Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis

Nunes, Marise P.; Sobral, Ana Cristina Lira; Coutinho, S.

doi:10.1590/s0074-02761992000400007

Cited by 7 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…How could the expression of Sn influence the outcome of Chagas disease? One of the preferential sites of acute infection of T. cruzi is the spleen (Lima et al 2001) and lymph nodes (Nunes et al 1992). The heart is also an organ that has Sn positive macrophages (Croc- Fig.…”

Section: Discussionmentioning

confidence: 99%

Increased association of Trypanosoma cruzi with sialoadhesin positive mice macrophages

et al. 2005

View full text Add to dashboard Cite

Trypanosoma cruzi is a parasite with large amounts of sialic acid (SA) residues exposed at its surface that seems to be involved in macrophages infection. Some macrophages, present in T. cruzi infected tissues, expresses sialoadhesin (Sn), a receptor that recognizes SA. Thus, the involvement of Sn in the association of T. cruzi to macrophages was investigated. Sn was induced in mice peritoneal macrophages by homologous serum (HS) cultivation. Epimastigotes and trypomastigotes associated more to HS cultured macrophages than to fetal bovine serum (FBS). Blocking of Sn with antibodies reduced the association of trypomastigotes to similar level as for FBS cultured macrophages. Desialylation reduced the association of parasites to HS cultured macrophages indicating the Sn importance. Furthermore, the entrance mechanism of trypomastigotes to Sn positive macrophages has a phagocytic nature as demonstrated by scanning electron microscopy and cytochalasin D treatment. Sn positive macrophages may important in the initial trypomastigote infection, thus in the establishment of Chagas disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Increased association of Trypanosoma cruzi with sialoadhesin positive mice macrophages

et al. 2005

View full text Add to dashboard Cite

show abstract

“…However, this method is not capable of detecting small numbers of parasites in tissue. Other methods have been described: the use of limiting dilution assay for quantification of T. cruzi (Nunes et al 1992), karyometry study (Sala et al 2008), histopathology, immunohistochemistry (IHC), and polymerase chain reaction (PCR) assays (Guarner et al 2007). However, all these methods are laborious because they require a subsequent step in the manipulation of the parasite.…”

Section: Resultsmentioning

confidence: 99%

New method for quantification of Trypanosoma cruzi in animal’s tissue in the chronic phase of experimental Chagas' disease

et al. 2010

View full text Add to dashboard Cite

We developed a new method for the quantification of parasites in tissue. Trypanosoma cruzi strain CL parasites were genetically engineered to express the Escherichia coli beta-galactosidase gene, lacZ and this enzyme is able to catalyze a colorimetric reaction with chlorophenol red beta-D: galactopyranoside (CPRG) as the substrate. The animals were infected with clone CL Brener strain B5 of T. cruzi and treated with benznidazole in order to verify the reduction in the number of parasites in tissue study by quantifying the enzyme beta-galactosidase. The assay demonstrates a reduction in the number of parasites in the groups treated. Thus, this test can be used to test other substances with the aim of verifying the effectiveness in the chronic phase of experimental Chagas' disease.

show abstract

“…Together, these data argue that during T. cruzi infection, iNKT cells and NK cells do not contribute to the liver damage. Rather, the liver damage might be caused by parasites as they invade and subsequently destroy hepatocytes (43,44). Furthermore, the serum GPT levels during the infection revealed an initial peak at about day 7 of the infection, followed by a larger second peak at day 14 of the infection.…”

Section: Discussionmentioning

confidence: 99%

NK cell activation and protection occur independently of natural killer T cells during Trypanosoma cruzi infection

Duthie

Kahn

2005

International Immunology

View full text Add to dashboard Cite

Natural killer T (NKT) cells regulate aspects of pro-inflammatory and anti-inflammatory responses and contribute to the control of infections and chronic inflammatory diseases. During Trypanosoma cruzi infection both NKT cells and NK cells are critical to the protective response. How NKT cells interact and possibly regulate NK cells during infections remains uncertain. In vivo studies have demonstrated that specific activation of NKT cells with alpha-galactosylceramide (alpha-GalCer) leads to NK cell activation. These results suggest that during some infections activated NKT cells might regulate NK cell activation and functions. Therefore, using gene-deficient mice that lack NKT cells and antibody-treated mice that lack NK cells, we investigated the interactions of NKT cells and NK cells during experimental T. cruzi infection. We report here that during acute T. cruzi infection spleen and liver NK cell activation and cytolytic activity occur independently of NKT cells. Moreover, NK cell protection occurs independently of NKT cells. In contrast to these results that fail to demonstrate an interdependence, at day 4 of infection the number of liver NK cells is controlled by NKT cells. Thus, during T. cruzi infection, regulation of the number of liver NK cells requires NKT cells, but the activation of NK cells and protection by NK cells does not. The data presented here argue that during infections NK cell activation and protection occur independently of NKT cells.

show abstract

Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis

Cited by 7 publications

References 0 publications

Increased association of Trypanosoma cruzi with sialoadhesin positive mice macrophages

Increased association of Trypanosoma cruzi with sialoadhesin positive mice macrophages

New method for quantification of Trypanosoma cruzi in animal’s tissue in the chronic phase of experimental Chagas' disease

NK cell activation and protection occur independently of natural killer T cells during Trypanosoma cruzi infection

Contact Info

Product

Resources

About