Specific Identification of Biomphalaria tenagophila and Biomphalaria occidentalis Populations by the Low Stringency Polymerase Chain Reaction

Further studies on the molecular systematics of Biomphalaria snails from Brazil

“…To overcome this problem, the use of molecular techniques as additional tools for the identification of these snails has been proposed. In previous studies we tested a low stringency polymerase chain reaction technique (LS-PCR) (Dias Neto et al 1993) which permited the differentiation of B. glabrata and B. t. tenagophila and also the identification of very similar species such as B. t. tenagophila and B. occidentalis (Vidigal et al 1996, Pires et al 1997.…”

Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)

“…Species abbreviations are as defined in Table. DISCUSSION In previous studies, using different molecular techniques, we reported a high degree of intra-specific variability in several species of Biomphalaria from different Brazilian localities, as well as from Uruguay and Argentina (Vidigal e al. 1994, 1996, 1998, Pires et al 1997, Caldeira et al 2001, Campos et al 2002. This variability is also observed when morphological characters such as vaginal pouch, vaginal wrinkling, penis sheath, prepuce, and prostatic diverticula are carefully analyzed (Paraense 1975(Paraense , 1981(Paraense , 1984(Paraense , 1888.…”

Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni

“…These techniques include procedures such as analysis of the region 18S of rRNA through low stringenecy polymerase chain reaction (LS-PCR) (Vidigal et al 1996, Pires et al 1997, polymerase chain reaction and restriction fragment length analysis (PCR-RFLP) of the internal transcribed spacer region of the DNA (ITSr-DNA) (Vidigal et al 1998, Caldeira, 1998, Spatz et al 1999. However, high intraespecific polymorphisms and complex profiles, produced by such methodologies, may restrict their use.…”