Influence of the route of administration of pig-serum in the induction of hepatic septal fibrosis in rats

Andrade, Zilton A.; Godoy, Adriana

doi:10.1590/s0074-02761996000600023

Cited by 3 publications

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“…Also, for the process of immunological tolerance it is important that the antigens remain for a prolonged period of time within the organism, otherwise only a transient tolerance would be induced (21). To emphasize the importance of the route of administration, Andrade and Godoy (22) demonstrated that rats treated with whole pig serum by subcutaneous injections failed to develop hepatic fibrosis. Under the same experimental protocol, septal hepatic fibrosis developed in 65% of the rats injected ip.…”

Section: Discussionmentioning

confidence: 99%

Immunological basis of septal fibrosis of the liver in Capillaria hepatica-infected rats

Lemos

Magalhães-Santos

Andrade

2003

Braz J Med Biol Res

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Rats infected with the helminth Capillaria hepatica regularly develop septal fibrosis of the liver similar to that induced by repeated ip injections of pig serum. Fibrosis starts when the focal parasitic lesions begin to show signs of resorption, thus suggesting an immunologically mediated pathogenesis of this fibrosis. To explore this possibility, the development of C. hepatica-related hepatic fibrosis was observed in rats exposed to worm antigens from the first neonatal day onward. Wistar rats (150 g) were either injected ip with an extract of C. hepatica eggs (protein concentration: 1 mg/ml) or received immature eggs by gavage from the first neonatal day until adult life and were then infected with 500 embryonated eggs. Changes were monitored on the basis of serum levels of anti-worm antibodies and hepatic histopathology. Rats submitted to immunological oral tolerance markedly suppressed C. hepatica-related serum antibodies and septal fibrosis of the liver when infected with the helminth later on. Tolerance trials with ip injections of worm antigens gave essentially negative results. The partial suppression of septal fibrosis of the liver after the induction of immunological tolerance to C. hepatica antigens in rats indicates an immunological basis for the fibrosis and emphasizes the importance of immunological factors in the pathogenesis of hepatic fibrosis.

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Section: Discussionmentioning

confidence: 99%

Immunological basis of septal fibrosis of the liver in Capillaria hepatica-infected rats

Lemos

Magalhães-Santos

Andrade

2003

Braz J Med Biol Res

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Improved antifibrotic effect of a combination of verapamil and silymarin in rat-induced liver fibrosis

Shafik

Khodeir

Gouda

et al. 2011

Arab Journal of Gastroenterology

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Production of septal fibrosis of the liver by means of foreign protein injections into rats

Gotardo¹,

Andrade²,

Oliveira³

et al. 2003

Rev. Soc. Bras. Med. Trop.

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Similarities and differences in antigenic humoral responses and electrophoretic patterns between Capillaria hepatica and pig-serum were investigated as a contribution to the understanding of hepatic fibrosis induced by the parenteral administration of foreign proteins. Only two out of 10 rats receiving repeated intraperitoneal injections of an extract of Capillaria hepatica-infected mouse liver presented septal hepatic fibrosis (20%). Under the same experimental conditions, 4 out of 9 rats (44.4%) developed septal fibrosis following whole pig-serum administration. Injections of normal mouse liver extracts did not result in hepatic fibrosis. Since a 100% septal fibrosis rate is observed in experimentally Capillaria hepatica-infected rats, it appeared that Capillaria hepatica products continuously released from inside the liver creates a much more effective fibrosis inducing mechanism than the parenteral administration of such factors. Thus, repeated peritoneal administration of a foreign protein to rats would not reveal the full fibrogenic potential it may have under natural conditions. Key-words: Capillaria hepatica. Hepatic fibrosis. Pig-serum model.Resumo Para se investigar o potencial fibrogênico de materiais derivados da Capillaria hepatica foi feita uma tentativa para se induzir fibrose hepática septal em ratos, através de injeções intraperitoneais repetidas de um extrato de fígado de camundongo infectado pela Capillaria hepática. Dois dos 10 ratos assim tratados desenvolveram fibrose. Comparativamente, 4 dos 9 ratos que receberam injeções de soro de porco desenvolveram fibrose septal do fígado, enquanto nenhuma fibrose apareceu em 10 ratos tratados com extratos de fígado normal de camundongo. Uma vez que, na infecção natural de ratos pela Capillaria hepatica, a fibrose aparece em 100% dos casos, fica evidente que a administração intraperitoneal de uma substância não revela inteiramente o seu potencial fibrogênico. Palavras-chaves: Capillaria hepatica. Fibrose septal hepática. Modelo do soro de porco.

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Influence of the route of administration of pig-serum in the induction of hepatic septal fibrosis in rats

Cited by 3 publications

References 0 publications

Immunological basis of septal fibrosis of the liver in Capillaria hepatica-infected rats

Immunological basis of septal fibrosis of the liver in Capillaria hepatica-infected rats

Improved antifibrotic effect of a combination of verapamil and silymarin in rat-induced liver fibrosis

Production of septal fibrosis of the liver by means of foreign protein injections into rats

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