Metacyclogenesis of Trypanosoma cruzi in the vector Triatoma infestans

Schaub, Günter A.

doi:10.1590/s0074-02761988000500068

Cited by 11 publications

(2 citation statements)

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“…Mixed parasitic infections in a single host are common in nature in both mammalian and insect hosts, and can be considered a result of multiple sequential infections by feeding of the insect vector on different vertebrate hosts and/or parasite intake via coprophagy, which is a normal behaviour of triatomines [10-12]. Moreover, R. prolixus is regularly found in nature concomitantly infected with T. cruzi and Trypanosoma rangeli [13].…”

Section: Resultsmentioning

confidence: 99%

TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus

2014

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BackgroundRhodnius prolixus is an obligate haematophagous insect and one of the most important vectors of Trypanosoma cruzi, the causative agent of Chagas disease in the Americas. T. cruzi is a highly variable parasite which is not transmitted in the same efficiency by the different triatomine vectors. Because different T. cruzi genotypes are aetiopathologically divergent, further elucidation of the transmission abilities of different Chagas disease vectors is extremely important.FindingsIn the present study, the growth behaviour of two T. cruzi isolates, MDID/BR/1993/C45 (TcI) and TBRA/BR/1999/JCA3 (TcII), sharing the same microhabitat (intestinal tract) in single and mixed infections, was examined. The distribution patterns and parasite population densities were evaluated at 7, 14 and 21 days after feeding (daf) by quantification of parasites using Neubauer haemocytometric measurements and mini-exon PCR to identify TcI and TcII subpopulations. Parasitic colonization in the small intestine was more successful in the mixed infection model than the single infection models at 21 daf. In the rectal lumen and wall, the growth behaviour of the mixed infection was similar to that of the TcI group, although the total parasite number was lower. In the TcII group, no metacyclic trypomastigote forms were found. PCR analysis of the contents of each dissected region showed different genotype fractions in the mixed infection model, in which TcI seemed to be the predominant isolate.ConclusionThe different growth behaviour of the TcI and TcII isolates in single and mixed infection models demonstrated that possibly an intraspecific factor modulates parasitic development in the intestine of R. prolixus.

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Section: Resultsmentioning

confidence: 99%

TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus

2014

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“…The transformation of epimastigotes to metacyclic trypomastigotes (metacyclogenesis process), leading to forms that are eventually able to infect mammalian hosts, is of particular interest. In vivo and in vitro metacyclogenesis studies have demonstrated the presence of intermediate forms between epi-and trypomastigotes [3][4][5][6][7][8][9]. These intermediate forms were identified morphologically through the position of the kinetoplast relative to the nucleus, similar to the transitional stage described in the reduviid midgut [4,7,10,11].…”

Section: Introductionmentioning

confidence: 96%

Regulation of Phosphatidic Acid Levels in Trypanosoma cruzi

Gimenez

Santander

Villasuso

et al. 2011

Lipids

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Lipid kinases and phosphatases play essential roles in signal transduction processes involved in cytoskeletal rearrangement, membrane trafficking, and cellular differentiation. Phosphatidic acid (PtdOH) is an important mediator lipid in eukaryotic cells, but little is known regarding its regulation in the parasite Trypanosoma cruzi, an agent of Chagas disease. In order to clarify the relationship between PtdOH metabolism and developmental stages of T. cruzi, epimastigotes in culture were subjected to hyperosmotic stress (~1,000 mOsm/L), mimicking the environment in the rectum of vector triatomine bugs. These experimental conditions resulted in differentiation to an intermediate form between epimastigotes and trypomastigotes. Morphological changes of epimastigotes were correlated with an increase in PtdOH mass accomplished by increased enzyme activity of diacylglycerol kinase (DAGK, E.C. 2.7.1.107) and concomitant decreased activity of phosphatidate phosphatases type 1 and type 2 (PAP1, PAP2, E.C. 3.1.3.4). Our results indicate progressive increases of PtdOH levels during the differentiation process, and suggest that the regulation of PtdOH metabolism is an important mechanism in the transition from T. cruzi epimastigote to intermediate form.

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Rapid isolation of metacyclic trypomastigotes of Trypanosoma cruzi from feces and urine of the vector

Schaub

1991

Acta Tropica

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Metacyclogenesis of Trypanosoma cruzi in the vector Triatoma infestans

Cited by 11 publications

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TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus

TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus

Regulation of Phosphatidic Acid Levels in Trypanosoma cruzi

Rapid isolation of metacyclic trypomastigotes of Trypanosoma cruzi from feces and urine of the vector

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