Molecular biology of Anticarsia gemmatalis baculovirus

Maruniak, James E.

doi:10.1590/s0074-02761989000700020

Cited by 15 publications

(12 citation statements)

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“…In our study, the enzymes BamHI, BgllI, HindlII, EcoR], and PstI were found to cleave the AgMNPV-CL4-3A1 genome into 4,5, 18, 18, and 21 fragments, respectively. These fragment numbers are different from those reported by Johnson and Maruniak (4) for BamHI (four fragments), BgllI, (seven fragments), EcoRl (10 fragments), and HindlII (24 fragments) restriction profiles of AgMNPV-2 and from what Maruniak (7) reported in six other plaqued, purified AgMNPV isolates (mean number HindlIl fragments 24.8; mean number PstI fragments 23.7). Inherent variation in the number of restriction fragments and fragment sizes could probably HOST RANGE OF VIRUS tN LEPIDOPTERANS 8 3…”

Section: Hind III Hind Iii Ecori Bamhi Bqtl_lcontrasting

confidence: 89%

In vitro and in vivo host range of Anticarsia Gemmatalis multiple nuclear polyhedrosis virus

Grasela

McIntosh

1998

In Vitro Cell.Dev.Biol.-Animal

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A clone of the wild type (wt) Anticarsia gemmatalis multiple nuclear polyhedrosis virus AgMNPV, derived from a geographical isolate (Hondrina, Brazil) and designated AgMNPV-CL4-3A1, was used to determine the host range of this virus in six established lepidopteran cell lines: Anticarsia gemmatalis (BCIRL-AG-AM1), Helicoverpa zea (BCIRL-HZ-AM1), Heliothis virescens (BCIRL-HV-AM1), Helicoverpa armigera (BCIRL-HA-AM1), Trichoplusia ni (TN-CL1), Bombyx mori (BMN), and a coleopteran cell line Anthonomus grandis (BRL-AG-1). In addition, the in vivo host range of this clone was also assayed in larvae of Helicoverpa zea, Heliothis virescens, Trichoplusia ni, and the homologous species Anticarsia gemmatalis by probit analysis. On the basis of temporal studies of TCID50 values, BCIRL-HV-AM1 cells gave the highest extracellular virus (ECV) titer (9.7 x 10(6) TCID50/ml) followed by BCIRL-HA-AM1 cells (8.3 x 10(5) TCID50/ml) and BCIRL-AG-AM1 cells (3.2 x 10(5) TCID50/ml). In addition, a low ECV titer of 1.37 x 10(3) TCID50/ml was detected from TN-CL1 cells 96 h postinoculation, while BRL-AG-1, BMN, and BCIRL-HZ-AM1 cells were nonpermissive to AgMNPV-CL4-3A1 on the basis of TCID50 results. AgMNPV-CL4-3A1 and the wild type AgMNPV had similar restriction profiles that were different from wild type AcMNPV. The LC50 values were 96.9, 564.6, 733.3, and 1.1 x 10(4) occlusion bodies/cm2 of diet for A. gemmatalis, Helicoverpa zea, Heliothis virescens, and T. ni, respectively.

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Section: Hind III Hind Iii Ecori Bamhi Bqtl_lcontrasting

confidence: 89%

In vitro and in vivo host range of Anticarsia Gemmatalis multiple nuclear polyhedrosis virus

Grasela

McIntosh

1998

In Vitro Cell.Dev.Biol.-Animal

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“…The virus inoculum consisted of ten distinct genotypic variants (1b, 2a, 2b, 7a, 9a, 10b, 15a, 15b, 18a,and 33b) obtained from the AgMNPV-Ds20 isolate by plaque purification in IPBL-SF-21 cells (Maruniak, 1989). Polyhedral inclusion bodies (PIBs) obtained from IPLB-SF-21 cells were purified by differential centrifugation and counted using a hemacytometer (American Optical, Buffalo, NY).…”

Section: Methodsmentioning

confidence: 99%

“…Unable to estimate LD 50 values because less than 50% mortality was obtained with the highest tested dosage (10 5 PIBs/larva). and selected based on differences found in the PstI and BamHI restriction profiles (Maruniak, 1989). An in vitro analysis of these variants showed no significant differences in the biological activity against Spodoptera frugiperda (IPLB-SF-21) and A. gemmatalis (UFL-AG-286) cells (Maruniak, 1989).…”

