Influence of Growth Medium in Proteinase and Pigment Production by Streptomyces cyaneus

Petinate, Simone Dias Gonçalves; Martins, Rosana M; Coelho, R. R. R.; Meirelles, Maria de Nazareth Leal de; Branquinha, Marta H.; Vermelho, Alane Beatriz

doi:10.1590/s0074-02761999000200008

Cited by 16 publications

(9 citation statements)

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“…These results were synonymous to that have been investigated by (Nadeem et al, 2008). The results of the effect of incubation time confirmed the observation of which reported that an increase of protease production by Streptomyces cyanens and S. rimosus during log phase (Petinate et al, 1999;Yang and Wang, 1999). This indicates that high level of protease production is observed during active biomass production.…”

Section: Discussionsupporting

confidence: 87%

OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORM

Abdelwahed¹,

Danial

El‐Naggar³

et al. 2014

American Journal of Biochemistry and Biotechnology

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Section: Discussionsupporting

confidence: 87%

OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORM

Abdelwahed¹,

Danial

El‐Naggar³

et al. 2014

American Journal of Biochemistry and Biotechnology

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“…BHI medium in comparison to the others contains higher levels of peptones, proteins, and salts [15], which appear to facilitate planktonic growth and the biofilm yield of the two species. Protease peptone and infusions (calf brain and beef heart) provide the necessary nitrogen compounds, carbon, essential growth factors, amino acids, and vitamins.…”

Section: Discussionmentioning

confidence: 99%

Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus

et al. 2018

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Objective: Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. Materials and Methods: We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and scanning electron microscopy (SEM). Results: The most robust growth of the mono- and dual-species cultures was noted in BHI broth. On the contrary, RPMI 1640 medium promoted maximal initial adhesion of both the mono- and dual-species, but BHI broth fostered the maximal biofilm growth. SEM images showed profuse extracellular polysaccharide production in biofilms, particularly in coculture, in BHI medium. Conclusion: Our data demonstrate that BHI broth, relative to the other tested media, is the most conducive for in vitro evaluation of biofilm and planktonic growth kinetics of these two pathogens, both in mono- and coculture.

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“…Several microbial species are reported to produce alkaline protease. The most extensively studied alkaline protease producing bacteria are the genera of Bacillus (Kalisz 1998;Gupta et al, 2002), Pseudomonas (Bayoudh et al,2000) and actinomycetesStreptomyces species (Petinate et al, 1999), among Fungi the genera of Conidiobolus (Bhosale et al, 1995), Rhizopus (Banerjee and Bhattacharyya, 1992) and Aspergillus, and among yeasts is the genus Candida. Various solid substrates, such as wheat bran, soybean meal etc.…”

Section: Alkaline Proteasesmentioning

confidence: 99%

Production of alkaline protease from Aspergillus oryzae isolated from seashore of Bay of Bengal

Yepuru

2018

JANS

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Aspergillus oryzae isolatedon Potato dextrose agar from soil samples of kottakoduru seashore of Bay of Bengal, Andhra Pradesh, India seashore of Bay of Bengal by spread plate method and was screened for alkaline protease production on Skim milk containing agar plates and identified by clear zones of protein hydrolysis around colonies. Different physical and chemical parameters such as pH, temperature, substrate concentration and incubation time were optimized for the better production of alkaline protease. The maximum protease activity was found at pH of 8 containing 10% wheat bran at 300C, after 72 hours of fermentation.ZnSO4was effective activator for protease activity and sodium dsulphate had shownmore than 50% inhibition of enzyme activity. Among the different oil cakes used for the production of enzyme the Sesame oil cake proved to be suitable substrate after wheat bran for the production of protease by Aspergillus oryzae.

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Influence of Growth Medium in Proteinase and Pigment Production by Streptomyces cyaneus

Cited by 16 publications

References 0 publications

OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY <i>STREPTOMYCES AMBOFACIENS</i> IN FREE AND IMMOBILIZED FORM

OPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY <i>STREPTOMYCES AMBOFACIENS</i> IN FREE AND IMMOBILIZED FORM

Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of <b><i>Pseudomonas aeruginosa</i></b> and <b><i>Staphylococcus aureus</i></b>

Production of alkaline protease from Aspergillus oryzae isolated from seashore of Bay of Bengal

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