Correlation between anti-V3 peptide and neutralizing antibodies in plasma from HIV-1 infected individuals resident in Brazil

Bongertz,; Masucci, Maria G.; Galvão–Castro, Bernardo; Wigzell, Hans; Rm, Hendry

doi:10.1590/s0074-02761994000100019

Cited by 3 publications

(1 citation statement)

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“…These results agree with data from other Brazilian studies (3,14), as well as with studies from other countries (33-35); however, they disagree with other studies in which a correlation was observed between neutralization and seroreactivity against the peptide ELDKWA (36), peptides from gp120 and gp41 (37), and peptides from the V3 loop (38,39).…”

Section: Discussionsupporting

confidence: 83%

HIV-1 binding and neutralizing antibodies of injecting drug users

Ouverney

Teixeira

Jesus

et al. 2005

Braz J Med Biol Res

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Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed. Seroreactivity indicates that IDU plasma are more likely to recognize a broader range of peptides than S plasma, with significantly higher titers, especially of V3 peptides. Similar neutralization frequencies of the MN isolate were observed in plasma of the IDU (16/47) and S (20/60) groups in the 1:10 dilution. The neutralization of the 95BRRJ021 isolate was more frequently observed for plasma from the S group (15/23) than from the IDU group (15/47, P = 0.0108). No correlation between neutralization and seroreactivity with the peptides tested was observed. These results suggest that an important factor responsible for the extensive and broad humoral immune response observed in IDU is their infection route. There was very little difference in neutralizing antibody response between the IDU and S groups despite their differences in seroreactivity and health status.

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