A Brazilian hepatitis A virus isolated and adapted in primate and primate cell line as a chance for the development of a vaccine

Gaspar, Ana; Vitral, Cláudia Lamarca; Marchevsky, Renato Sérgio; Yoshida, Clara Fumiko Tachibana; Schatzmayr, Hermann G.

doi:10.1590/s0074-02761992000300020

Cited by 8 publications

(6 citation statements)

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“…Of these individuals, 9 (45%) were female. Acute hepatitis A cases were defined as follows: (1) by the use of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for anti-HAV IgM (Abbott, USA); (2) by the observation of clinical features of acute-onset hepatitis within 2-8 weeks in a previously healthy individual; and (3) by the observation of aminotransferase levels that were at least 10-fold higher than the upper limit of normal [21][22][23][24]. Patients exhibiting co-infection with other forms of viral hepatitis were not included.…”

Section: Acute Hepatitis a Patientsmentioning

confidence: 99%

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A single dose of inactivated hepatitis A vaccine promotes HAV-specific memory cellular response similar to that induced by a natural infection

Melgaço

Morgado

Santiago³

et al. 2015

Vaccine

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Based on current studies on the effects of single dose vaccines on antibody production, Latin American countries have adopted a single dose vaccine program. However, no data are available on the activation of cellular response to a single dose of hepatitis A. Our study investigated the functional reactivity of the memory cell phenotype after hepatitis A virus (HAV) stimulation through administration of the first or second dose of HAV vaccine and compared the response to that of a baseline group to an initial natural infection. Proliferation assays showed that the first vaccine dose induced HAV-specific cellular response; this response was similar to that induced by a second dose or an initial natural infection. Thus, from the first dose to the second dose, increase in the frequencies of classical memory B cells, TCD8 cells, and central memory TCD4 and TCD8 cells were observed. Regarding cytokine production, increased IL-6, IL-10, TNF, and IFNγ levels were observed after vaccination. Our findings suggest that a single dose of HAV vaccine promotes HAV-specific memory cell response similar to that induced by a natural infection. The HAV-specific T cell immunity induced by primary vaccination persisted independently of the protective plasma antibody level. In addition, our results suggest that a single dose immunization system could serve as an alternative strategy for the prevention of hepatitis A in developing countries.

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Section: Acute Hepatitis a Patientsmentioning

confidence: 99%

“…Non-CFSE-labeled cells were used as a negative control. The HAF-203 HAV strain was propagated in FRhK-4 cells [22]. The virus suspension was filtered through a 0.22 m filter (Merck Millipore, USA) and used for polyclonal HAV antigen-specific proliferation (viral titer of 10 6 HAV RNA copies/mL).…”

Section: Antigen-specific Proliferation Assaymentioning

confidence: 99%

A single dose of inactivated hepatitis A vaccine promotes HAV-specific memory cellular response similar to that induced by a natural infection

Melgaço

Morgado

Santiago³

et al. 2015

Vaccine

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“…But their use in experimental research is severely restricted as they are endangered species, controversial with animal rights advocates, and due to the high costs involved [ 63 ]. During the past three decades, different strains of HAV have been adapted in primates and primate cells in vitro, including C. jacchus [ 65 , 66 ]. Marmosets were suitable to model HAV infection because human HAV could infect them as shown in an earlier study.…”

Section: Callithrix Jacchus Models Of Human LIVmentioning

confidence: 99%

Utility of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells in Liver Disease Modeling, Tissue Engineering and Drug Metabolism

Aravalli

Steer

2020

Genes

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The incidence of liver disease is increasing significantly worldwide and, as a result, there is a pressing need to develop new technologies and applications for end-stage liver diseases. For many of them, orthotopic liver transplantation is the only viable therapeutic option. Stem cells that are capable of differentiating into all liver cell types and could closely mimic human liver disease are extremely valuable for disease modeling, tissue regeneration and repair, and for drug metabolism studies to develop novel therapeutic treatments. Despite the extensive research efforts, positive results from rodent models have not translated meaningfully into realistic preclinical models and therapies. The common marmoset Callithrix jacchus has emerged as a viable non-human primate model to study various human diseases because of its distinct features and close physiologic, genetic and metabolic similarities to humans. C. jacchus embryonic stem cells (cjESC) and recently generated cjESC-derived hepatocyte-like cells (cjESC-HLCs) could fill the gaps in disease modeling, liver regeneration and metabolic studies. They are extremely useful for cell therapy to regenerate and repair damaged liver tissues in vivo as they could efficiently engraft into the liver parenchyma. For in vitro studies, they would be advantageous for drug design and metabolism in developing novel drugs and cell-based therapies. Specifically, they express both phase I and II metabolic enzymes that share similar substrate specificities, inhibition and induction characteristics, and drug metabolism as their human counterparts. In addition, cjESCs and cjESC-HLCs are advantageous for investigations on emerging research areas, including blastocyst complementation to generate entire livers, and bioengineering of discarded livers to regenerate whole livers for transplantation.

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“…O Brasil é considerado um país de alta endemicidade para o vírus A, pois dados epidemiológicos mostram que aproximadamente 90% da população adulta tem anticorpo anti-HVA total e que a maioria das crianças já é imune ao vírus A aos 10 anos de idade 10,11 . Entretanto, devido as enormes variações socioeconômicas no Brasil, há uma grande diversidade na prevalência de infecção pelo vírus A.…”

Section: Epidemiologiaunclassified

Viral hepatitis: update

Ferreira

Silveira²

1997

J Pediatr (Rio J)

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Objective: The authors present an update related to different types of viral hepatitis in infancy. The clinic-laboratorial and special outcome aspects are presented. Methods: The most important articles about viral hepatitis were selected through Medline. The epidemiologic and clinical characteristics related to the subject are reported with emphasis on Brazilian data. Results: This review analyzes the diseases caused by agents that have in common the viral origin and the hepatotropism, but the hepatitis are different particularly in those aspects related to outcome and prognosis. The B virus, for example, may be related to healthy asymptomatic carrier to acute hepatitis, chronic disease, cirrhosis and hepatocellular carcinoma. To date the known viruses are six: A, B, C, D, E and G. Conclusions: Viral hepatitis is a disease caused by one of the 6 different hepatotropic viruses which originate a wide range of clinical presentations. The viral distribution in Brazil is irregular; in some regions the B virus is prevalent, in others it is rare. The viral infections are the most important e the most frequent causes of liver disease in Brazilian children. The present review is to update viral hepatitis in infancy.

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A Brazilian hepatitis A virus isolated and adapted in primate and primate cell line as a chance for the development of a vaccine

Cited by 8 publications

References 0 publications

A single dose of inactivated hepatitis A vaccine promotes HAV-specific memory cellular response similar to that induced by a natural infection

A single dose of inactivated hepatitis A vaccine promotes HAV-specific memory cellular response similar to that induced by a natural infection

Utility of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells in Liver Disease Modeling, Tissue Engineering and Drug Metabolism

Viral hepatitis: update

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