Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells

Castro, Solange L. de; Soeiro, M. N. C.; Meirelles, Maria de Nazareth Leal de

doi:10.1590/s0074-02761992000200007

Cited by 18 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such difference in susceptibility to Et-Blg among the three forms of T. cruzi has been already described in treatments with other drugs (Schlemper et al 1977, De Castro et al 1992, 1993.…”

Section: Discussionsupporting

confidence: 53%

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Dantas

Salomão

Barbosa

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Propolis has shown activity against pathogenic microorganisms that cause diseases in humans and animals. The ethanol (Et-Blg) and acetone (Ket-Blg) extracts from a Bulgarian propolis, with known chemical compositions, presented similar activity against tissue culture-derived amastigotes. The treatment of Trypanosoma cruzi-infected skeletal muscle cells with Et-Blg led to a decrease of infection and of the intracellular proliferation of amastigotes, while damage to the host cell was observed only at concentration 12.5 times higher than those affecting the parasite. Ultrastructural analysis of the effect of both extracts in epimastigotes revealed that the main targets were the mitochondrion and reservosomes. Et-Blg also affected the mitochondrion-kinetoplast complex in trypomastigotes, offering a potential target for chemotherapeutic agents.Key words: propolis -natural products -Trypanosoma cruzi -Chagas disease Chagas disease, caused by the trypanosomatid protozoan Trypanosoma cruzi, is endemic in Latin America. Initially restricted to natural ecotopes, with transmission among small autochthonous mammals by hematophagous Triatominae bugs, the occupation by humans of such ecotopes led to the establishment of the vectors in human dwellings, changing the characteristic of the disease from zoonotic to antropozoonotic. In humans, the acute phase results in 2-8% mortality, specially among children, while in the chronic phase most patients remain asymptomatic and about 30 to 40% of the cases develop symptoms of cardiac or digestive lesions. The World Health Organization estimates that about 18 million people are infected with T. cruzi (WHO 2002). The available drugs for the clinical treatment, the nitroderivatives benznidazole and nifurtimox, are unsatisfactory. They present variable healing effects during the acute phase according to the geographical region and require long term treatment, besides presenting frequent toxic side effects and limited efficacy in the chronic stage (reviewed in Coura & De Castro 2001).In this context, there is an intense search for new synthetic compounds and natural products for treatment of Chagas disease. Thus, our group has been engaged in experimental chemotherapy studies using propolis (Higashi & De Castro 1994, De Castro & Higashi 1995, Marcucci et al. 2001, Prytzyk et al. 2003, Paulino et al. 2003, Salomão et al. 2004). Propolis, a bee product collected from different plant exudates, is a complex mixture containing known bioactive constituents, such as flavonoids, phenolic acids and esters, terpenes, sesquiterpenes, aromatic aldehydes, and alcohols (Marcucci 1995). In temperate zones, bud exudates from poplar trees (Populus spp.) are the main source of flavonoid-rich propolis, which are rich in flavonoids (reviewed in Bankova et al. 2000).During the last decades, an increasing number of studies on the chemical composition, biological activity, and therapeutic uses of propolis has been published (reviewed in De Castro 2001). In recent studies with a Bulgarian propolis sample, we ...

show abstract

“…Such difference in susceptibility to Et-Blg among the three forms of T. cruzi has been already described in treatments with other drugs (Schlemper et al 1977, De Castro et al 1992, 1993.…”

Section: Discussionsupporting

confidence: 53%

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Dantas

Salomão

Barbosa

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Effect on epimastigote proliferation Five-day-old cultures of epimastigotes were collected, centrifuged at 165×g for 1 min (Excelsa 2, Fanen, Brazil) (De Castro et al 1992) and resuspended in LIT to a concentration of 1×10 7 parasites/mL. This suspension (500 μL) was added to the same volume of the MCC or AAA solutions, previously prepared in LIT at twice the desired respective concentration.…”

Section: Methodsmentioning

confidence: 99%

Croton cajucara crude extract and isolated terpenes: activity on Trypanosoma cruzi

et al. 2010

View full text Add to dashboard Cite

Croton cajucara is a plant found in the Amazon region and is known for its medicinal properties. The effects of the methanolic extract of the stem bark of C. cajucara (MCC) and of the isolated terpenes, trans-dehydrocrotonin (t-DCTN) and acetyl aleuritolic acid (AAA), were investigated using four isolates of Trypanosoma cruzi. In assays with trypomastigotes, the extract was more active than the isolated compounds, presenting IC(50) in the range of 10 to 50 μg/mL. The trypanocidal effect of MCC, AAA and benznidazole was significantly higher in the GLT291 and C45 strains, which were recently isolated from wild animals. MCC and AAA caused a dose-dependent inhibition of epimastigote proliferation. In assays using intracellular amastigotes, AAA and MCC reduced the percent of infection and the endocytic index after 96 h of treatment, at concentrations that were non-toxic to the host cells. MCC inhibited the trypanothione reductase pathway in both epimastigotes and trypomastigotes of all the subpopulations. The absence of AAA activity on the trypanothione reductase pathway in epimastigotes of Dm28c suggests heterogeneity of the biochemical profile between this clone and the three strains. Epimastigotes and trypomastigotes (GLT291) were treated for 24 h with MCC or AAA, and both induced alterations of the plasma membrane, while AAA-treated epimastigotes also displayed mitochondrial damage.

show abstract

“…0.25% − 1.42% flavonoids have been identified in different groundnut shell samples, according to their origins, varieties and maturities (Han et al, 2006). In most reports, the biological or pharmacological activity was associated with phenolic compounds, mainly with flavonoids (Burdock, 1998;De Castro et al, 1992) . Flavonoids from peanut hull are potent antioxidants, free radical scavengers and metal chelators: they inhibit lipid peroxidation and exhibit various physiological activities, including antihypertensive and anti-inflammatory activities (Wang, 2000;Harborne et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Study on Ultrasonic Extraction Technology and Oxidation Resistance of Total Flavonoids from Peanut Hull

Yang

Sun

et al. 2011

FSTR

View full text Add to dashboard Cite

show abstract

Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells

Cited by 18 publications

References 0 publications

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

The effect of Bulgarian propolis against Trypanosoma cruzi and during its interaction with host cells

Croton cajucara crude extract and isolated terpenes: activity on Trypanosoma cruzi

Study on Ultrasonic Extraction Technology and Oxidation Resistance of Total Flavonoids from Peanut Hull

Contact Info

Product

Resources

About