Lectin receptors distribution in the surface membrane of Trypanosoma cruzi blood forms collected from mice submitted to specific treatment

Fontes, Gilberto; Romanha, Álvaro J.; Pereira, M. E. S.; Brener, Z.

doi:10.1590/s0074-02761991000300003

Cited by 2 publications

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“…However, no morphological change was observed in organelles from WT parasites obtained from treated animals, as evaluated by electronic microscopy. In addition, earlier studies performed by Fontes et al (1991) demonstrate that BZ-treatment of mice infected with susceptible and naturally resistant T. cruzi strains does not affect the carbohydrate distribution in the membrane of the parasite. Current studies are being performed in our laboratory, in order to define the molecular and structural surface changes observed on the BZ-susceptible T. cruzi after in-vivo treatment with BZ.…”

Section: Discussionmentioning

confidence: 84%

In‐vivo treatment with benznidazole enhances phagocytosis, parasite destruction and cytokine release by macrophages during infection with a drug‐susceptible but not with a derived drug‐resistant Trypanosoma cruzi population

Murta

Ropert²,

Alves

et al. 1999

Parasite Immunology

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To stuck the effect of chemotherapy on parasite-macrophage interaction we used the wild-type Y strain (drug-susceptible) of Trypanosoma cruzi and a drug-resistant parasite population derived from the same strain. Trypomastigotes isolated from untreated infected mice, as well as, 3 h after treatment with BZ were incubated with inflammatory macrophages and used to study phagocytosis, parasite destruction, cytokine release and reactive nitrogen intermediates (RN!) synthesis. Phagocytosis and destruction of the drug-susceptible parasites were significant/v enhanced by drug treatment. These enhancements were accompanied by an increase in cytokines [interleukin (IL)-12 and tumour necrosis factor (TNF)alpha] and RNI release by murine inflammatory macrophages primed with IFN-gamma. In contrast, BZ treatment of mice infected with drug-resistant T. cruzi population showed no effect whatsoever. The synthesis of IFN-gamma and RNI by splenocytes of mice infected with either susceptible and drug-resistant parasite populations, before and after treatment with BZ were also studied. On/v the splenocytes from mice infected with the drug-susceptible parasites treated with BZ produced high levels of IFN-gamma and RNI. Our findings indicate that BZ acts on the drug-susceptible T. cruzi parasites by enhancing the phagocytosis and the production of cytokines and RN!, thus, favouring the destruction of the intracellular parasites by the cellular compartment of the immune system.

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Section: Discussionmentioning

confidence: 84%