Mayaro virus proteins

Mezêncio, J. M. S.; Rebello, M. A.

doi:10.1590/s0074-02761993000200020

Cited by 14 publications

(5 citation statements)

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“…The genome of MAYV is a single-stranded, positive sense RNA, approximately 11.45 Kb in length that encodes 4 nonstructural proteins (nsP1-4) on the 5′ end and 3 structural proteins on the 3′ end, including the capsid and envelope glycoproteins, E1 and E2 (Fig. 1) [13], [15]. Genomic RNA includes 2 open reading frames (ORFs); the nonstructural polyprotein ORF is translated in a cap-dependent manner from genomic RNA, while the structural polyprotein ORF is translated from a subgenomic RNA transcript, which is also capped [16], [17].…”

Section: Introductionmentioning

confidence: 99%

A Novel Live-Attenuated Vaccine Candidate for Mayaro Fever

et al. 2014

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Mayaro virus (MAYV) is an emerging, mosquito-borne alphavirus that causes a dengue-like illness in many regions of South America, and which has the potential to urbanize. Because no specific treatment or vaccine is available for MAYV infection, we capitalized on an IRES-based approach to develop a live-attenuated MAYV vaccine candidate. Testing in infant, immunocompetent as well as interferon receptor-deficient mice demonstrated a high degree of attenuation, strong induction of neutralizing antibodies, and efficacy against lethal challenge. This vaccine strain was also unable to infect mosquito cells, a major safety feature for a live vaccine derived from a mosquito-borne virus. Further preclinical development of this vaccine candidate is warranted to protect against this important emerging disease.

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Section: Introductionmentioning

confidence: 99%

A Novel Live-Attenuated Vaccine Candidate for Mayaro Fever

et al. 2014

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“…MAYV was propagated and purified as previously described (Mezencio & Rebello, 1993) with several modifications. BHK-21 cells were grown to quasi-confluence in 300-cm 2 flasks (TPP, Trasadingen, Switzerland) and then infected with MAYV under a multiplicity of infection (MOI) of 0.1 plaque-forming unit/cell (PFU/cell) for 48 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

On the entry of an emerging arbovirus into host cells: Mayaro virus takes the highway to the cytoplasm through fusion with early endosomes and caveolae-derived vesicles

Carvalho

Silva

Oliveira

et al. 2017

PeerJ

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Mayaro virus (MAYV) is an emergent sylvatic alphavirus in South America, related to sporadic outbreaks of a chikungunya-like human febrile illness accompanied by severe arthralgia. Despite its high potential for urban emergence, MAYV is still an obscure virus with scarce information about its infection cycle, including the corresponding early events. Even for prototypical alphaviruses, the cell entry mechanism still has some rough edges to trim: although clathrin-mediated endocytosis is quoted as the putative route, alternative paths as distinct as direct virus genome injection through the cell plasma membrane seems to be possible. Our aim was to clarify crucial details on the entry route exploited by MAYV to gain access into the host cell. Tracking the virus since its first contact with the surface of Vero cells by fluorescence microscopy, we show that its entry occurs by a fast endocytic process and relies on fusion with acidic endosomal compartments. Moreover, blocking clathrin-mediated endocytosis or depleting cholesterol from the cell membrane leads to a strong inhibition of viral infection, as assessed by plaque assays. Following this clue, we found that early endosomes and caveolae-derived vesicles are both implicated as target membranes for MAYV fusion. Our findings unravel the very first events that culminate in a productive infection by MAYV and shed light on potential targets for a rational antiviral therapy, besides providing a better comprehension of the entry routes exploited by alphaviruses to get into the cell.

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“…MAYV was propagated in BHK-21 cells for 48 h at 37 °C. The virus was purified as described previously, 16 with some modifications. After propagation, the supernatant was collected and cleared of cellular debris by centrifugation at 8000 rpm for 20 min at 4 °C in a Hitachi RPR 12-2 rotor.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Fusion of a New World Alphavirus with Membrane Microdomains Involving Partially Reversible Conformational Changes in the Viral Spike Proteins

et al. 2017

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Alphaviruses are enveloped arboviruses mainly proposed to infect host cells by receptor-mediated endocytosis followed by fusion between the viral envelope and the endosomal membrane. The fusion reaction is triggered by low pH and requires the presence of both cholesterol and sphingolipids in the target membrane, suggesting the involvement of lipid rafts in the cell entry mechanism. In this study, we show for the first time the interaction of an enveloped virus with membrane microdomains isolated from living cells. Using Mayaro virus (MAYV), a New World alphavirus, we verified that virus fusion to these domains occurred to a significant extent upon acidification, although its kinetics was quite slow when compared to that of fusion with artificial liposomes demonstrated in a previous work. Surprisingly, when virus was previously exposed to acidic pH, a condition previously shown to inhibit alphavirus binding and fusion to target membranes as well as infectivity, and then reneutralized, its ability to fuse with membrane microdomains at low pH was retained. Interestingly, this observation correlated with a partial reversion of low pH-induced conformational changes in viral proteins and retention of virus infectivity upon reneutralization. Our results suggest that MAYV entry into host cells could alternatively involve internalization via lipid rafts and that the conformational changes triggered by low pH in the viral spike proteins during the entry process are partially reversible.

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Mayaro virus proteins

Cited by 14 publications

References 0 publications

A Novel Live-Attenuated Vaccine Candidate for Mayaro Fever

A Novel Live-Attenuated Vaccine Candidate for Mayaro Fever

On the entry of an emerging arbovirus into host cells: Mayaro virus takes the highway to the cytoplasm through fusion with early endosomes and caveolae-derived vesicles

Fusion of a New World Alphavirus with Membrane Microdomains Involving Partially Reversible Conformational Changes in the Viral Spike Proteins

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