Padrão isoenzimático da cepa Y do Trypanosoma cruzi após quimioterapia específica

Abstract

The isoenzyme pattern of the Trypanosoma cruzi Y strain recovered from mice inoculated with 15 x 10(4) blood trypomastigotes and previously treated with either Bay 2502 (Nifurtimox) or Ro 7-1051 (Benzonidazol) was analyzed in the following situations: a) strain resistant to Bay 2502 and again treated with the same drug; b) strain resistant to Bay 2502 and treated with Ro 7-1051; c) strain resistant to Ro 7-1051 and treated with that same drug. Although marked drug resistance was noted in all cases, the isoenzyme pattern of the Y strain for GPI, PGM, ALAT and ASAT remained throughout the same. The pattern was similar to that of the Peruvian strain, which also belongs to the same strain Type of the Y strain, but differed from those of the 21 SF (Type II) and Colombian (Type III) strains. Thus, the appearance of drug resistance in T. cruzi strain was not associated with a change in its isoenzymatic pattern.

Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory

“…The preservation of biological and biochemical characters of several strains after different laboratorial procedures as cryopreservation (Castro Silva et al 1988), passages into different species of triatomine (Magalhães et al l985, Magalhães & Andrade 1991), an the effect of chemotherapy (Castro Silva et al 1989, Marretto & Andrade 1994, has been observed. In the present study, the 21 SF strain, that has been maintained in laboratory by successive passages in mice for more than 15 years with stable biological and isoenzymic patterns has been used.…”