A new strain of Bacillus thuringiensis Serovar israelensis very active against blackfly larvae

Rabinovitch, Leon; Cavados, Clara de Fátima Gomes; Chaves, Jeane Q.; Coutinho, Carlos José P. C. A.; Zahner, Viviane; Silva, Katia Regina A; Seldin, Ludy

doi:10.1590/s0074-02761999000500024

Cited by 8 publications

(7 citation statements)

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“…aegypti larvae, but which was more toxic than this strain when the bioassays were performed against Chrysomya megacephala (Diptera, Calliphoridae) and it is necessary to say that this strain does not produce β-exotoxin. Rabinovitch et al (1999) (Lecadet et al 1999). It is interesting that seven of the "autoagglutinating" strains isolated had the same protein profile of the B. thuringiensis serovar israelensis strain IPS-82, the same results described by Ragni et al (1996).…”

Section: Discussionsupporting

confidence: 60%

Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae and adults

Cavados¹,

Fonseca²,

Chaves³

et al. 2001

Mem. Inst. Oswaldo Cruz

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Entomopathogenic bacteria isolated fromSimuliids are hematophagus insects naturally occurring in waterways throughout Brazil. Although some species have been involved in the transmission of human onchocerciasis in other regions (Gerais & Ribeiro 1986, Maia-Herzog et al. 1999) the main importance of these insects in the southeast of Brazil is socio-economic. The frequent and intensive attacks by simuliids on the transitory population in this area can reduce tourism in summer months, a fact that could negatively impact the economies of affected municipalities. To decrease the presence of these insects in these areas, a full-scale programme, based on Bacillus thuringiensis serovar israelensis, has been carried out in the north littoral zone of the State of São Paulo since 1990 (Araújo-Coutinho 1995).Recent findings of B. thuringiensis serovar oswaldocruzi and B. thuringiensis serovar braziliensis from unusual habitats (Rabinovitch et al. 1995) encourage the continued search for other endemic entomopathogens. For that purpose, Simulium larvae and adults from the north littoral zone of São Paulo and some other rivers in the State of Rio de Janeiro were evaluated for the presence of entomopathogenic bacteria. MATERIALS AND METHODSCollection of simuliids -Black fly larvae and adults were collected from breeding sites in Pau d'Alho river, State of São Paulo. This river was divided into two regions, one downstream of B. thuringiensis serovar israelensis applications and another, upstream. Another collection point was the Soberbo river, Guapimirim, State of Rio de Janeiro (Fonseca et al. 1998). This river has never been exposed to commercial formulations based on B. thuringiensis serovar israelensis. Only larvae were collected in this waterway. The third point where larvae were collected was in Rio das Pedras, Mangaratiba, State of Rio de Janeiro.Isolation of Bacillus -To eliminate external contamination, the insects were sterilized following the methodology described by Alves (1986) with a slight modification; "Superbonder" glue (cyanoacrilate ester) was used to close both oral and anal cavities. This modification was adopted because Simulium larvae are very small and the dental floss used in the original technique was not applicable. After this step, the original method was performed passing the larvae through three solutions, first in 70% alcohol for 2 sec, second in 5% sodium hypoclorite for 3 min and finally in sterile 10% sodium thiossulfate for 5 min. The specimens were then washed three times in sterile distilled water.Two different methods were used to isolate B. thuringiensis samples. In both, Simulium larvae were transferred aseptically into a sterile mortar and macerated with a sterile pestle. In the first method, the macerate was placed in tubes containing distilled water (10 ml). The suspension was heated (65ºC) for 12 min, and immediately diluted (1x10 -2

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Section: Discussionsupporting

confidence: 60%

Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae and adults

Cavados¹,

Fonseca²,

Chaves³

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…This diversity makes comparison of results difficult. Rabinovitch et al (1999) must be conducted to determine the formulation potency in international toxic units (ITU), as well as its efficacy and shelf life.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a new formulation of Bacillus thuringiensis israelensis

2010

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The aim of this study was to determine the potency (ITU) and efficacy of a liquid formulation of Bacillus thuringiensis israelensis developed by the State University of Londrina named BioUel, against early fourth instar larvae of Aedes aegypti and Culex quinquefasciatus. The ITU/mg of BioUel was 960, the LC50 was of 0.271 (± 0.39) ppm, and the LC95 was 0.634 (± 0.099) ppm, in larvae of C. quinquefasciatus. In A. aegypti larvae, LC50 was 0.332 (± 0.042) ppm and LC95 was 0.694 (± 0.073) ppm. The ITU level of BioUel and its control results were similar to most commercial products tested. Stability was of approximately 90 days, which allows for local production.

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“…However, under some circumstances, the Bt and B. cereus spores may germinate and develop successfully, such as in humid, nutrient-rich soils with pH levels that are close to neutral, even when other microbial communities are present [ 38 ]. The rhizosphere colonization by B. cereus and Bt has rarely been reported [ 39 , 40 ]. A Bt strain that was isolated from the rhizosphere of Ficus doliaria proved to be very toxic to the Blackfly larvae that were absent in the region [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Bacillus thuringiensis and Bacillus cereus against Rhynchophorus ferrugineus

Elsharkawy

Almasoud²,

Alsulaiman³

et al. 2022

Insects

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The Red Palm Weevil (Rhynchophorus ferrugineus (Oliv.) (Coleoptera, Dryophthoridae) is a well-known palm tree pest that has caused enormous economic damage all over the globe. Insecticides are still the primary method of controlling this pest at this period. However, field populations of RPW have been shown to be resistant to pesticides. Using Bacillus spp. might be one of the options for controlling R. ferruginous. In this study, 23 species of Bacillus spp. were isolated from the rhizosphere of date palm trees in Al Ahsa Oasis, Saudi Arabia. The isolates were identified using 16S rRNA gene sequencing. R. ferrugineus larvae and adults were tested on sugarcane pieces that were treated with the B. thuringiensis strain PDC-AHSAA1 and B. cereus strains (PDC-AHSAA2, PDC-AHSA3 and PDC-AHSA4). The LC50 values for larvae and adults were quite low when they were compared with those of the other isolated strains. The B. thuringiensis strain PDC-AHSAA1 was more effective against both the larvae and adults. The determined LC50 values for B. thuringiensis ranged from 4.19 × 107 - 3.78 × 109. After 21 days, the data on larval mortality and body weight were evaluated. The surviving larvae that were treated with the bacterial isolates did not acquire a substantial weight. For the RPW larvae and adults, the mortality and corrected mortality death rates were increased by increasing the concentration of B. thuringiensis. In conclusion, Bacillus-treated diets negatively influenced the growth and development of the RPW. This research reported on the interaction between the RPW and the rhizosphere Bacillus spp. and highlighted the tremendous potential for the development of microbial resource-based control strategies for this pest.

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A new strain of Bacillus thuringiensis Serovar israelensis very active against blackfly larvae

Cited by 8 publications

References 0 publications

Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae and adults

Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae and adults

Evaluation of a new formulation of Bacillus thuringiensis israelensis

Efficiency of Bacillus thuringiensis and Bacillus cereus against Rhynchophorus ferrugineus

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