Proteinase inhibitors in Brazilian leguminosae

Abstract

Serine proteinase inhibitors, in the seeds of several Leguminosae from the Pantanal region (West Brazil), were studied using bovine trypsin, a digestive enzyme, Factor XIIa and human plasma kallikrein, two blood clotting factors. The inhibitors were purified from Enterolobium contortisiliquum (M(r) = 23,000), Torresea cearensis (M(r) = 13,000), Bauhinia pentandra (M(r) = 20,000) and Bauhinia bauhinioides (M(r) = 20,000). E. contortisiliquum inhibitor inactivates all three enzymes, whereas the T. cearensis inhibitor inactivates trypsin and Factor XIIa, but does not affect plasma kallikrein; both Bauhinia inhibitors, on the other hand, inactivate trypsin and plasma kallikrein but only the B. pentandra inhibitor affects Factor XIIa. Ki values were calculated between 10(-7) and 10(-8) M.

State-of-knowledge on Amburana cearensis (Fr. Allem.) A.C. Smith (Leguminosae: Papilionoideae) for genetic conservation in Brazil

“…Seed is used for perfume and soap (Record & Hess, 1972) Medical investigation Sampaio, Oliva, Tanaka, and Sampaio (1991) and Tanaka, Sampaio, Sampaio, and Oliva (1989) reported the inhibition of blood coagulation by factors in extracts from the seed of A. cearensis. Proteinase inhibitors are considered responsible for this effect.…”

Modern strategy for new medicine research: study of pharmacological activities of riachin, a non-cyanogenic cianoglycoside extracted from Bauhinia pentandra

“…This has been used by people for many years, especially for presenting hypoglycemic activity, a reported activity since the 1990s by several authors [1,2,3]. Another species that has shown scientific importance is the Bauhinia pentandra has been shown antiulcer activity4 and acts on the inhibition of coagulation factor XIIa [5,6]. The species Bauhinia pentandra (Bong.)…”

Antibiotic-modifying activity of riachin, a non-cyanogenic cyanoglycoside extracted from Bauhinia pentandra

“…7 – 9 Another species that has shown scientific relevance is Bauhinia pentandra , which has demonstrated antiulcer activity, 10 and has an inhibitory effect on factor XIIa in the coagulation cascade. 11 , 12 …”