Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings

Goldman, Ira F.; Qari, Shoukat H.; Skinner, Jimmie C.; Oliveira, Salma Gomes de; Nascimento, José Maria; Póvoa, Marinete Marins; Collins, William S.; Lal, Altaf A.

doi:10.1590/s0074-02761992000400019

Cited by 11 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…White blood cells were depleted from the blood by retention on CF11 cellulose (Whatman-no longer available) using a modification of a previously described protocol [ 112 ] ( S1 Text Supplementary methods). RNA was extracted from erythrocytes in TRIzol using a modified RNeasy mini (QIAGEN, Hilden, Germany) protocol ( S1 Text Supplementary methods).…”

Section: Methodsmentioning

confidence: 99%

The Plasmodium falciparum transcriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen–encoding var genes

et al. 2018

View full text Add to dashboard Cite

Within the human host, the malaria parasite Plasmodium falciparum is exposed to multiple selection pressures. The host environment changes dramatically in severe malaria, but the extent to which the parasite responds to—or is selected by—this environment remains unclear. From previous studies, the parasites that cause severe malaria appear to increase expression of a restricted but poorly defined subset of the PfEMP1 variant, surface antigens. PfEMP1s are major targets of protective immunity. Here, we used RNA sequencing (RNAseq) to analyse gene expression in 44 parasite isolates that caused severe and uncomplicated malaria in Papuan patients. The transcriptomes of 19 parasite isolates associated with severe malaria indicated that these parasites had decreased glycolysis without activation of compensatory pathways; altered chromatin structure and probably transcriptional regulation through decreased histone methylation; reduced surface expression of PfEMP1; and down-regulated expression of multiple chaperone proteins. Our RNAseq also identified novel associations between disease severity and PfEMP1 transcripts, domains, and smaller sequence segments and also confirmed all previously reported associations between expressed PfEMP1 sequences and severe disease. These findings will inform efforts to identify vaccine targets for severe malaria and also indicate how parasites adapt to—or are selected by—the host environment in severe malaria.

show abstract

Section: Methodsmentioning

confidence: 99%

The Plasmodium falciparum transcriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen–encoding var genes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…50% parasitemia. Infected blood was passed through CF-11 cellulose (Whatman) to remove the white blood cells (24). The collected RBC were washed and trophozoite-rich-infected RBC was isolated as described previously (35).…”

Section: Methodsmentioning

confidence: 99%

Antiplasmodial Activity of [(Aryl)arylsulfanylmethyl]Pyridine

Kumar

Das

Dey

et al. 2008

Antimicrob Agents Chemother

View full text Add to dashboard Cite

A series of [(aryl)arylsufanylmethyl]pyridines (AASMP) have been synthesized. These compounds inhibited hemozoin formation, formed complexes (K D ‫؍‬ 12 to 20 M) with free heme (ferriprotoporphyrin IX) at a pH close to the pH of the parasite food vacuole, and exhibited antimalarial activity in vitro. The inhibition of hemozoin formation may develop oxidative stress in Plasmodium falciparum due to the accumulation of free heme. Interestingly, AASMP developed oxidative stress in the parasite, as evident from the decreased level of glutathione and increased formation of lipid peroxide, H 2 O 2 , and hydroxyl radical (·OH) in P. falciparum. AASMP also caused mitochondrial dysfunction by decreasing mitochondrial potential (⌬⌿m) in malaria parasite, as measured by both flow cytometry and fluorescence microscopy. Furthermore, the generation of ·OH may be mainly responsible for the antimalarial effect of AASMP since ·OH scavengers such as mannitol, as well as spin trap ␣-phenyl-n-tertbutylnitrone, significantly protected P. falciparum from AASMP-mediated growth inhibition. Cytotoxicity testing of the active compounds showed selective activity against malaria parasite with selectivity indices greater than 100. AASMP also exhibited profound antimalarial activity in vivo against chloroquine resistant P. yoelii. Thus, AASMP represents a novel class of antimalarial.

show abstract

“…Heparinized red blood cells (RBCs) were washed twice in phosphate-buffered saline (PBS), pH 7.2. The methods of Goldman et al (1992) with a slight modification were used to remove white blood cells from RBCs, which were then passed through a two-layered column composed of 150-210-μm glass beads (Polysciences, Inc., Warrington, PA, USA) and CF 11 cellulose (Whatman International Ltd., Maidstone, UK). After washing twice in PBS, the packed RBCs were diluted threefold and lysed with an equal volume of 0.1 % saponin in PBS.…”

Section: Preparation Of Malarial Antigensmentioning

confidence: 99%

The course of a primary infection of Plasmodium yoelii 17XL in both 129S1 and IFN-γ receptor-deficient mice

2012

View full text Add to dashboard Cite

In the present study, we found that 129S1 mice are resistant to the infection with Plasmodium yoelii 17XL, which is highly virulent and causes lethal infection in various strains of mice. In contrast, IFN-γ receptor-deficient (IFN-γR(-/-)) mice on the 129S1 background were much more susceptible than 129S1 mice with intraperitoneal infection with 1 × 10(5) parasitized erythrocytes. The mortality in 129S1 and IFN-γR(-/-) mice was 11.6 and 79.4 %, respectively. Following inoculation of the parasites, both 129S1 and IFN-γR(-/-) mice showed a progressive increase in parasitemia. Growth rate of malaria parasites at the early stages of infection in the IFN-γR(-/-) mice was faster than that in 129S1 mice, and this difference in growth rate might cause the earlier death of IFN-γR(-/-) host from day 8 of infection than that of 129S1. In surviving mice of both strains, however, malaria parasites in their bloodstream began to decrease in number right after a peak of parasitemia and were not detectable by a microscopic examination during the observation period. Next, we investigated the cytokine and antibody production in 129S1 and IFN-γR(-/-) mice during infection. An analysis of cytokines showed that serum IFN-γ and IL-4 levels elevated significantly from day 1 and day 4 of infection, respectively, in both 129S1 and IFN-γR(-/-) mice when compared with the levels from the uninfected controls. Following the infection, significantly higher levels of malaria-specific IgG1 and IgG2a antibodies in the infected 129S1 mice were detected from day 15, and these elevations were coincident with the decrease of parasitemia. On the other hand, the levels of malaria-specific antibodies in IFN-γR(-/-) mice had a tendency to elevate on day 21 but did not reach statistical significance. The present data indicate that IFN-γR plays an essential role in mediating the early immune mechanisms induced by the infection of erythrocytic stages of P. yoelii 17XL parasite, leading to host survival.

show abstract

Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings

Cited by 11 publications

References 0 publications

The Plasmodium falciparum transcriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen–encoding var genes

The Plasmodium falciparum transcriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen–encoding var genes

Antiplasmodial Activity of [(Aryl)arylsulfanylmethyl]Pyridine

The course of a primary infection of Plasmodium yoelii 17XL in both 129S1 and IFN-γ receptor-deficient mice

Contact Info

Product

Resources

About