CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection

Abstract

We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania.

MHC Class II Restricted Innate-Like Double Negative T Cells Contribute to Optimal Primary and Secondary Immunity to Leishmania major

“…Previous studies that have reported the expansion and possible protective role of DN T cells in leishmaniasis focused mainly on primary Leishmania infection [11], [12], [13], [14]. We extend these studies during secondary immunity by showing rapid expansion and effector functions (cytokine production and parasite control) by DN T cells following challenge infection.…”

“…However, the finding that CD4 deficient mice were resistant while MHC class II deficient mice were highly susceptible to L. major challenged this dogma [10] and suggests that MHC II-restricted CD4 − CD8 − T cells may be more important in regulating primary anti- Leishmania immunity. Indeed, several studies have reported the expansion of CD3 + CD4 − CD8 − (DN) T cells in the blood of Leishmania -infected patients and dogs, and in spleens of Leishmania -infected mice [11], [12], [13], [14]. These cells have been proposed to contribute to primary and vaccine-induced immunity although a concrete evidence implicating them in immunity has not yet been demonstrated.…”

“…In addition, several studies have reported increased numbers of DN T cells in blood of Leishmania -infected patients [11], [12], dogs [13], and in spleens of Leishmania -infected mice [14]. These cells have been proposed to contribute to primary and vaccine-induced immunity against Leishmania .…”

Disparate Immunoregulatory Potentials for Double-Negative (CD4−CD8−) αβ and γδ T Cells from Human Patients with Cutaneous Leishmaniasis

“…ϩ , CD8 ϩ , or CD4 Ϫ CD8 Ϫ T cells from vaccinated or infected donors conferred significant disease resistance when they were transferred to naïve recipients and then infected with Leishmania mexicana (19).…”

Systemic cytokine response in humans with chiclero’s ulcers

“…An indirect noncompetitive technique on solid phase was chosen (Ngo and Lenhof 1981). Detailed explanation of this procedure is found in previous reports (Lezama-Davila and Gallagher 1995;Lezama-Davila et al 1998). Data are expressed as OD (optical density) of experimental sample/OD of negative control (Lezama-Davila and Gallagher 1995).…”