Comparison of purified 12 kDa and recombinant 15 kDa Fasciola hepatica antigens related to a Schistosoma mansoni fatty acid binding protein

Abstract

Vaccines in schistosomiasis using homologous antigens have been studied extensively in experimentally infected mammalian hosts. Vaccines using heterologous antigens have received comparatively less attention. This review summarizes recent work on a heterologous 12 kDa Fasciola hepatica antigenic polypeptide which cross reacts with Schistosoma mansoni. A cDNA has been cloned and sequenced, and the predicted amino acid sequence of the recombinant protein has been shown to have significant (44%) identity with a 14 kDa S. mansoni fatty acid binding protein. Thus in the parasitic trematodes fatty acid binding proteins may be potential vaccine candidates. The F. hepatica recombinant protein has been overexpressed and purified and denoted rFh15. Preliminary rFh15 migrates more slowly (i.e. may be slightly larger) than nFh12 on SDS-PAGE and has a predicted pI of 6.01 vs. observed pI of 5.45. Mice infected with F. hepatica develop antibodies to nFh12 by 2 weeks of infection vs. 6 weeks of infection to rFh15; on the other hand, mice with schistosomiasis mansoni develop antibodies to both nFh12 and rFh15 by 6 weeks of infection. Both the F. hepatica and S. mansoni cross-reactive antigens may be cross-protective antigens with the protection inducing capability against both species.

Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice

“…Vaccines in schistosomiasis using homologous antigens have been studied extensively in experimentally infected mammalian hosts; vaccines using heterologous antigens have received comparatively less attention [20]. Cross immunity has been demonstrated among Schistosoma species and different parasitic trematodes [21-25].…”

“…Cross immunity has been demonstrated among Schistosoma species and different parasitic trematodes [21-25]. One of the most studied cross reactive antigens is the native Fh12 and recombinant rFh15 F. hepatica FABP [20]. Sirisriro et al [9] developed mAb against rFABP of F. gigantica .…”

Mapping of B-Cell Epitopes on a Novel 11.5-KilodaltonFasciola hepatica-Schistosoma mansoniCross-Reactive Antigen Belonging to a Member of theF. hepaticaSaposin-Like Protein Family

“…Cross-reactivity between F. hepatica and S. mansoni antigens has been repeatedly demonstrated in serological assays (12,16), suggesting the existence of ancestral genes conserved in the phylogeny of both species. The identification of these cross-reactive epitopes in the FhSAP-2 protein strongly suggests that the regions 21 SKQPTIDIDL 30 and 76 QQEVDYI IDH 85 of FhSAP-2 could be regions conserved between both species and could suggest the existence of a homolog of protein FhSAP-2 in S. mansoni.…”

Native and Recombinant Fatty Acid Binding Protein 3 fromFasciola Hepaticaas a Potential Antigen

“…Many researchers have studied FABPs from F. hepatica as vaccine candidate (4,9,12). That is the reason why this paper reports the investigation of native and recombinant F. hepatica FABP3 and its possible role in chemotherapy and as a diagnostic antigen.…”