Section: Introductionmentioning

confidence: 99%

Comparative susceptibility of two different hosts to genotypic variants of the Anticarsia gemmatalis nuclear polyhedrosis virus

Ribeiro

Pavan

Muotri

1997

Entomologia Exp Applicata

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The susceptibility of third instar larvae of Anticarsia gemmatalis (Lepidoptera: Noctuidae) and Diatraea saccharalis (Lepidoptera: Pyralidae) to ten distinct plaque purified genotypic variants of a selected isolate of the Anticarsia gemmatalis multiple‐embedded nuclear polyhedrosis virus (AgMNPV), was compared. Despite the fact that this isolate, AgMNPV‐Ds20, represents a wild strain of the AgMNPV selected for higher virulence to D. saccharalis, an alternate host, most of the variants are much more virulent to the original host Anticarsia than to Diatraea. Bioassays have shown an over one hundred‐fold variation in LD50 values ranging from 1700 polyhedron inclusion bodies (PIBs) to more than 200 000 PIBs/larva. The PIB production in infected larvae increased with the pathogenicity of the variant to the host, showing an average ten‐fold reduction in Diatraea when compared to Anticarsia for the same variant. The virus particle yield ranged from 6×107 to more than 109 PIBs/g of infected larvae in Diatraea and from 8×108 to more than 1010 PIBs/g of infected Anticarsia larvae. The data show a clear difference of the pathogenicity of the genotypic variants of AgMNPV in vivo both between the original and alternate host and between the individual variants for the same host. These differences found in vivo indicate that monitoring of shifts in variant frequency of wild and laboratory‐propagated viral isolates in these highly heterogeneous populations would help ensure the efficacy of biological control programs.

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“…Studies with genotypic variants plaque-purified from field isolates and host range variants, as well as studies of genomic organization are under way (Maruniak, 1989). AgMNPV replicates in Trichoplusia ni TN368 (Hink, 1970) and Spodoptera frugiperda IPLB-Sf21-AE cells (Vaughn et al, 1977), as well as in the A. gemmatalis UFL-AG-286 embryo-derived cell line (Sieburth & Maruniak, 1988).…”

Section: Introductionmentioning

confidence: 99%

The Anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons

Zanotto

Sampaio

Johnson

et al. 1992

Journal of General Virology

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The genomic region of the Anticarsia gemmatalis multiple nucleocapsid nuclear polyhedrosis virus (AgMNPV) strain 2D encoding the polyhedrin gene was cloned and mapped, and a 2085 bp SphI-Pstl fragment containing the gene was sequenced. The polyhedrin polypeptide of the parental isolate AgMNPV was manually sequenced, and the amino acid sequence obtained agreed with that deduced from the DNA coding region sequence. AgMNPV and Orgyia pseudotsugata MNPV (OpMNPV) are similar in terms of promoter structure and polyhedrin primary sequence, and the polyhedrin gene of both viruses is transcribed in the anti-clockwise direction in relation to their physical maps. The region upstream from the polyhedrin gene of AgMNPV, OpMNPV, Bombyx mori NPV and ,4utographa californica MNPV (AcMNPV) was compared and this showed that the open reading frame (ORF) common to all four viruses (ORF 5) has sequence homology with the AcMNPV 25K gene. The sequences between ORF 5 and the polyhedrin gene were found to be variable among the polyhedrin gene loci compared. Additionally, conserved elements in the promoters of the very late genes encoding polyhedrin and granulin, and those encoding two pl0 proteins were found to share sequence homology and positional similarity with consensus regions in the conserved boxes A and C, responsible for binding transcription factors to eukaryotic 5S ribosomal RNA genes, and to box C of tRNA genes.

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Molecular biology of Anticarsia gemmatalis baculovirus

Cited by 15 publications

References 0 publications

In vitro and in vivo host range of Anticarsia Gemmatalis multiple nuclear polyhedrosis virus

In vitro and in vivo host range of Anticarsia Gemmatalis multiple nuclear polyhedrosis virus

Comparative susceptibility of two different hosts to genotypic variants of the Anticarsia gemmatalis nuclear polyhedrosis virus

The Anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons

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