Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

Oliveira, Diana Magalhães de; Carmo, Silvânia Antunes do; Silva-Teixeira, David Nascimento; Góes, Alfredo M.

doi:10.1590/s0074-02761998000700028

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…NO induces a modulatory effect upon in vitro and in vivo granuloma formation. This finding suggests a protective effect related to immune response modulation [32,33]. Our data are consistent with the literature showing increased production of TNF-α after stimulation with SWAP in the supernatant of cultures from the XTO group (Figure 3A and 3F).…”

Section: Discussionsupporting

confidence: 93%

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

et al. 2012

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BackgroundThe immune response to Schistosoma mansoni is characterized by a granulomatous reaction around the parasite eggs that are trapped in the host liver, and this reaction modulates the immune response during the chronic phase of the disease. The typical peripheral blood mononuclear cell (PBMC) response of patients during the chronic intestinal phase of infection is characterized by a decreased response to an S. mansoni soluble egg antigen. To obtain a greater understanding of Schistosoma infections, this study investigated the effects of the soluble egg antigen (SEA) and soluble adult worm antigen (SWAP) of S. mansoni on cellular proliferation, cytokine production, and ERK1/2 and Akt phosphorylation in PBMCs from infected (XTO) and egg-negative (NI) individuals living in the same endemic area.MethodsThe activation status was evaluated by cell immunophenotypic staining (cytometry). The cell proliferation assay was by CFSE method. Cytokine detection assay (Th1 and Th2) was by Cytometric Bead and Array phosphorylation status was by ELISA.ResultsThe XTO, NI and BD (blood donor) individuals from an area not endemic for schistosomiasis were compared. The CD4+ T lymphocyte proliferation rate was lower in the XTO group, but not the NI group, after SEA stimulation compared to the BD group. The CD8+ T cell proliferation rate was lower in the XTO group in the unstimulated cultures and after both SEA and SWAP stimulation compared to the BD group. Cytokine analysis after either SEA or SWAP stimulation showed a balanced cytokine pattern in the XTO and NI groups. ERK1/2 and Akt phosphorylation were only marginally detected in all groups; however, a decrease in ERK 1/2 phosphorylation was observed in the SWAP-stimulated XTO group compared to both the NI and BD groups.ConclusionsThe data indicate that SEA-stimulated CD4+ T cells from infected patients have a lower proliferation rate than the same cells from the NI group. Furthermore, we observed that SWAP stimulation influences ERK1/2 phosphorylation in the XTO group.

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Section: Discussionsupporting

confidence: 93%

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

et al. 2012

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“…Apoptotic pathways can be divided into caspase-dependent and caspase-independent cell death pathways, the latter showing a major involvement of ROS (Fiers et al 1999). It is also known that in schistosomiasis the host reaction involves the generation of ROS and RNS Gharib et al 1999;Kazura et al 1981Kazura et al , 1985McCormick et al 1996;Oliveira et al 1998Oliveira et al , 1999Pascal et al 2000). Depending on the level of oxidative stress, cellular proliferation, apoptosis or necrosis is induced (Toyokuni 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Studies in mice Gharib et al 1999;Kazura et al 1985;McCormick et al 1996;Oliveira et al 1998;) and humans (Kazura et al 1981;Oliveira et al 1999;Pascal et al 2000) have indicated that the host reaction involves the generation of free radicals. Free radicals, including reactive oxygen (ROS) and nitrogen species (RNS), are defined as any chemical species capable of independent existence that contains at least one unpaired electron (Toyokuni 1999).…”

Section: Introductionmentioning

confidence: 99%

“…In vitro experiments have indicated that nitric oxide (NO · ) is implicated in the immune host defence in schistosome-infected mice and humans (Oliveira et al 1998(Oliveira et al , 1999. NO · is a major effector molecule of macrophage cytotoxicity against a variety of microbial agents, including protozoan and helminth parasites (Oswald et al 1994).…”

Section: Introductionmentioning

confidence: 99%

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Role of reactive nitrogen species in neuronal cell damage during intestinal schistosomiasis

Nassauw

Bogers

Marck

et al. 2001

Cell and Tissue Research

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Free radicals are known to be involved in the host reaction during Schistosoma mansoni-induced inflammation in the liver and the intestine. In the present study, the influence of reactive nitrogen species (RNS) on the enteric neurons of infected ileum of mice was investigated. Cryosections and whole-mounts of the ileum of control, and 8- and 15-week-infected mice were processed for immunohistochemical localization of 3-nitrotyrosine, a biomarker of RNS, and of active caspase-3, a key executioner of apoptosis. An antibody directed against protein gene product 9.5 or S100 protein was used as a marker for neurons or enteroglial cells. In infected mice, but not in control animals, 3-nitrotyrosine was detected in parasite eggs and, as revealed by double immunolabelling, in some neuronal and enteroglial cells. Quantitative analysis of whole-mounts showed that the percentage of 3-nitrotyrosine-immunoreactive neurons significantly increased with time in both the submucous and myenteric plexus. Caspase-3 immunoreactivity was predominantly found in parasite eggs in infected mice. Immunoreactive enteric neurons were occasionally observed. The results indicate that inflammation-induced RNS are present in the ileum of S. mansoni-infected mice, and participate in the elimination of the schistosome eggs causing damage in a significant number of enteric neurons. However, neuronal cell death appears to be a rare phenomenon in the schistosome-infected mouse ileum.

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Parasite structure of the Ocean Whitefish Caulolatilus princeps from Baja California, México (East Pacific)

Rodríguez-Santiago¹,

Rosales‐Casián

2010

Helgol Mar Res

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The metazoan parasite fauna of Caulolatilus princeps from northern Baja California, Mexico is quantitatively described for the Wrst time. Further, the ecological aspects of prevalence, abundance, and intensity of infection are examined through an annual cycle. Six parasite species were recorded; 2 ectoparasites (1 monogenean and 1 copepod) and 4 endoparasites (2 digeneans and 2 nematodes). The digeneans Choanodera caulolatili and Bianium plicitum, the nematodes Anisakis sp. and Hysterothylacium sp., and the copepod Hatschekia sp. set new geographical and host records. The highest values of prevalence and abundance were in Anisakis sp. (prevalence = 93.3%, abundance = 12.4 § 4.7 ind/host) and in Hysterothylacium sp. (prevalence = 86.6%, abundance = 16.5 § 3.4 ind/host). The mean intensity of infection showed maximum values in summer (August = 14.2) and minimums in winter (February = 4.2). The mean intensity was higher in Hatschekia sp. (20.3 § 7.8) followed by Hysterothylacium sp. (18.6 § 1.4) and Anisakis sp. (12.9 § 2.2). Larval stages of Anisakis and Hysterothylacium were particularly important due to their high abundance and prevalence, because they represent a human health risk (anisakiasis). In addition, the relationships between the metazoan parasites of C. princeps and host size and weight, Wsh condition and water temperature (bottom) are discussed.

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Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

Abstract: Nitric oxide (NO) is an important effector molecule involved in immune regulation

Cited by 8 publications

References 27 publications

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

Role of reactive nitrogen species in neuronal cell damage during intestinal schistosomiasis

Parasite structure of the Ocean Whitefish Caulolatilus princeps from Baja California, México (East Pacific)

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