Studies on the Bacillus sphaericus Larvicidal Activity against Malarial Vector Species in Amazonia

Rodrigues, Iléa Brandão; Tadei, Waderli Pedro; Dias, José Manoel C Silva

doi:10.1590/s0074-02761998000400005

Cited by 16 publications

(13 citation statements)

References 5 publications

(7 reference statements)

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“…The larvicidal activity of the ten Brazilian strain tested and evaluated by the association of LC 50 values. In this observation, the Anopheles nuneztovari with the three strains S 2 , S 20 , and S 46 were the most effective with LC 50 values between 0.084 and 0.127 ppm in 48 h reading (Rodrigues et al 1998). In present study, LC 90 0.01 mg/l of B. sphaericus was not found effective for larvae of (Rodrigues et al 1998;Poopathi and Tyagi 2002;Poopathi et al 1999;Rao et al 1995) (Table 2 and Figs.…”

Section: Resultsmentioning

confidence: 70%

Efficacy of Bacillus sphaericus against larvae of malaria and filarial vectors: an analysis of early resistance detection

Singh

Prakash

2008

Parasitol Res

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We are reporting in this paper the control of Anopheles stephensi Liston and Culex quinquefasciatus Say using Bacillus sphaericus. These have been now considered with a practical solution because of its specific and prolonged killing action against mosquito larvae. The efficacy of B. sphaericus were assessed against all instars of A. stephensi and C. quinquefasciatus separately. During the experiments, the mortalities were not found highly effective in dose concentration of LC(90) 0.01 mg/l. It is recommended by the World Health Organizations in all instars of larvae of A. stephensi and C. quinquefasciatus. Thereafter, six different concentrations were used in laboratory bioassays (05, 10, 20, 30, 40, and 50 mg/l) for A. stephensi. Similarly, in the case of C. quinquefasciatus, six statistically significant different concentrations were used (0.01, 0.04, 0.05, 0.10, 5.0, and 10.0 mg/l) of B. sphaericus. It was recorded after exposure of 24 h. The percentages of mortalities were different for the different instars of C. quinquefasciatus and A. stephensi. The probit equations were drawn by the probit method. The result of the study distinctly showed that B. sphaericus is not effective on selected defined doses against A. stephensi and C. quinquefasciatus. This probably indicates an initiation of resistance. This efficacy study can be useful while detecting early resistance phenomena in environment specific zones.

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Section: Resultsmentioning

confidence: 70%

Efficacy of Bacillus sphaericus against larvae of malaria and filarial vectors: an analysis of early resistance detection

Singh

Prakash

2008

Parasitol Res

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“…It was recorded after exposure of 24 h. The percentages of mortalities were different for the different instars of C. quinquefasciatus and A. stephensi. Bioassay studies of B. sphaericus have been carried out in different parts of the world, including India, on mosquitoes in laboratories and fields (Rodrigues et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Studies on larvicidal and pupicidal activity of Leucas aspera Willd. (Lamiaceae) and bacterial insecticide, Bacillus sphaericus, against malarial vector, Anopheles stephensi Liston. (Diptera: Culicidae)

et al. 2011

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The efficacy of whole plant extracts of Leucas aspera and Bacillus sphaericus has been proven against larvicidal and pupicidal activities of the malarial vector, Anopheles stephensi. The present study investigated the larvicidal and pupicidal activity against the first to fourth instar lavae and pupae of the laboratory-reared mosquitoes, A. stephensi. The medicinal plants were collected from the area around Maruthamalai hills, Coimbatore, Tamil Nadu, India. L. aspera whole plant was washed with tap water and shade dried at room temperature. The dried plant materials were powdered by an electric blender. From the powder, 100 g of the plant materials was extracted with 300 ml of organic solvents of ethanol for 8 h using a Soxhlet apparatus. The extracts were filtered through a Buchner funnel with Whatman number 1 filter paper. The crude plant extracts were evaporated to dryness in a rotary vacuum evaporator. The plant extract showed larvicidal and pupicidal effects after 24 h of exposure. All larval instars and pupae have considerably moderate mortality; however, the highest larval mortality was the ethanolic extract of whole plant L. aspera against the first to fourth instar larvae and pupae values of LC(50) = I instar was 9.695%, II instar was 10.272%, III instar was 10.823%, and IV instar was 11.303%, and pupae was 12.732%. B. spaericus against the first to fouth instar larvae and pupae had the following values: I instar was 0.051%, II instar was 0.057%, III instar was 0.062%, IV instar was 0.066%, and for the pupae was 0.073%. No mortality was observed in the control. The present results suggest that the ethanolic extracts of L. aspera and B. sphaericus provided an excellent potential for controlling of malarial vector, A. stephensi.

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“…In South America for example, considerable efforts are being made in testing new local bacterial strains, their formulations [106,107,108] and the possibility of combining such approaches with others that target mosquitoes at the adult stages [109,110,111,112,113]. Although only few studies were done to test the effect of Bti/Bs on African malaria vectors [38,39,40,41,42,43,44], and although these studies were more of experimental rather than large-scale practical application [45,46], their established results showed effective roles of these Bacillus strains, but highlighted the need for additional work at this particular level, along with broader disseminations and practical implications [105].…”

Section: Means Of Biological Controlmentioning

confidence: 99%

The Biological Control of the Malaria Vector

Kamareddine

2012

Toxins

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The call for malaria control, over the last century, marked a new epoch in the history of this disease. Many control strategies targeting either the Plasmodium parasite or the Anopheles vector were shown to be effective. Yet, the emergence of drug resistant parasites and insecticide resistant mosquito strains, along with numerous health, environmental, and ecological side effects of many chemical agents, highlighted the need to develop alternative tools that either complement or substitute conventional malaria control approaches. The use of biological means is considered a fundamental part of the recently launched malaria eradication program and has so far shown promising results, although this approach is still in its infancy. This review presents an overview of the most promising biological control tools for malaria eradication, namely fungi, bacteria, larvivorous fish, parasites, viruses and nematodes.

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Studies on the Bacillus sphaericus Larvicidal Activity against Malarial Vector Species in Amazonia

Abstract: In this work, bioassays were carried out in laboratory conditions (average temperature 26 ± 2ºC)

Cited by 16 publications

References 5 publications

Efficacy of Bacillus sphaericus against larvae of malaria and filarial vectors: an analysis of early resistance detection

Efficacy of Bacillus sphaericus against larvae of malaria and filarial vectors: an analysis of early resistance detection

Studies on larvicidal and pupicidal activity of Leucas aspera Willd. (Lamiaceae) and bacterial insecticide, Bacillus sphaericus, against malarial vector, Anopheles stephensi Liston. (Diptera: Culicidae)

The Biological Control of the Malaria Vector

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