New facts and trends in bacteriological control of mosquitoes

Barjac, H. de

doi:10.1590/s0074-02761989000700019

Cited by 7 publications

(3 citation statements)

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“…At least 92% of all human proteins contain at least one cysteine residue [26] and thus can be detected using the thiol-labeling maleimide dye [18, 20]. To detect the changes in abundance and S-NO modification levels, each sample was split into two fractions (each fraction containing 100 μ g of protein) [27]. Because some cysteine thiols are hyperreactive that can be oxidized under aerobic conditions in few minutes if without any protection [28], we immediately treated one fraction with copper chelator neocuproine (100 µ M for 1 h) that is shown to prevent S-NO reduction and stabilize S-NO during further processing of samples.…”

Section: Methodsmentioning

confidence: 99%

S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure

Koo

Spratt

Soman

et al. 2016

International Journal of Proteomics

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Nitric oxide (NO) protects the heart against ischemic injury; however, NO- and superoxide-dependent S-nitrosylation (S-NO) of cysteines can affect function of target proteins and play a role in disease outcome. We employed 2D-GE with thiol-labeling FL-maleimide dye and MALDI-TOF MS/MS to capture the quantitative changes in abundance and S-NO proteome of HF patients (versus healthy controls, n = 30/group). We identified 93 differentially abundant (59-increased/34-decreased) and 111 S-NO-modified (63-increased/48-decreased) protein spots, respectively, in HF subjects (versus controls, fold-change | ≥1.5|, p ≤ 0.05). Ingenuity pathway analysis of proteome datasets suggested that the pathways involved in phagocytes' migration, free radical production, and cell death were activated and fatty acid metabolism was decreased in HF subjects. Multivariate adaptive regression splines modeling of datasets identified a panel of proteins that will provide >90% prediction success in classifying HF subjects. Proteomic profiling identified ATP-synthase, thrombospondin-1 (THBS1), and vinculin (VCL) as top differentially abundant and S-NO-modified proteins, and these proteins were verified by Western blotting and ELISA in different set of HF subjects. We conclude that differential abundance and S-NO modification of proteins serve as a mechanism in regulating cell viability and free radical production, and THBS1 and VCL evaluation will potentially be useful in the prediction of heart failure.

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Section: Methodsmentioning

confidence: 99%

S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure

Koo

Spratt

Soman

et al. 2016

International Journal of Proteomics

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“…This study respects the efforts of several investigators against the dangerous use of chemical insecticides for pest control; these chemicals are still widely marketed (Fares, 1996). In the mid 1970s, the World Health Organization (WHO) and other international institutions initiated studies on the development of existing and new biological control agents (de Barjac, 1989). The most popular of these agents are strains of the 'Bacillus thuringiensis'.…”

Section: Introductionmentioning

confidence: 99%

“…Among these Bacillus thuringiensis var. kurstaki, was proven to produce an effective toxin against lepidopteran insects (Tyrell et al, 1981, de Barjac, 1989Singsit et al, 1997). These spore-forming entomopatho-genic bacteria are gram-positive and have a unique ability to produce parasporal-proteinaceous crystalline inclusions during sporulation (Caramori et al, 1991;Sanchis et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Fine Structural Changes in the Ileum of Mice Fed on δ‐Endotoxin‐Treated Potatoes and Transgenic Potatoes

