On the presence of elastic microfibrils in liver granuloma of murine schistosomiasis mansoni

Cotta-Pereira, Gerson; Oliveira, Denise N.; Wajsenzoni, I. J. R.; Lenzi, J. A.

doi:10.1590/s0074-02761991000700028

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“…The mice were killed at the following times after infection: 35, 40, 45, 60, 70, 90, 120 and 180 days. Liver fragments were homogenized for 2 min in a blender and the suspensions were filtered through grids to select isolated granulomas of two different sizes: smaller and larger than 300 µm (12). Purified granulomas and liver fragments were fixed in Carson s modified Millonig s phosphatebuffered formalin, pH 7.4 (13), and embedded in paraffin.…”

Section: Introductionmentioning

confidence: 99%

Collagen arrangement in hepatic granuloma in mice infected with Schistosoma mansoni: dependence on fiber radiation centers

Lenzi

Kimmel

Schechtman

et al. 1999

Braz J Med Biol Res

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The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers probably depends on the fibroblastic or myofibroblastic organization of stress fibers. Gradually, the loose unstable lattice of the exudative-productive stage transforms into a highly packed and stable architecture as a result of progressive compactness. The three-dimensional architecture of granulomas provides increased tissue integrity, efficient distribution of soluble compounds and a haptotactic background to the cells.

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Section: Introductionmentioning

confidence: 99%