1992
DOI: 10.1590/s0074-02761992000200012
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Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens

Abstract: The present report describes an alternative method for in vitro detection of HIV-1-specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood sampl… Show more

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Cited by 8 publications
(5 citation statements)
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“…Our results shed light on the formation and development of the tropical communities of helminth parasites of freshwater fishes, and give additional support to the idea that the distribution of the species of helminths follows that of their host families [29–31]. When a colonizing fish arrives in a new habitat, it brings its parasites.…”
Section: Discussionsupporting
confidence: 72%
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“…Our results shed light on the formation and development of the tropical communities of helminth parasites of freshwater fishes, and give additional support to the idea that the distribution of the species of helminths follows that of their host families [29–31]. When a colonizing fish arrives in a new habitat, it brings its parasites.…”
Section: Discussionsupporting
confidence: 72%
“…This pattern has been documented in northern temperate fishes [5, 7, 35] as well as in tropical Mexican freshwater fishes [2, 6, 22, 2831, 37]. As a result, host specificity appears to be an important factor in the distribution of parasites, since the distribution of helminths reflects that of the fish families they parasitize [3, 29–31]. This concept is widely accepted; however, few if any investigations have been conducted in order to test this tenet in a given host assemblage.…”
Section: Introductionsupporting
confidence: 56%
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“…The present work also confirms the already described patterns of taxonomical structure of helminth fauna of freshwater fishes of Mexico, as early described by Salgado-Maldonado et al [28], [29]. A dominance by the number of species of trematodes and nematodes is evident for all the fauna, for the helminths from each basin, and for each family of fishes [see [2] and papers cited herein].…”
Section: Discussionsupporting
confidence: 92%