An Improved Technique for the Dissection of Female Genitalia of Phlebotomine Sandflies (Diptera: Psychodidae), with an Improvement in the Handling of Insects

Marcondes, Carlos Brisóla

doi:10.1590/s0074-02761998000100019

Cited by 5 publications

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“…Collection chambers of traps were replaced at two hour intervals, the change being completed within 2-3 min. Sandflies were prepared for identification by NC method 34 .…”

Section: Methodsmentioning

confidence: 99%

Ecology of Phlebotomine sandflies (Diptera, Psychodidae) in Brazilian Atlantic Forest

Marcondes

Santos-Neto

Lozovei

2001

Rev. Soc. Bras. Med. Trop.

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Abstract Abstract Abstract The phlebotomine sandfly fauna of a primary forest reserve at Morretes (eastern Paraná State) was studied, using CDC-like light traps, one night per month, at canopy and ground level, between April 1995 and March 1996. A total of 3,106 insects were collected, identified as belonging to nine species. Lutzomyia ayrozai and Lu. geniculata were predominant, seven other species also being present. Monthly mean temperature, rainfall and the temperature of the collection night significantly influenced the numbers of Lu. ayrozai while the two first factors influenced the numbers of Lu. geniculata, besides the collected quantities of females of the two species. The influence of the factors on Lu. ayrozai numbers was more immediate than in those of Lu. geniculata. Numbers of both species and of the females of Lu. geniculata collected in different seasons, but not at the different heights, varied significantly. Differences between the behaviour of Lu. ayrozai in Morretes and in other regions could be attributed to environmental differences and/or to regional variations in the species, which could constitute species complexes. Hourly variations of collections were different in the species and seasons. Key-words: Key-words: Key-words: Key-words: Key-words: Phlebotominae. Fauna. Seasonal variation. Psychodidae. Height influence. Lutzomyia. Resumo Resumo ResumoResumo Resumo A fauna de flebotomíneos de uma reserva de floresta primária em Morretes (leste do Estado do Paraná) foi estudada, utilizando armadilhas luminosas tipo CDC, uma noite por mês, no nível do solo e da copa, entre abril de 1995 e março de 1996. Um total de 3.106 insetos foi coletado. Lu. ayrozai e Lu. geniculata foram predominantes, além de outras sete espécies. A temperatura média mensal, a pluviosidade e a temperatura na noite de coleta influenciaram significativamente as quantidades de Lu. ayrozai e os dois primeiros fatores influenciaram Lu. geniculata, além das quantidades de fêmeas coletadas de ambas as espécies. A influência do aumento dos fatores nas quantidades de Lu. ayrozai foi mais imediata que nas de Lu. geniculata. As quantidades coletadas de ambas as espécies e das fêmeas de Lu. geniculata nas estações, mas não nas alturas, foram significativamente diferentes. As diferenças entre os comportamentos de Lu. ayrozai em Morretes e em outras regiões poderiam ser atribuídas a diferenças ambientais e/ou a variações regionais na espécie, que poderia constituir um complexo de espécies. As variações horárias nas coletas foram diferentes nas espécies e estações.

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“…Collection chambers of traps were replaced at two hour intervals, the change being completed within 2-3 min. Sandflies were prepared for identification by NC method 34 .…”

Section: Methodsmentioning

confidence: 99%

Ecology of Phlebotomine sandflies (Diptera, Psychodidae) in Brazilian Atlantic Forest

Marcondes

Santos-Neto

Lozovei

2001

Rev. Soc. Bras. Med. Trop.

