Amphimerus bragai n.sp. (Digenea, Opisthorchiidae) The genus Amphimerus Barker, 1911 is closely related to Opisthorchis and to Clonorchis from which it differs by having the vitellarium interrupted at the level of the ovary on either side. Species of these genera are thought to be transmitted by the ingestion of fish and most of the known definitive hosts are piscivorous or omnivorous birds and mammals. According to Yamaguti (1971), there are twenty species worldwide, nine from birds, ten from mammals and one from a freshwater turtle. Species of all three of these genera have potential for infecting man.According to Thatcher (1970Thatcher ( , 1993, three species of Amphimerus are known from Brazil and two of these are from mammals, namely: A. guayaquilensis (Rodríguez, Gómez Lince and Montalvan, 1949) Kohn et al. (1981) identified specimens found in the livers of the rodent Nectomys squamipes (Brants, 1827) as A. lancea from the State of Goiás, Brazil. This material has been reexamined and compared to a new collection from the same host. It has been determined that the worms from the two collections are conspecific and represent a new species which is described herein.
MATERIALS AND METHODSThe rodents were live-trapped and brought to the laboratory for examination. They were euthanized with ether and necropsied. The livers were teased apart with dissecting needles and the gall-bladders and common bile ducts were opened. This material was washed in 0.9% physiological saline solution and hand sedimented. The worms were then removed from the saline and fixed. For light microscopy, some specimens were fixed in AFA solution (93 parts of 70% alcohol, 5 parts of commercial formaldehyde and 2 parts of glacial acetic acid). They were then stained with Langeran's carmin, dehydrated in a graded alcohol series and mounted in Canada balsam without compression. All measurements are given in millimiters.Specimens to be examined by scanning electron microscopy (SEM) were fixed in a solution containing 2.5% glutaraldehyde, 4% paraformaldehyde, 5 mM calcium cloride in 0.1M cacodylate buffer (pH 7.2), at room temperature, washed with cacody-