Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns

Barros, Julio César de Siqueira; Pinheiro, Sebastião R.S.; Bozza, Marcelo T.; Gueiros-Filho, Frederico José; Bello, Alexandre Ribeiro; Lopes, Ulisses Gazos; Pereira, José Augusto Adler

doi:10.1590/s0074-02761999000600016

Cited by 5 publications

(9 citation statements)

References 20 publications

(21 reference statements)

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“…Some other studies have shown that Amx would enrich the betalactam resistance genes and corresponding antibiotic resistant bacteria in the intestine [39,54]. Taking Gen would also promote the spread of antibiotic resistant bacteria and ARGs [15,16]. The above conclusions would help us infer the combined exposure effects on the increase of ARGs and human disease-related genes in this study.…”

Section: The Restoration Effects Of Probiotics and Synbiotics On Funcmentioning

confidence: 57%

“…Besides, gentamicin (Gen) is known as an aminoglycoside drug that binds to the 30S subunit of the ribosome to block bacterial protein synthesis [14]. Over the last several years, the effects of Amx or Gen on the human intestinal microbiota have been extensively studied [15][16][17][18][19]. However, very few research papers have reported their combination effects on intestinal microbial ecosystem.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

Liu¹,

Qi²,

Suo³

et al. 2020

Preprint

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Background: Antibiotics are emerging toxic contaminant that have potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics on human intestinal microbiota dysbiosis and related health risk are not fully understood. Moreover, there is limited information on using probiotics or synbiotics for restoration of intestinal microbiome affected by antibiotics. Therefore, this study evaluated the in vitro ability of combined effects of amoxicillin (Amx) and gentamycin (Gen), and the restoration effects of probiotics or synbiotics on ARGs as well as human disease-related pathways in the simulated human gut.Results: This study indicated that the combination exposure of Amx and Gen was confirmed to promote the increase of most ARGs and the disease-related pathways, which may be better restored by probiotics treatment. The results of the alpha diversity of the combined antibiotics exposure or the recovery microbial community showed no difference from the control. However, the beta diversity results indicated their differences, and the ascending colon sample recovered better under natural condition while the descending colon sample recovered better after probiotics treatment. Combination effects on the genetic level might attribute to microbiota shift, which were explained well by the phenomenon that Escherichia/Shigella was positively associated with the ARGs, and Klebsiella and Escherichia/Shigella were positively related to the human disease-related pathways.Conclusion: These results might be valuable to direct the future work and opened up new perspectives to address the direct effects of combine antibiotics on the intestinal microbiota and find a promising strategy to restore the antibiotics associated dysbiosis of gut microbiota.

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Section: The Restoration Effects Of Probiotics and Synbiotics On Funcmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

Liu¹,

Qi²,

Suo³

et al. 2020

Preprint

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“…Immunosuppression, stress, administration of antibiotics or antacids play an important role in altering defense mechanisms, allowing microorganisms present in food to replace the normal gastrointestinal £ora. Additionally, the intestinal environment is a scenario of selective processes due to therapeutic and prophylactic use of antimicrobial agents representing an imperceptible reservoir of multiresistant bacteria [3]. Additionally, the intestinal environment is a scenario of selective processes due to therapeutic and prophylactic use of antimicrobial agents representing an imperceptible reservoir of multiresistant bacteria [3].…”

Section: Introductionmentioning

confidence: 99%

“…The importance of the colonization of skin and mucosa has been recognized as a crucial step in the development of nosocomial infections [2]. Additionally, the intestinal environment is a scenario of selective processes due to therapeutic and prophylactic use of antimicrobial agents representing an imperceptible reservoir of multiresistant bacteria [3].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance in Gram-negative bacilli isolated from infant formulas

Carneiro

Silva

Merquior

et al. 2003

FEMS Microbiology Letters

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A total of 90 samples of infant formula (IF) were collected from the lactary of a teaching hospital, during a 4-month period from July to August 1999. The sanitary conditions of the formulas were analyzed, and a physiological characterization of Gram-negative bacillus isolates and antimicrobial susceptibility testing were performed. Colony counts were considered to be unacceptable for the majority of the IF samples and the contamination rates were related to inadequate handling. Coliforms (35 degrees C and 45 degrees C growth) were detected in most of the IF tested. Klebsiella pneumoniae, Citrobacter freundii, Cedacea davisae, Klebsiella planticola and Enterobacter cloacae were the isolates most commonly identified. Antimicrobial susceptibility testing showed significant resistance rates, particularly to amoxicillin/clavulanic acid, cefoxitin, cephalotin or ampicillin. One extended-spectrum beta-lactamase-producing K. pneumoniae strain was also recovered.

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“…In a previous study with Enterobacteriaceae strains of hospital origin we searched for genetic elements which express multidrug-resistance including resistance to aminoglycosides (10). Probes were built to detect plasmids encoding these multidrugresistance traits.…”

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confidence: 99%

Detección y caracterización microbiológica de enterobacterias multidrogorresistentes transportadoras del gen modificador de aminoglicósidos durante tres décadas en un hospital universitario de Río de Janeiro

et al. 2014

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Introduction: Multidrug-resistant Enterobacteriaceae, particularly those resistant to gentamicin, have become one of the most important causes of nosocomial infections. Objective: We sought to investigate the presence of genes conferring resistance to aminoglycosides, specially to gentamicin, in Klebsiella pneumoniae and Escherichia coli multidrug-resistant strains isolated from different clinical materials among patients hospitalized in a university hospital in Rio de Janeiro, Brazil. Materials and methods:Ten colonization strains and 20 infection strains were evaluated during three decades (1980 to 2010) using selective media containing 8 µg/ml of gentamicin. Thirty strains were tested for antimicrobial susceptibility. Twenty two strains were subjected to plasmid DNA extraction and 12 to hybridization assays using as probe a 1.9 kb plasmid DNA fragment from one of the K. pneumoniae strains isolated from faecal samples. This fragment was sequenced and assigned to the GQ422439 GenBank record. PCR was also performed using oligonucleotides designed for aminoglycosidemodifying enzymes. Results: An accC2 acetylase, besides transposons and insertion sequences, were evidenced. Twentyfour (80%) of the isolates were positive for the aacC2 gene in agreement with antibiotic susceptibility testing profiles, indicating the persistent presence of this gene throughout the three decades. We detected high molecular weight plasmids in 54,5% of the strains. Of the tested strains, 91% showed positive signal in the hybridization assays. Conclusion: A gene codifying for one specific aminoglycoside-modifying enzyme was detected all throughout the three decades. Our data back the adoption of preventive measures, such as a more conscious use of antimicrobial agents in hospital environments, which can contribute to control the dissemination of microorganisms harboring resistance gene plasmids.

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Evidences of gentamicin resistance amplification in Klebsiella pneumoniae isolated from faeces of hospitalized newborns

Cited by 5 publications

References 20 publications

Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

Antimicrobial resistance in Gram-negative bacilli isolated from infant formulas

Detección y caracterización microbiológica de enterobacterias multidrogorresistentes transportadoras del gen modificador de aminoglicósidos durante tres décadas en un hospital universitario de Río de Janeiro

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