Interaction of TPA-treated trichomonads with fibronectin-coated substrata

Bonilha, Vera L.; Filho, Fernando Costa Silva

doi:10.1590/s0074-02761991000700025

Cited by 2 publications

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“…In the case of Tritrichomonas foetus, which is a protozoan found in the urogenital tract of cattle, there is very little information on basic aspects of the process of its interaction with cells from the host, although there are reports on the presence of receptors for extracellular-matrix proteins (Bonilha and Silva Filho 1990;). It has previously been shown that T. foetus, but not T. vaginalis, displays a high level of hemagglutinating activity and that during this process a focal adhesion, with changes in the structural organization of the plasma membrane, takes place (Silva et al 1996).This observation suggests that on its surface, T. foetus exposes molecules with an adhesin-like activity.…”

Section: Introductionmentioning

confidence: 99%

Identification and localization of an adhesin on the surface of Tritrichomonas foetus

Silva

Filho

Souza

1999

Parasitology Research

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Tritrichomonas foetus is a mucosal parasite of the urogenital-vaginal tract of cattle that strongly adheres to erythrocytes, which suggests that it presents an adhesin that recognizes red blood cells from different animal species and blood groups. In the present report we describe a cell-fractionation method for obtainment of a membrane fraction of T. foetus, which adhered to red blood cells. The T. foetus adhesin was obtained after parasite lysis and fractionation followed by ultracentrifugation, whereby a 100,000-g pellet fraction showed a strong hemagglutinating activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of this fraction, of erythrocyte ghosts, and of ghosts allowed to interact with the parasite membrane fraction revealed the presence of a 100-kDa protein as the putative adhesin. Polyclonal antibodies obtained in rabbits immunized with this protein recognized proteins of 100 and 90 kDa as determined by immunoblotting. Confocal laser scanning microscopy and transmission electron microscopy of cells incubated first in the presence of the antibody and subsequently in the presence of fluorescein- or gold-labeled goat anti-rabbit IgG showed labeling of the protozoan surface as well as of some cytoplasmic vesicles.

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Section: Introductionmentioning

confidence: 99%

Identification and localization of an adhesin on the surface of Tritrichomonas foetus

Silva

Filho

Souza

1999

Parasitology Research

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The involvement of terminal carbohydrates of the mammalian cell surface in the cytoadhesion of trichomonads

et al. 1995

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In the present study the parental cells and glycosylation mutants of Chinese hamster ovary (CHO) cells were used to analyze the influence of surface carbohydrates on the cytoadhesion of trichomonads. Trichomonas vaginalis and Tritrichomonas foetus were allowed to interact with host cells for 2 h at 37 degrees C. Alternatively, CHO cells were treated with 10 mM periodate prior to the assays. Both trichomonads adhered to all CHO cell clones tested. A remarkable difference could be observed between the cytoadhesion of T. vaginalis and T. foetus. Sialic acid residues present on the surface of CHO cells may favor the cytoadhesion of T. foetus while hampering that of T. vaginalis. The specificity of the parasite cytoadhesion was further investigated. Sialic acid, mannose, and galactose as well as mannose, galactose, and N-acetylglucosamine added to the interaction medium at 50, 100, and 200 mM were capable of significantly inhibiting the cytoadhesion of each trichomonad species. Periodate treatment of target cells also induced decreases in the cytoadhesion of the trichomonads. These results strongly suggest an important role for host-cell surface glycoconjugates during the cytoadhesion of trichomonads. In addition, they also point out the presence of "lectin-like" molecules on the surface of both T. vaginalis and T. foetus.

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Interaction of TPA-treated trichomonads with fibronectin-coated substrata

Cited by 2 publications

References 0 publications

Identification and localization of an adhesin on the surface of Tritrichomonas foetus

Identification and localization of an adhesin on the surface of Tritrichomonas foetus

The involvement of terminal carbohydrates of the mammalian cell surface in the cytoadhesion of trichomonads

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