, one in the appendix and one in the upper colon) and two of ectopic anisakiasis (larvae in the abdominal cavity associated with intestinal lesions) (Rosales et al 1999).…”