Nova concepção para o método de Baermann-Moraes-Coutinho na pesquisa de larvas de nematódeos

Willcox, Henry P. F.; Coura, José Rodrigues

doi:10.1590/s0074-02761989000400015

Cited by 33 publications

(11 citation statements)

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“…In a study conducted in China comparing DS, an ether concentration technique (ECT), Kato-Katz, Koga agar plate method and Baermann method, the best sensitivity was obtained with the Baermann method (all cases were detected by this method) and both ECT and DS failed to identified even a single case [37]. This technique is labor intensive and it is not usually available in clinical parasitology laboratories but there have been several attempts to reduce the cost and to simplify the technique through slight modifications of the Baermann procedure [38], [39], [40]. Another drawback of the technique is that it requires freshly and non-refrigerated stool samples.…”

Section: Resultsmentioning

confidence: 99%

The Laboratory Diagnosis and Follow Up of Strongyloidiasis: A Systematic Review

et al. 2013

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BackgroundStrongyloidiasis is frequently under diagnosed since many infections remain asymptomatic and conventional diagnostic tests based on parasitological examination are not sufficiently sensitive. Serology is useful but is still only available in reference laboratories. The need for improved diagnostic tests in terms of sensitivity and specificity is clear, particularly in immunocompromised patients or candidates to immunosuppressive treatments. This review aims to evaluate both conventional and novel techniques for the diagnosis of strongyloidiasis as well as available cure markers for this parasitic infection.Methodology/Principal FindingsThe search strategy was based on the data-base sources MEDLINE, Cochrane Library Register for systematic review, EmBase, Global Health and LILACS and was limited in the search string to articles published from 1960 to August 2012 and to English, Spanish, French, Portuguese and German languages. Case reports, case series and animal studies were excluded. 2003 potentially relevant citations were selected for retrieval, of which 1649 were selected for review of the abstract. 143 were eligible for final inclusion.ConclusionsSensitivity of microscopic-based techniques is not good enough, particularly in chronic infections. Furthermore, techniques such as Baermann or agar plate culture are cumbersome and time-consuming and several specimens should be collected on different days to improve the detection rate. Serology is a useful tool but it might overestimate the prevalence of disease due to cross-reactivity with other nematode infections and its difficulty distinguishing recent from past (and cured) infections. To evaluate treatment efficacy is still a major concern because direct parasitological methods might overestimate it and the serology has not yet been well evaluated; even if there is a decline in antibody titres after treatment, it is slow and it needs to be done at 6 to 12 months after treatment which can cause a substantial loss to follow-up in a clinical trial.

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Section: Resultsmentioning

confidence: 99%

The Laboratory Diagnosis and Follow Up of Strongyloidiasis: A Systematic Review

et al. 2013

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“…The feces of parasitized R. norvergicus were collected to obtain the larvae by the technique of Baermann (Willcox and Coura, 1989). After processing the fecal samples, specimens of B. glabrata (8-12 mm) at 90 days old on average were exposed individually to approximately 1200 L 1 larvae (Yousif and Lammler, 1977).…”

Section: Infection Of the Snailsmentioning

confidence: 99%

Changes in the reproductive biology of Biomphalaria glabrata experimentally infected with the nematode Angiostrongylus cantonensis

Tunholi-Alves

Tunholi

Lustrino

et al. 2011

Journal of Invertebrate Pathology

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This study showed for the first time changes in the reproductive biology of Biomphalaria glabrata experimentally infected with Angiostrongylus cantonensis. The values of all the parameters analyzed (total number of eggs, number of egg masses, number of eggs/mass, number of eggs/snail, percentage of viable eggs and galactogen content in albumen gland) changed with progressive infection. The results indicate the occurrence of partial parasitic castration of B. glabrata by A. cantonensis larvae, probably in response to the depletion of energy reserves, with no injuries to the gonadal tissues.

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“…The feces of infected S. hispidus were collected and placed in a Baermann apparatus to separate the decanted L1 (Willcox and Coura 1989). After 2 days of fasting, mollusks were individually fed with a fragment of lettuce covered with healthy mice feces and a concentrate of 1,200 L1 (Morera 1973).…”

Section: Mollusk Infectionmentioning

confidence: 99%

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Montresor

Vidigal

Mendonça³

et al. 2008

Parasitol Res

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Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with stage 1 larvae. Obtained stage 3 larvae were orally inoculated in mice, and after 30 days, adult worms and stage 1 larvae were recovered, demonstrating Omalonyx susceptibility and suitability to infection. To define the parasite migration routes, specimens of Omalonyx with 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 2 days, 5 days, 10 days, 12 days, 15 days, 20 days, 21 days, 25 days, 28 days, and 30 days of infection were fixed and serially sectioned. Histological sections were stained with hematoxylin-eosin. The results were compared to those described in S. marginata. Oral and cutaneous infections were noted. After the penetration, larvae were retained, mainly in the fibromuscular tissue, by hemocytes, or they spread to the whole organism through the circulation, following the anatomical structure of the vasculature. The perilarval hemocyte reaction in Omalonyx was more intense until stage 2 larva instar, decreasing in the presence of stage 3 larvae. Differences in some aspects of hemocyte reaction between S. marginata and Omalonyx exemplify interspecific peculiarities in snail response to the same parasite.

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Nova concepção para o método de Baermann-Moraes-Coutinho na pesquisa de larvas de nematódeos

Cited by 33 publications

References 0 publications

The Laboratory Diagnosis and Follow Up of Strongyloidiasis: A Systematic Review

The Laboratory Diagnosis and Follow Up of Strongyloidiasis: A Systematic Review

Changes in the reproductive biology of Biomphalaria glabrata experimentally infected with the nematode Angiostrongylus cantonensis

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

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