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“…Se muestrearon 10 viviendas del barrio Lo Amador; en cada vivienda se hicieron muestreos en el intra y peridomicilio. Los flebótomos recolectados se preservaron en alcohol al 70 %, se aclararon con hidróxido de potasio al 10 % y fenol líquido; a las hembras se les aplicó la técnica de disección de genitales descrita por Marcondes [13] y se utilizó la clave de Young y Duncan para su tipificación [14].…”

Section: Estudio Entomológicounclassified

Cartagena: nuevo foco de leishmaniasis visceral urbana en Colombia.

et al. 2016

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En Colombia, la leishmaniasis visceral (LV) es de predominio rural, en áreas determinadas de algunosdepartamentos de la Región Caribe y en el valle medio del río Magdalena. Una niña de un año de edad, del área urbana de Cartagena, presentó síntomas de hepato-esplenomegalia, anemia, leucopenia y desnutrición, ella no había estado en áreas de riesgo para LV, tuvo pruebas serológicas positivas, reversó síntomas con Glucantime, y luego de tres meses postratamiento su leishmanina fue positiva. Identificar la presencia de vectores, reservorios y condiciones ambientales para la transmisión de LV en el área del caso confirmado. Se realizaron estudios epidemiológicos, clínicos, inmunológicos y moleculares para buscar otros pacientes, reservorios y vectores. El área de estudio presentó condiciones que favorecen la transmisión de la enfermedad. No se encontraron otros niños enfermos. Siete personas adultas tuvieron resultado reactivo a la Prueba Intradérmica de Montenegro, indicando contacto previo con el parásito, y dentro de su vivienda se capturó Lu. evansi. En un canino y en un Didelphis marsupialis se confirmó por laboratorio la infección por Leishmania infantum. Se demostró un foco urbano y nuevo de LV en Colombia que debe ser objeto de acciones regulares de salud pública.

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New Records of Phlebotomine Sand Flies (Diptera: Psychodidae) From the Western Brazilian Amazon and the Description of the Female of Pintomyia fiocruzi

Costa

Júnior

Pessoa

et al. 2020

Journal of Medical Entomology

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Abstract An entomological survey was conducted in several localities of Rondônia State, and new records were obtained for seven sand fly species: Evandromyia apurinan Shimabukuro, Figueira & Silva, 2013, Evandromyia carmelinoi (Ryan, Fraiha, Lainson & Shaw, 1986), Micropygomyia echinatopharynx Andrade-Filho, Galati, Andrade & Falcão, 2004, Nyssomyia urbinattii Galati & Galvis, 2012, Pintomyia duckei Oliveira, Alencar & Freitas, 2018, Psathyromyia pradobarrientosi (Le Pont, Matias, Martinez & Dujardin, 2004), and Sciopemyia vattierae (Le Pont & Desjeux, 1992). Herein, we also describe the female of Pintomyia fiocruzi Pereira Júnior, Pessoa, Marivalva & Medeiros, 2019. The female has spermathecae with a detached apical ring similar to the spermathecae of Pintomyia serrana (Damasceno & Arouck, 1949), Pintomyia odax (Fairchild & Hertig, 1961), Pintomyia ottolinai (Ortiz & Scorza, 1963), and Pintomyia robusta (Galati, Cáceres & Le Pont, 1995). Here, we provide characters to separate Pi. fiocruzi from Pi. serrana and Pi. odax. Pintomyia ottolinai has a trans-Andean distribution and Pi. robusta has not been found in Brazil and in our studies, we associated the females found in this study with Pi. fiocruzi males found at the same collection sites. Our findings highlight the importance of entomological fauna surveys and demonstrate the need for taxonomists to study species diversity in Rondônia. This study increases the number of sand fly species recorded in Rondônia to 143.

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An Improved Technique for the Dissection of Female Genitalia of Phlebotomine Sandflies (Diptera: Psychodidae), with an Improvement in the Handling of Insects

Cited by 5 publications

References 0 publications

Ecology of Phlebotomine sandflies (Diptera, Psychodidae) in Brazilian Atlantic Forest

Ecology of Phlebotomine sandflies (Diptera, Psychodidae) in Brazilian Atlantic Forest

Cartagena: nuevo foco de leishmaniasis visceral urbana en Colombia.

New Records of Phlebotomine Sand Flies (Diptera: Psychodidae) From the Western Brazilian Amazon and the Description of the Female of Pintomyia fiocruzi

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