Genetic Variation among Natural and Laboratory Colony Populations of Lutzomyia longipalpis (Lutz &amp; Neiva, 1912)(Diptera:Psychodidae) from Colombia

Lanzaro, Gregory C.; Alexander, Bruce; Mutebi, John Paul; Montoya-Lerma, James; Warburg, Alon

doi:10.1590/s0074-02761998000100013

Cited by 25 publications

(20 citation statements)

References 11 publications

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“…Recently, isozyme surveys of field populations have been conducted in Colombia, Central America, and Brazil. Munstermann and others 27 and Lanzaro and others 28 found very low genetic variability and little divergence among field populations in Colombia, consistent with local populations within the same species. Similarly, Mutebi and others 29 found very low genetic variability and little divergence among 11 populations from three countries in Central America: Honduras, Costa Rica, and Nicaragua.…”

supporting

confidence: 68%

Breeding structure of the sand fly Lutzomyia longipalpis (Lutz & Neiva) in Brazil.

Mutebi¹,

Alexander²,

Sherlock³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. Eleven populations of Lutzomyia longipalpis (Lutz & Neiva), the sand fly vector of Leishmania chagasi, from different areas of Brazil were analyzed for genetic variation at 16 enzyme loci. In this region, the prevalence of visceral leishmaniasis (VL) caused by L. chagasi is spotty and reproductive isolation among populations of Lu. longipalpis has been reported. It is thought that morphologically similar cryptic species with varying vectorial capacity may be responsible for the discontinuous distribution of VL. The aim was to study the genetic structure of populations within this region and to identify demes that may represent sibling species. Genotypic frequencies within populations were in close compliance to Hardy-Weinberg expectations, suggesting there are no sympatric species among these 11 populations. Levels of genetic distance between pairs of populations were very low (Ͻ 0.03), consistent with local populations within a single sand fly species. When genotypic frequency data for all populations were pooled, 9 of the 13 polymorphic loci deviated from Hardy-Weinberg expectations, indicating some degree of genetic substructuring. Estimates of effective migration rates (N e m) among all populations were low, 2.73, suggesting that gene flow is restricted among populations, which is probably the reason for the observed genetic substructuring.

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supporting

confidence: 68%

Breeding structure of the sand fly Lutzomyia longipalpis (Lutz & Neiva) in Brazil.

Mutebi¹,

Alexander²,

Sherlock³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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“…longipalpis (originating from a colony established with material coming from Belo Horizonte, Brazil) gave different results. This suggests that, in cell cultures, the species (derived from two South American populations) could also present genetic polymorphism, sustaining the idea that it is a complex of species (Ward et al 1983, Lanzaro et al 1993, 1998.…”

Section: Discussionmentioning

confidence: 68%

Establishment and characterization of a new continuous cell line from Lutzomyia longipalpis (Diptera: Psychodidae) and its susceptibility to infections with arboviruses and Leishmania chagasi

Rey

Ferro

Bello

2000

Mem. Inst. Oswaldo Cruz

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“…Recently, such studies have begun, focusing on Phlebotomus papatasi (Scopoli) in the Old World 32 and several Lutzomyia species in the New World, including the L. townsendi Series, 33 L. carrerai/ yucumensis, 34 and L. longipalpis. 19,22,[35][36][37][38] The present report describes enzyme polymorphisms and their frequencies in widely distributed field populations of L. longipalpis across its most concentrated distribution, i.e., in Brazil.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability in biochemical characters of Brazilian field populations of the Leishmania vector, Lutzomyia longipalpis (Diptera: Psychodidae).

Mukhopadhyay¹,

Ghosh²,

Rangel³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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Abstract. The phlebotomine sand fly Lutzomyia longipalpis is the insect vector of visceral leishmaniasis, a protozoan disease of increasing incidence and distribution in Central and South America. Electrophoretic allele frequencies of 15 enzyme loci were compared among the L. longipalpis populations selected across its distribution range in Brazil. The mean heterozygosity of two colonized geographic strains (one each from Colombia and Brazil) were 6% and 13% respectively, with 1.6-1.9 alleles detected per locus. In contrast, among the seven widely separated field populations, the mean heterozygosity ranged from 11% to 16% with 2.1-2.9 alleles per locus. No locus was recovered that was diagnostic for any of the field populations. Allelic frequency differences among five field strains from the Amazon basin and eastern coastal Brazil were very low, with Nei's genetic distances of less than 0.01 separating them. The two inland and southerly samples from Minas Gerais (Lapinha) and Bahia (Jacobina) states were more distinctive with genetic distances of 0.024-0.038 and 0.038-0.059, respectively, when compared with the five other samples. These differences were the consequence of several high frequency alleles (glycerol-3-phosphate dehydrogenase [Gpd 1.69 ] and phosphoglucomutase [Pgm 1.69 ]) relatively uncommon in other strains. The low genetic distances, absence of diagnostic loci, and the distribution of genes in geographic space indicate L. longipalpis of Brazil to be a single, but genetically heterogeneous, polymorphic species.American visceral leishmaniasis (kala-azar) is a fatal disease in the New World tropics. Approximately 1.6 million people are at risk of infection with this disease and on average, 16,000 cases are reported annually. 1,2 More than 90% of human cases occur in Brazil. 3,4 Visceral leishmaniasis in humans is caused by the kinetoplastid protozoan Leishmania donovani chagasi Cunha and Chagas. 5,6 However, in Central America, infections also frequently result in cutaneous disease. [7][8][9] The most important vector of Leishmania d. chagasi is the phlebotomine sand fly Lutzomyia longipalpis (Lutz and Neiva). 10-13 It was first described from specimens collected in São Paulo Bosque de Saúde (no longer in existence), 100 km north northwest of Belo Horizonte at the Caves of Maquiné (Cordisburgo) and at a ranch near the river port of Benjamin Constant (Além Paraíba, 140 km northeast of Rio de Janeiro) Fazenda Ouro Fino. 14,15 It occurs from southern Mexico to northern Argentina in discontinuous distributions. 16 In Brazil, L. longipalpis has been recorded in habitats ranging from heavily populated urban centers to rural, periurban areas in a wide variety of ecotopes including mountain ranges to the east and the Amazon river basin covering most of the north and central Brazil. Therefore, the sand flies survive in a variety of habitats and sometimes in zones where formidable barriers prevent their migration. Since they have a limited flight range, rarely migrating more than 100 meters, the widely separ...

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Genetic Variation among Natural and Laboratory Colony Populations of Lutzomyia longipalpis (Lutz & Neiva, 1912)(Diptera:Psychodidae) from Colombia

Abstract: Genetic diversity among three field populations of

Cited by 25 publications

References 11 publications

Breeding structure of the sand fly Lutzomyia longipalpis (Lutz & Neiva) in Brazil.

Breeding structure of the sand fly Lutzomyia longipalpis (Lutz & Neiva) in Brazil.

Establishment and characterization of a new continuous cell line from Lutzomyia longipalpis (Diptera: Psychodidae) and its susceptibility to infections with arboviruses and Leishmania chagasi

Genetic variability in biochemical characters of Brazilian field populations of the Leishmania vector, Lutzomyia longipalpis (Diptera: Psychodidae).

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