Evidence for functional ryanodine receptors in Schistosoma mansoni and their putative role in the control of calcium homeostasis

Silva, C. L. M.; Mendonça-Silva, Dayde Lane; Noël, François

doi:10.1590/s0074-02761998000700051

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ADPR, at least in mammalian cells, is known to regulate Ca 2+ influx across the plasma membrane by binding to the nudix motif on the transient receptor potential channel TRPM2 (32)(33)(34), while NAADP + mediates intracellular Ca 2+ release through RyRs in mammalian cells (27)(28)(29)(30)(31). Interestingly, smooth muscle fibers isolated from disassociated adult S. mansoni worms express RyRs that release Ca 2+ in response to ryanodine (75,76) and undergo contraction in response to RyR agonists such as caffeine (77). Schistosomes are also known to express voltage-gated calcium channels (78) and express a cDNA with weak similarity to human TRPC3 (TIGR S. mansoni GenBank Accession No.…”

Section: Discussionmentioning

confidence: 99%

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni^,

et al. 2005

View full text Add to dashboard Cite

ADP-ribosyl cyclases are structurally conserved enzymes that are best known for catalyzing the production of the calcium-mobilizing metabolite, cyclic adenosine diphosphate ribose (cADPR), from nicotinamide adenine dinucleotide (NAD(+)). However, these enzymes also produce adenosine diphosphate ribose (ADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP(+)), both of which have been shown to modulate calcium mobilization in vitro. We have now characterized a new member of the cyclase family from Schistosoma mansoni, a member of the Platyhelminthes phylum. We show that the novel NAD(P)(+) catabolizing enzyme (NACE) expressed by schistosomes is structurally most closely related to the cyclases cloned from Aplysia but also shows significant homology with the mammalian cyclases, CD38 and CD157. NACE expression is developmentally regulated in schistosomes, and the GPI-anchored protein is localized to the outer tegument of the adult schistosome. Importantly, NACE, like all members of the cyclase family, is a multifunctional enzyme and catalyzes NAD(+) glycohydrolase and base-exchange reactions to produce ADPR and NAADP(+). However, despite being competent to generate a cyclic product from NGD(+), a nonphysiologic surrogate substrate, NACE is so far the only enzyme in the cyclase family that is unable to produce significant amounts of cADPR (<0.02% of reaction products) using NAD(+) as the substrate. This suggests that the other calcium-mobilizing metabolites produced by NACE may be more important for calcium signaling in schistosomes. Alternatively, the function of NACE may be to catabolize extracellular NAD(+) to prevent its use by host enzymes that utilize this source of NAD(+) to facilitate immune responses.

show abstract

Section: Discussionmentioning

confidence: 99%

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni^,

et al. 2005

View full text Add to dashboard Cite

show abstract

Pharmacological characterisation of neuropeptide F (NPF)-induced effects on the motility of Mesocestoides corti (syn. Mesocestoides vogae) larvae

Hrčková

Velebný

Halton

et al. 2004

International Journal for Parasitology

View full text Add to dashboard Cite

Identification of functional FKB protein in Echinococcus granulosus: Its involvement in the protoscolicidal action of rapamycin derivates and in calcium homeostasis

Cumino

Lamenza

Denegri

2010

International Journal for Parasitology

View full text Add to dashboard Cite

Evidence for functional ryanodine receptors in Schistosoma mansoni and their putative role in the control of calcium homeostasis

Cited by 2 publications

References 0 publications

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni^,

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni^,

Pharmacological characterisation of neuropeptide F (NPF)-induced effects on the motility of Mesocestoides corti (syn. Mesocestoides vogae) larvae

Identification of functional FKB protein in Echinococcus granulosus: Its involvement in the protoscolicidal action of rapamycin derivates and in calcium homeostasis

Contact Info

Product

Resources

About

Evidence for functional ryanodine receptors in Schistosoma mansoni and their putative role in the control of calcium homeostasis

Cited by 2 publications

References 0 publications

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni,

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni,

Pharmacological characterisation of neuropeptide F (NPF)-induced effects on the motility of Mesocestoides corti (syn. Mesocestoides vogae) larvae

Identification of functional FKB protein in Echinococcus granulosus: Its involvement in the protoscolicidal action of rapamycin derivates and in calcium homeostasis

Contact Info

Product

Resources

About

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni^,

Production of Calcium-Mobilizing Metabolites by a Novel Member of the ADP-Ribosyl Cyclase Family Expressed inSchistosoma mansoni^,