Morphological observations on the egg and first instar larva  of Metacutereba apicalis (Diptera: Cuterebidae)

Leite, Antônio Cesar Rios; Williams, Paul

doi:10.1590/s0074-02761989000100022

Cited by 7 publications

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“…The trichoid sensilla, with a single seta, observed in D.hominis, has also been observed in Ccuniculi, C,fontinefla and M.apicalis (Baker, 1986;Colwell, 1986;Leite & Williams, 1989). However, these three species lack basiconic sensilla, whereas the antennal sensory complex of D.hominis has three such sensilla.…”

Section: Discussionmentioning

confidence: 84%

“…In comparison with other Oestridae, the antennae of D.hominis are like those of the recently hatched larvae of Cuterebra cunicuii, C. fontinella and Metacuterebra apicalis, which were described, respectively, by Baker (1986). Colwell ( 1986) and Leite & Williams (1989). In contrast, Colwell & Scholl(l995) recorded that Gasterophilus intestinalis has cone-shaped antennae.…”

Section: Discussionmentioning

confidence: 98%

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Scanning electron microscopy studies on the first‐instar larva of Dermatobia hominis

Filippis

Leite

1997

Medical Vet Entomology

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First-instar larvae of Dermatobia hominis collected 1, 4 and 7 days after having penetrated experimentally infected rats, were studied by scanning electron microscope (SEM) observation. On the pseudocephalon there are basiconic and trichoid sensilla (antennal sensory complex), and basiconic, coeloconic and campaniform sensilla (maxillary sensory complex). The thoracic segments bear several rows of small, backwardly pointed, spines, and trichoid, campaniform, coeloconic and pit sensilla. The anterior spiracle is a minute opening. Both small and large spines directed posteriorly are on the first to fourth abdominal segments, which also bear coeloconic and companiform sensilla. These sensilla are present on the unarmed (fifth and sixth) and armed (seventh) abdominal segments. The seventh and the last (eight) abdominal segments have forwardly directed spines. Each spiracular plate has two spiracular openings and four spatulate-like structures called sun rays. The anus and the coeloconic sensilla are proeminent on the last segment. The results are compared with other parasitic dipteran larvae, and emphasize that the multiple types of sensilla on D.hominis larva may have importance in establishing the parasitic phase of the life cycle of this insect.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 98%

Scanning electron microscopy studies on the first‐instar larva of Dermatobia hominis

Filippis

Leite

1997

Medical Vet Entomology

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“…Three groups, each with six white rats, were infected by introducing two, five or ten first instar larvae of M. apicalis into the conjunctiva of the eye (Leite & Williams 1989). The infected rats were kept individually in wire covered plastic cages and were given a commercial rodent food and ad libitum access to water.…”

Section: Methodsmentioning

confidence: 99%

“…Based on LM and SEM studies, the egg and the first instar larva of M. apicalis have been described (Leite & Williams 1989). To complement our previous studies on the immature stages of M. apicalis, the second and third instar larvae, and the pupa are described herein. …”

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External Morphology of the Immature Stages of Metacuterebra apicalis

Leite

Williams

1997

Mem. Inst. Oswaldo Cruz

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The second and third instar larvae, and the pupa of Metacuterebra apicalis (Guérin-Menevilli), are described based on light and scanning electron microscope observations. Key words: Metacuterebra apicalis -Diptera -Cuterebridae -immature stage -larva -pupa -bot fly Cuterebrid bot flies are indigenous to the New World, where their larvae cause primary cutaneous myiasis in mammals, including man (Catts 1982). Metacuterebra is restricted to the Neotropical Region.In nature, the larval stages of M. apicalis parasitize rodents (Guimarães 1971, Guimarães et al. 1983, Bossi & Bergallo 1992, Vieira 1993. The life cycle of this species was described by Leite and Williams (1988).The morphological characters of the M. apicalis adults were partially described, under light microscope (LM) (Lutz 1917), and redescribed by LM and scanning electron microscope (SEM) observations (Leite & Williams 1989). Based on LM and SEM studies, the egg and the first instar larva of M. apicalis have been described (Leite & Williams 1989). To complement our previous studies on the immature stages of M. apicalis, the second and third instar larvae, and the pupa are described herein. MATERIALS AND METHODSThree groups, each with six white rats, were infected by introducing two, five or ten first instar larvae of M. apicalis into the conjunctiva of the eye (Leite & Williams 1989). The infected rats were kept individually in wire covered plastic cages and were given a commercial rodent food and ad libitum access to water. On the 9th and 18th days of infection, the rats were killed with ethyl ether and cutaneous lesions were removed. Second and third instar larvae were dissected from surrounding tissues. Some rodents were maintained until third instar larvae emerged from their hosts; these free-larvae were maintained until survivors transformed into pupae. Material destined for LM studies were fixed in Dietrich/San Jean fluid for 2 hr and then stored in 70% ethanol until examination. Material for SEM examination were killed in hot water (70°C), fixed in 70% ethanol, dehydrated in a ethanol series, submitted to CO 2 critical point drying and spattered with gold. At least eight specimens of each immature stage were studied. RESULTSSecond instar larva -On the ninth day of infection, the second instar larva (Fig. 1) is subcylindrical in shape, pale in colour, and measures 5.58 -7 mm in length and 1.24 -1.79 mm wide at the level of the thorax. The pseudocephalum bears tuberiform antennae. The first thoracic segment (Fig. 2) is surrounded anteriorly by small curved thorn-like spines, each with a backwardly directed simple tip. Occasionally, bare patches were seen in the anterior region. The second and the third thoracic segments (Fig. 2) are anteriorly encircled by backwardly directed thornlike spines with simple tips; a few spines with twoand three-pointed tips were seen on the third thoracic segment. From the first to the fifth abdominal segments (Fig. 3), anterior spines are similar to those on the third thoracic segment. The sixth abdominal segment bea...

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Bot Flies and Warble Flies (Order Diptera: Family Oestridae)

Colwell¹

2001

Parasitic Diseases of Wild Mammals

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Morphological observations on the egg and first instar larva of Metacutereba apicalis (Diptera: Cuterebidae)

Abstract: Descriptions are given of the egg and first intar larvar of Metacutereba apicalis (Diptera: Cuterebridae) when viewed by light and scanning electronic microscopes.

Cited by 7 publications

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Scanning electron microscopy studies on the first‐instar larva of Dermatobia hominis

Scanning electron microscopy studies on the first‐instar larva of Dermatobia hominis

External Morphology of the Immature Stages of Metacuterebra apicalis

Bot Flies and Warble Flies (Order Diptera: Family Oestridae)

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