Transcription levels of two actin genes (SmAct and SmAct2), cytochrome C oxidase subunit II (SmCOXII), triosephosphate ssomerase (TPI), and a putative translation regulatory protein EIF-5 during the first seven days of in vitro development of Schistosoma mansoni schistosomula

Oliveira, Guilherme; Busek, Solange U.; Corrêa-Oliveira, Rodrigo

doi:10.1590/s0074-02761998000700038

Cited by 5 publications

(3 citation statements)

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“…Reactions were carried out in a final volume of 25 µl in 96 well plates. S. mansoni cytochrome C oxidase I (GenBank AF216698) was used as the sample normalizing transcript [28] , [29] , as it has been shown to be highly and constitutively expressed in various S. mansoni life-cycle stages [30] , [27] and GFP cDNA was used as endogenous control [31] . Two internal controls assessing both possible genomic DNA contaminations (no reverse transcriptase) and purity of the reagents (no cDNA) were included.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway

et al. 2014

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BackgroundProtein kinases are proven targets for drug development with an increasing number of eukaryotic Protein Kinase (ePK) inhibitors now approved as drugs. Mitogen-activated protein kinase (MAPK) family members connect cell-surface receptors to regulatory targets within cells and influence a number of tissue-specific biological activities such as cell proliferation, differentiation and survival. However, the contributions of members of the MAPK pathway to schistosome development and survival are unclear.Methodology/Principal FindingsWe employed RNA interference (RNAi) to elucidate the functional roles of five S. mansoni genes (SmCaMK2, SmJNK, SmERK1, SmERK2 and SmRas) involved in MAPK signaling pathway. Mice were injected with post-infective larvae (schistosomula) subsequent to RNAi and the development of adult worms observed. The data demonstrate that SmJNK participates in parasite maturation and survival of the parasites, whereas SmERK are involved in egg production as infected mice had significantly lower egg burdens with female worms presenting underdeveloped ovaries. Furthermore, it was shown that the c-fos transcription factor was overexpressed in parasites submitted to RNAi of SmERK1, SmJNK and SmCaMK2 indicating its putative involvement in gene regulation in this parasite's MAPK signaling cascade.ConclusionsWe conclude that MAPKs proteins play important roles in the parasite in vivo survival, being essential for normal development and successful survival and reproduction of the schistosome parasite. Moreover SmERK and SmJNK are potential targets for drug development.

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Section: Methodsmentioning

confidence: 99%

Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway

et al. 2014

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“…A One-Step RT-PCR kit (QIAGEN) was used and the protocol followed according to the manufacturer's instructions. Primer pairs were designed against the two S. japonicum ferritins (GenBank accessions given earlier) and schistosome triphosphate isomerase (TPI) (see Oliveira, Busek, & Correa-Oliveira, 1998 ). For these experiments the primers sets amplified transcripts of approximately 500 bp ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Tracking the fate of iron in early development of human blood flukes

Jones

McManus

Sivadorai

et al. 2007

The International Journal of Biochemistry & Cell Biology

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Iron (Fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. One role for Fe in some invertebrates is in stabilization of extracellular matrices. The human blood fluke, Schistosoma japonicum, is responsible for significant human disease in developing and tropical nations. Disease in humans arises from host immunological reaction to parasite eggs that lodge in tissues. Schistosomes require Fe for development in their hosts, and store abundant Fe in vitelline (eggshell-forming) cells of the female system. The understanding of Fe metabolism and functionality are aspects of its biology that may be exploited in future therapeutics. The biology of Fe stores in vitelline cells of S. japonicum was investigated to illuminate possible functions of this element in early development of these parasites. Vitelline Fe is stored in yolk ferritin that is upregulated in females and is also expressed at low levels in egg-stages and adult males. Laser microdissection microscopy, coupled with reverse transcriptase- and real time-PCR amplification of schistosome ferritin sequences, confirmed that the vitelline cells are the likely progenitor cells of yolk ferritin. Assessment of Fe concentrations in whole male and whole female adult worms, eggs and purified eggshells by colorimetric assays and mass spectroscopy demonstrated higher levels of Fe in the female parasite, but also high levels of the element in whole parasite eggs and purified eggshell. Qualitative energy dispersive spectroscopy of purified eggshells, revealed that Fe is abundant in the eggshell, the matrix of which is composed of heavily cross-linked eggshell precursor proteins. Thus, vitelline stores of Fe are implicated in eggshell cross-linking in platyhelminths. These observations emphasise the importance of Fe in schistosome metabolism and egg formation and suggest new avenues for disruption of egg formation in these pathogenic parasites.

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“…In these assays, a-tubulin (Duvaux-Miret et al 1991) was used as an internal standard (Mei and Loverde 1997) but our results showing that b-tubulin could be differentially expressed in sporocysts led us to consider an additional standard, the cytochrome c oxidase subunit II (SmCOXII), a mitochondrial enzyme shown to be transcriptionally stable in schistosomula (Oliveira et al 1998). Results in Fig.…”

Section: Confirmation Of Differential Displaysmentioning

confidence: 99%

Gene expression changes in Schistosoma mansoni sporocysts induced by Biomphalaria glabrata embryonic cells

Coppin

Lefebvre

Caby

et al. 2003

Parasitology Research

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Biomphalaria glabrataembryonic (Bge) cells have been shown to provide favourable environmental conditions for the development of Schistosoma mansoni sporocysts. We investigated the effect of Bge excretory-secretory products on metabolic activity and gene transcription in S. mansoni mother sporocysts. Using the differential-display technique, we identified several sporocyst transcripts regulated by exposure to Bge soluble components. Research in databases indicated that six of the eight differential products analysed were homologous to sequences already present in databases. Two transcripts appeared of interest for schistosome development since they could be associated with cell division and protein synthesis in developing sporocysts. Their up-regulation following contact with cell products was confirmed by semi-quantitative RT-PCR. The first fragment coded for a part of the chaperonin containing T-complex protein gamma subunit-like protein of S. mansoni (SmTCP 1-C). The second one represented a new S. mansoni expressed sequence tag encoding a protein homologous to various glutaminyl-tRNA synthetases (GlnRS). The full-length sequence of SmGlnRS was cloned from adult schistosomes and its primary sequence was compared to other GlnRS. The overexpression of SmTCP-1 and SmGlnRS could be correlated with the metabolic changes observed in Bge-exposed sporocysts.

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Transcription levels of two actin genes (SmAct and SmAct2), cytochrome C oxidase subunit II (SmCOXII), triosephosphate ssomerase (TPI), and a putative translation regulatory protein EIF-5 during the first seven days of in vitro development of Schistosoma mansoni schistosomula

Cited by 5 publications

References 0 publications

Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway

Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway

Tracking the fate of iron in early development of human blood flukes

Gene expression changes in Schistosoma mansoni sporocysts induced by Biomphalaria glabrata embryonic cells

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