Integrated control measures against Culex quinquefasciatus, the vector of filariasis in Recife

Regis, Lêda; Silva-Filha, Maria Helena Neves Lobo; Oliveira, Conceição Maria de; Rios, Eugênia Maria; Silva, S B da; Furtado, André Freire

doi:10.1590/s0074-02761995000100022

Cited by 62 publications

(42 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In equatorial-tropical regions, the species is reported as the primary vector of Wuchereria bancrofti in the transmission cycle of lymphatic filariasis 36 . In subtropical and temperate urban areas, it is implicated as the primary vector of West Nile Virus 23 and other arboviruses 11 .…”

Section: Introductionmentioning

confidence: 99%

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Morais

Almeida

Suesdek

et al. 2012

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYCulex quinquefasciatus is a vector of human pathogens, including filarial nematodes and several viruses. Although its epidemiological relevance is known to vary across geographical regions, an understanding of its population genetic structure is still incipient. In light of this, we evaluated the genetic diversity of Cx. quinquefasciatus and Cx. pipiens x Cx. quinquefasciatus hybrids collected from nine localities in Brazil and one site in Argentina. We used mitochondrial genes cox1 and nd4, along with the coxA and wsp genes of the maternally-inherited Wolbachia endosymbiont. The nd4 fragment was invariant between samples, whilst cox1 exhibited four haplotypes that separated two types of Cx. quinquefasciatus, one clustered in southern Brazil. Low sequence diversity was generally observed, being discussed. Both Brazilian and Argentinian mosquitoes were infected with a single Wolbachia strain. As reported in previous studies with these populations, cox1 and nd4 diversity is not congruent with the population structure revealed by nuclear markers or alar morphology. Future Cx. quinquefasciatus research should, if possible, evaluate mtDNA diversity in light of other markers.

show abstract

Section: Introductionmentioning

confidence: 99%

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Morais

Almeida

Suesdek

et al. 2012

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…Yet Orlova et al (21) demonstrated that crystalliferous strains of B. laterosporus presented LC 50 values similar to those attained with Bacillus thuringiensis serovar israelensis in bioassays employing larvae of three species of mosquitoes, with the larvicidal activity of B. laterosporus being associated with spores and crystalline inclusions. Although Bacillus thuringiensis and Bacillus sphaericus have been used as biological control agents in many countries (4,18,23,24), some strains still present problems, including low environmental persistence and a restricted range of targets. Thus, the availability of alternative agents would be highly desirable.…”

mentioning

confidence: 99%

Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

Oliveira¹,

Rabinovitch²,

Monnerat

et al. 2004

Appl Environ Microbiol

127

View full text Add to dashboard Cite

Thirty-three strains of Brevibacillus laterosporus, including three novel strains isolated from Brazilian soil samples, were examined for genetic variability by the use of different PCR-based methods. Molecular markers that could characterize bacterial strains with regards to their pathogenic potential were investigated. In addition, toxicity was assessed by the use of insects belonging to the orders Lepidoptera and Coleoptera and the mollusk Biomphalaria glabrata. Among the targets tested, Biomphalaria glabrata demonstrated the highest degree of sensitivity to B. laterosporus, with some strains inducing 90 to 100% mortality in snails aged 3 and 12 days posteclosion. Larvae of the coleopteron Anthonomus grandis were also susceptible, presenting mortality levels of between 33 and 63%. Toxicity was also noted towards the lepidopteron Anticarsia gemmatalis. In contrast, no mortality was recorded among test populations of Tenebrio molitor or Spodoptera frugiperda. The application of intergenic transcribed spacer PCR and BOX-PCR generated 15 and 17 different genotypes, respectively. None of the molecular techniques allowed the identification of a convenient marker that was associated with any entomopathogenic phenotype. However, a 1,078-bp amplicon was detected for all strains of B. laterosporus when a primer for amplification of the BOXA1R region was used. Similarly, a 900-bp amplicon was generated from all isolates by use of the primer OPA-11 for randomly amplified polymorphic DNA analysis. These amplicons were not detected for other phenotypically related Brevibacillus species, indicating that they represent markers that are specific for B. laterosporus, which may prove useful for the isolation and identification of new strains of this species.

show abstract

“…and Anopheles sp. larvae, showing specific efficacy and feasibility as an effective tool for controlling vector populations (Karch et al, 1992;Kumar et al, 1994Kumar et al, , 1996Regis et al, 1994). B. sphuericus displays high toxicity against Cu1e.x pipiens and variable toxicity levels against Anopheles and Aedes, according to the species and to the bacterial strain.…”

mentioning

confidence: 99%

Binding Kinetics of Bacillus Sphaericus Binary Toxin to Midgut Brush‐Border Membranes of Anopheles and Culex sp. Mosquito Larvae

Silva-Filha

Nielsen-LeRoux

Charles

1997

European Journal of Biochemistry

View full text Add to dashboard Cite

Direct-binding assays and homologous-competition assays were used to identify specific binding between the radiolabelled toxin of Bacillus sphaericus and brush-border membrane fractions (BBMF) from Anopheles gambiae and Anopheles stephensi, obtained from whole larvae preparations. In both species, the toxin bound to a single class of receptors. BBMF of A. gunzbiae had the highest binding affinity for the toxin of the species tested, with a dissociation constant ( K J of 30 2 15 nM and a maximum receptor concentration of 5 2 1 pmol/mg. Toxin binding to A. gambiae BBMF was compared with that to BBMF from B. sphaericus-susceptible (IP) and B. sphaericus-resistant (SPHAE) Culex pipiens populations. BBMF toxin binding was slower in A. gambiae than in the C. pipiens populations. The BBMF of the B. sphaericus-resistant population of C. pipiens had an association profile that was similar to the susceptible population, despite of the lack of susceptibility in vivo. No relationship between toxicity and irreversibility of toxin binding was detected. On the contrary, toxin dissociation from BBMF was fast and almost complete in BBMF of all species studied.

show abstract

Integrated control measures against Culex quinquefasciatus, the vector of filariasis in Recife

Cited by 62 publications

References 0 publications

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

Binding Kinetics of Bacillus Sphaericus Binary Toxin to Midgut Brush‐Border Membranes of Anopheles and Culex sp. Mosquito Larvae

Contact Info

Product

Resources

About