Ultrastructural Observations of the Vitelline Cells of Metamicrocotyla macracantha (Monogenea, Microcotylidae)

Baptista-Farias, Maria de Fatima Diniz; Kohn, Anna

doi:10.1590/s0074-02761998000400023

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…This needs further clarification as there is no information regarding the vitelline ultrastructure in diplozoids. Other studies have documented similarities in ultrastructural development of vitelline cells between other monogeneans [ 51 , 52 ] and some digeneans [ 48 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Trace element and metal sequestration in vitellaria and sclerites, and reactive oxygen intermediates in a freshwater monogenean, Paradiplozoon ichthyoxanthon

Gilbert

Avenant‐Oldewage

2017

PLoS ONE

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Exposure to metals and other trace elements negatively affects infection dynamics of monogeneans, including diplozoids, but, physiological mechanisms linked to exposure have yet to be documented. In this study sequestration of trace elements and reactive oxygen intermediate production in the monogenean, Paradiplozoon ichthyoxanthon, was demonstrated. During dissection of host fish, Labeobarbus aeneus, the gills were excised and assessed for P. ichthyoxanthon, which were removed and frozen for fluorescence microscopy or fixed for transmission electron microscopy. Trace elements were sequestered in the vitellaria and sclerites in P. ichthyoxanthon, and the presence of reactive oxygen intermediates was observed predominantly in the tegument of the parasite. Trace elements and metals identified and ranked according to weight percentages (wt%) in the vitellaria were Cu > C > Au > O > Cr > Fe > Si while for the sclerites C > Cu > O > Au > Fe > Cr > Si were identified. For most element detected, readings were higher in the vitellaria than the sclerites, except for C and O which were higher in sclerites. Specifically for metals, all levels detected in the vitellaria were greater than in sclerites. Based on the proportion of trace elements present in the vitellaria and sclerites it appears that most trace elements including metals were sequestered in the vitellaria. The results of reactive oxygen intermediate production in the tegument of the parasite suggests either trace element accumulation takes place across the tegument or results from the action of the host’s immune response on the parasite. The results serve as the first demonstration of trace element sequestration and reactive oxygen intermediates in a freshwater monogenean parasite.

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Section: Discussionmentioning

confidence: 99%

Trace element and metal sequestration in vitellaria and sclerites, and reactive oxygen intermediates in a freshwater monogenean, Paradiplozoon ichthyoxanthon

Gilbert

Avenant‐Oldewage

2017

PLoS ONE

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“…The surface topography of M. macracantha was described through scanning electron microscopy by Kohn et al (1994), with their transmission electron microscopic observations related to those for spermiogenesis and spermatozoa as reported by Baptista-Farias et al (1995) and vitelline cells by Baptista-Farias & Kohn (1998).…”

Section: Introductionmentioning

confidence: 87%

Ultrastructure of the tegument of Metamicrocotyla macracantha (Alexander, 1954) Koratha, 1955 (Monogenea, Microcotylidae)

2004

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The ultrastructure of the body tegument of Metamicrocotyla macracantha (Alexander, 1954) Koratha, 1955, parasite of Mugil liza from Brazil, was studied by transmission electron microscopy. The body tegument is composed of an external syncytial layer, musculature, and an inner layer containing tegumental cells. The syncytium consists of a matrix containing three types of body inclusions and mitochondria. The musculature is constituted of several layers of longitudinal and circular muscle fibers. The tegumental cells present a well-developed nucleus, cytoplasm filled with ribosomes, rough endoplasmatic reticulum and mitochondria, and characteristic organelles of tegumental cells.

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“…According to Fried and Haseeb (1991), a review of the literature from 1975 to 1987 reveals that most authors follow the cleavage process for vitellogenesis of 4 stages for the Platyhelminthes. To date, the latter method has been used for Trematoda (Spence and Silk, 1971;Levron et al, 2010), Rhabditophora (Sopott-Ehlers, 1991, 1992Falleni et al, 1995Falleni et al, , 1998Falleni et al, , 2001Falleni et al, , 2002Falleni et al, , 2006, Eucestoda (Swiderski and Xylander, 2000;Swiderski et al, 2005), and Monogenea (Baptista-Farias and Kohn, 1998;Falleni et al, 2006). Nevertheless, several special features can be distinguished between the different species of Digenea.…”

Section: Folliclesmentioning

confidence: 99%

Ultrastructural Study of Vitellogenesis of Aphallus tubarium (Rudolphi, 1819) Poche, 1926 (Digenea: Cryptogonimidae), An Intestinal Parasite of Dentex dentex (Pisces: Teleostei)

Greani

Quilichini

Foata

et al. 2012

Journal of Parasitology

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Vitellogenesis of Aphallus tubarium, an intestinal parasite of Sparidae (Dentex dentex ), was studied by transmission electron microscopy. The ultrastructural features allowed us to distinguish 4 stages in the vitellogenesis process. In stage 1, vitellocytes have a cytoplasm mainly filled with ribosomes, but few mitochondria. In stage 2, there is an increase in amount of endoplasmic reticulum and few Golgi complexes. There is production of shell globules that coalesce into clusters in stage 3; some glycogen particles are observed via the Thiéry method. Finally, in stage 4, mature vitellocytes are filled with shell globule clusters and generally contain a large lipid droplet. Glycogen particles are grouped at the periphery of the cell.

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Ultrastructural Observations of the Vitelline Cells of Metamicrocotyla macracantha (Monogenea, Microcotylidae)

Abstract: An electron microscopic study of the vitelline follicles of Metamicrocotyla macracantha (Alexander, 1954) Koratha,1955

Cited by 9 publications

References 13 publications

Trace element and metal sequestration in vitellaria and sclerites, and reactive oxygen intermediates in a freshwater monogenean, Paradiplozoon ichthyoxanthon

Trace element and metal sequestration in vitellaria and sclerites, and reactive oxygen intermediates in a freshwater monogenean, Paradiplozoon ichthyoxanthon

Ultrastructure of the tegument of Metamicrocotyla macracantha (Alexander, 1954) Koratha, 1955 (Monogenea, Microcotylidae)

Ultrastructural Study of Vitellogenesis of Aphallus tubarium (Rudolphi, 1819) Poche, 1926 (Digenea: Cryptogonimidae), An Intestinal Parasite of Dentex dentex (Pisces: Teleostei)

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