Use of Trypanosoma cruzi defined proteins for diagnosis-multicentre trial: serological and technical aspects

Ao, Luquetti

doi:10.1590/s0074-02761990000400021

Cited by 29 publications

(17 citation statements)

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“…In Latin America, the most commonly tests for screening, and sometimes for confirmation, are the enzyme immunoassays using conventional (crude antigens) or non conventional reagents (mixture of peptides or recombinant antigens) (Luquetti 1990, Ferreira et al 2001, Umezawa et al 2003. The IHA is also used for screening and, on occasions, as a second test for confirmation.…”

Section: Preventionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

474

294

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Section: Preventionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

474

294

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“…The use of purified antigen for the conclusive and reliable diagnosis of Chagas' disease has been emphasized as being of paramount importance among the different issues currently under study concerning this parasitosis [10,11].…”

Section: Introductionmentioning

confidence: 99%

Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6)

Malchiodi

Chiaramonte

Taranto³

et al. 1994

Clinical and Experimental Immunology

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SUMMARY Results of our studies on the reactivity of chagasic and leishmaniasic sera with the purified T. cruzi‐ specific antigen 163B6, as assessed by ELISA, and with complex antigenic mixtures from T. cruzi and Leishmania mexicana, by immunoblotting. are presented here. Our objective was to identify the antigens responsible for the exhibited cross‐reactivity between trypanosomiasis and leishmaniasis, and to find a specific reactivity pattern corresponding to each parasitosis. In spite of the high cross‐reactivity observed with the immunoblotting, the use of 7.5% A‐B gels made it possible to identify a characteristic pattern for each parasitosis, that could be distinguished by the naked eye. The characteristic pattern corresponding to chagasic patients was ascribed to reactivity with T, cruzi bands of mol. wts 131, 125, 116, 111, 51‐45 and 43 kD, that were not recognized by leishmaniasic sera. Trypanosoma cruzi antigens of mol. wts 85, 81, 70, 65 60, 37 and 32kD were considered as crossing antigens, since they were recognized by leishmaniasis sera. With L. mexicana, most of the chagasic patients presented reaction with antigen of mol. wts 124, 107. 92, 59 and 32kD, while bands of mol. wts 155, 140. 73. 56 and 48kD were recognized only by leishmaniasic sera. In this study we found 12 out of 45 sera of patients with leishmaniasis, from a region endemic for both parasitoses, which exhibited a pattern of bands very similar to those corresponding to chagasic individuals, strongly suggesting a mixed infection. This hypothesis was verified by using a purified specific antigen of T. cruzi, Ag163B6, which would be the major cysteine proteinase of this specie (cruzipain). By HLISA, these 12 sera showed a positive reaction with this purified antigen, as those of chagasic patients, thus leading to the confirmation of the presence of a mixed infection.

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“…The World Health Organization has long emphasized the need for defined antigens to improve serodiagnosis of Chagas' disease. In an attempt to solve this problem, several research groups have used recombinant and/or synthetic and biochemically purified antigens (1,7,12,(14)(15)(16)19). In order to be useful, these antigens must meet the following criteria: (i) they should be present in T. cruzi isolates from different areas of endemicity and absent in other infectious disease agents; (ii) they should be highly immunogenic in populations with different immunogenetic backgrounds, regardless of the clinical phase of Chagas' disease; and (iii) they should be stable and easily amenable to quality control tests, to guarantee reproducibility (18,24).…”

mentioning

confidence: 99%

Excretory-Secretory Antigens of Trypanosoma cruzi Are Potentially Useful for Serodiagnosis of Chronic Chagas' Disease

Nakazawa

Rosa

Pereira

et al. 2001

Clin Diagn Lab Immunol

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Use of Trypanosoma cruzi defined proteins for diagnosis-multicentre trial: serological and technical aspects

Cited by 29 publications

References 0 publications

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6)

Excretory-Secretory Antigens of Trypanosoma cruzi Are Potentially Useful for Serodiagnosis of Chronic Chagas' Disease

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