Fares

El-Sayed

1998

Nat. Toxins

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The present work has been designed to study the effect of feeding on transgenic potatoes, which carry the CryI gene of Bacillus thuringiensis var. kurstaki strain HD1, on the light and electron microscopic structure of the mice ileum, in comparison with feeding on potatoes treated with the 'delta-endotoxin' isolated from the same bacterial strain. The microscopic architecture of the enterocytes of the ileum of both groups of mice revealed certain common features such as the appearance of mitochondria with signs of degeneration and disrupted short microvilli at the luminal surface. However, in the group of mice fed on the 'delta-endotoxin', several villi appeared with an abnormally large number of enterocytes (151.8 in control group versus 197 and 155.8 in endotoxin and transgenic-treated groups, respectively). Fifty percent of these cells were hypertrophied and multinucleated. The mean area of enterocyte was significantly increased (105.3 microm(2) in control group versus 165.4 microm(2) and 116.5 microm(2) in endotoxin and transgenic-treated groups, respectively). Several forms of secondary lysosomes or auotophagic vacuoles were recognized in these cells. These changes were confirmed with the scanning electron microscope which revealed a remarkable increase in the topographic contour of enterocytes (23 microm in control group versus 44 microm and 28 microm in endotoxin and transgenic-treated groups, respectively) at the divulged surface of the villi. The basal lamina along the base of the enterocytes was damaged at several foci. Several disrupted microvilli appeared in association with variable-shaped cytoplasmic fragments. Some of these fragments contained endoplasmic reticulum, as well as ring-shaped annulate lamellae. In addition, the Paneth cells were highly activated and contained a large number of secretory granules. These changes may suggest that delta-endotoxin-treated potatoes resulted in the development of hyperplastic cells in the mice ileum. Although mild changes are reported in the structural configuration of the ileum of mice fed on transgenic potatoes, nevertheless, thorough tests of these new types of genetically engineered crops must be made to avoid the risks before marketing.

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Fine Structural Changes in the Ileum of Mice Fed on δ-Endotoxin-Treated Potatoes and Transgenic Potatoes

Fares

El-Sayed

1998

Nat. Toxins

View full text Add to dashboard Cite

The present work has been designed to study the effect of feeding on transgenic potatoes, which carry the CryI gene of Bacillus thuringiensis var. kurstaki strain HD1, on the light and electron microscopic structure of the mice ileum, in comparison with feeding on potatoes treated with the ‘δ‐endotoxin’ isolated from the same bacterial strain. The microscopic architecture of the enterocytes of the ileum of both groups of mice revealed certain common features such as the appearance of mitochondria with signs of degeneration and disrupted short microvilli at the luminal surface. However, in the group of mice fed on the ‘δ‐endotoxin’, several villi appeared with an abnormally large number of enterocytes (151.8 in control group versus 197 and 155.8 in endotoxin and transgenic‐treated groups, respectively). Fifty percent of these cells were hypertrophied and multinucleated. The mean area of enterocyte was significantly increased (105.3 µm2 in control group versus 165.4 µm2 and 116.5 µm2 in endotoxin and transgenic‐treated groups, respectively). Several forms of secondary lysosomes or auotophagic vacuoles were recognized in these cells. These changes were confirmed with the scanning electron microscope which revealed a remarkable increase in the topographic contour of enterocytes (23 µm in control group versus 44 µm and 28 µm in endotoxin and transgenic‐treated groups, respectively) at the divulged surface of the villi. The basal lamina along the base of the enterocytes was damaged at several foci. Several disrupted microvilli appeared in association with variable‐shaped cytoplasmic fragments. Some of these fragments contained endoplasmic reticulum, as well as ring‐shaped annulate lamellae. In addition, the Paneth cells were highly activated and contained a large number of secretory granules. These changes may suggest that δ‐endotoxin‐treated potatoes resulted in the development of hyperplastic cells in the mice ileum. Although mild changes are reported in the structural configuration of the ileum of mice fed on transgenic potatoes, nevertheless, thorough tests of these new types of genetically engineered crops must be made to avoid the risks before marketing. Copyright © 1998 John Wiley & Sons, Ltd.

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New facts and trends in bacteriological control of mosquitoes

Cited by 7 publications

References 0 publications

S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure

S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure

Fine Structural Changes in the Ileum of Mice Fed on δ‐Endotoxin‐Treated Potatoes and Transgenic Potatoes

Fine Structural Changes in the Ileum of Mice Fed on δ-Endotoxin-Treated Potatoes and Transgenic Potatoes

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