Molecular study of similar Biomphalaria species

Further studies on the molecular systematics of Biomphalaria snails from Brazil

“…In previous studies we demonstrated that restriction profiles resulting from digestion with DdeI permit the identification of B. glabrata, B. straminea and B. tenagophila (Vidigal et al 1998a,b). We also used this technique in the study of very similar species such as B. straminea, B. intermedia, B. kuhniana, B. peregrina ) and B. t. tenagophila, B. t. guaibensis and B. occidentalis (Spatz et al 1998(Spatz et al , 1999. The morphological separation of typical specimens of B. peregrina and B. oligoza are easy taste for the specialist in the identification of Biomphalaria snails.…”

“…This method was successfully used to construct the molecular key for Aedes species (West et al 1997). It was also used to distinguish closely related parasitic worms and others different organisms (Newton et al 1998, Wu et al 1999) and on studies of genetic variation and identification of snails of the genera Oncomelania, Bulinus and Biomphalaria (Hope & McManus 1994, Stothard et al 1996, Stothard & Rollinson 1997, Rollinson et al 1998, Vidigal et al 1998a,b, Caldeira et al 1998, Spatz et al 1998.…”

Small-Subunit Ribosomal Rna Gene Identification of the Human Pathogen Schistosoma Sp. In Egypt

“…It was also used to distinguish closely related parasitic worms and other different organisms (Wu et al, 1999) and. instudies of genetic variation and identification of snails of the genera Oncomelania, Bulinus and Biomphalaria (Hope & McManus 1994;Stothard et al, 1996;Stothard & Rollinson, 1997;Caldeira et al, 1998;Rollinson etal f 1998;Vidigal etaU 1998a,b andSpate e/a/., 1998;.…”

Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of internal transcriber spacer of the RNA ribosomal gene

“…Moreover, identification is also complicated by morphological similarities among some species of snail, for example the B. straminea (Paraense 1988 and B. tenagophila complexes (Spatz et al 1998(Spatz et al , 1999.…”

“…The polymerase chain reaction amplification and restriction fragment length polymorphism (PCR-RFLP) technique have been used successfully to distinguish several medically important snails of the genera Oncomelania (Hope & McManus 1994), Bulinus (Stothard et al 1996(Stothard et al , 1997 and Biomphalaria (Vidigal et al 1998a, b, Caldeira et al 1998, Spatz et al 1998, 1999. This technique is based on amplification of the internal transcribed spacer region of the rRNA followed by digestion of the resulting fragment with restriction enzymes.…”