Recent advances and prospective researches on molecular epidemiology of dengue viruses

Denis, Vincent

doi:10.1590/s0074-02761992000900021

Cited by 3 publications

(3 citation statements)

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“…However, it took some years for the new subtype to replace the original one, as evidenced by the presence of the "native" subtype (F) in Mexico in 1983 (lane 7). As another example, the previously proposed hypothesis that dengue fever in Burkina Faso (1982) was caused by strains that had originated in Sri Lanka or India (Deubel, 1992;Rico-Hesse, 1990) is consistent with the RSS-PCR classification of both Sri Lankan strains (1982)(1983)(1984)(1985) and a 1982 isolate from Burkina Faso as RSS-PCR type E2 (Table 4).…”

Section: Epidemiologic and Clinical Correlations Of Dengue-2 Rss-pcr supporting

confidence: 77%

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Rapid Subtyping of Dengue Viruses by Restriction Site-Specific (RSS)–PCR

Harris

Sandoval²,

Xet-Mull

et al. 1999

Virology

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Dengue is a major public health problem worldwide. It is caused by four dengue virus serotypes, each further divided into distinct genetic subtypes. Strain typing is important for understanding the epidemiology and viral factors associated with disease transmission. However, most of the existing subtyping methods are expensive and technically unwieldy for timely, practical applications in developing countries. Here we describe a simple, rapid, PCR-based subtyping method, restriction site-specific (RSS)-PCR, which we used to analyze dengue virus serotypes 2 and 3. For each serotype, four primers targeted to sequences spanning polymorphic endonuclease restriction sites in the envelope gene were used to reverse transcribe and amplify viral RNA. These RT-PCR products generated distinct electrophoretic band patterns for different strains. Analysis of 73 dengue-2 strains and 54 dengue-3 strains representing a broad geographic distribution over several decades revealed that the RSS-PCR fingerprints reproducibly fell into 7 and 3 groups, respectively. These groups correlated well with previous phylogenetic classifications. This one-step assay should be widely accessible and allow more detailed epidemiologic investigations in dengue-endemic countries. This novel PCR approach to subtyping organisms based on restriction site polymorphisms should be applicable to other pathogens.

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Section: Epidemiologic and Clinical Correlations Of Dengue-2 Rss-pcr supporting

confidence: 77%

“…Year type E into West Africa from Sri Lanka also reflects transmission pathways detected by sequencing (Deubel, 1992;Lewis et al, 1993;Rico-Hesse, 1990). The ability to obtain this information rapidly in dengue-endemic countries should enhance our understanding of the global spread of dengue fever.…”

Section: Countrymentioning

confidence: 99%

Rapid Subtyping of Dengue Viruses by Restriction Site-Specific (RSS)–PCR

Harris

Sandoval²,

Xet-Mull

et al. 1999

Virology

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“…For the construction of parsimony trees the E glycoprotein gene would be more suitable. For this reason a specially designed nested RT-PCR procedure (6,25,32) might be applied to our RNA samples. Our results with the detection of viral RNA by using the TaqMan procedure confirm earlier findings showing that dengue virus can be readily detected in early serum samples taken before IgG antibodies are present (2,3).…”

Section: Discussionmentioning

confidence: 99%

Detection of Dengue Virus RNA in Patients after Primary or Secondary Dengue Infection by Using the TaqMan Automated Amplification System

1999

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In consecutive serum samples from 25 tourists with acute dengue fever, virus-specific RNA was detected by using fully automated TaqMan reverse transcriptase PCR. For this amplification technique new primers and special fluorochrome-labeled probes had to be synthesized. During amplification the increasing amount of viral DNA could simultaneously be measured in the tightly sealed tubes. Dengue virus RNA was found in almost all patients (17 of 18), if the samples had been taken soon after the onset of symptoms and before anti-dengue virus antibody had been produced. RNA was detectable in only one of five persons who had anti-dengue virus immunoglobulin M (IgM) antibodies but not yet IgG antibodies. In 30 late samples with both IgG and IgM antibodies viral RNA was no longer demonstrable. In two early samples from two frequent travelers obtained 1 and 2 days after the onset of symptoms significant IgG antibody titers were present but there were no anti-dengue virus IgM antibodies. In these samples a viral load of >5 × 106 dengue virus RNA copies (dengue types 1 and 2) was detectable. These findings of a high viral load in the presence of anti-dengue virus IgG antibody are suggestive of a secondary dengue virus infection. In the 20 tourists (17 plus 1 plus 2) in whom viral RNA was found, the dengue virus serotype could be related to the area where the infection had taken place. Most of our patients came from southeast Asia and most frequently had dengue virus type 1 infections (8 of 20).

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