The role of hematopoietic growth factors in the fate of an infection with Leishmania mexicana amazonensis: an attempt at a unifying hypothesis

Barcinski, M A; Charlab, Rosane; Soares, Luis; Moreira, M E C; Zalis, Mariano; Magalhães, Ana

doi:10.1590/s0074-02761988000500035

Cited by 3 publications

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“…Alternatively, the PCD pathway could be a response to signals from the host such as growth factors or cytokines. 56 In addition, PCD may be beneficial to the organism to maintain clonal characteristics by self-destruction of some cells and ensuring the propagation of other cells for the overall benefit of the parasite survival. 18,57 For example, in the case of Leishmania promastigotes, which spend a significant time in the insect vector and differentiate into metacyclic forms before being transmitted to the mammalian host.…”

Section: Discussionmentioning

confidence: 99%

Programmed cell death in the unicellular protozoan parasite Leishmania

Lee¹,

Bertholet²,

Debrabant³

et al. 2002

Cell Death Differ

254

191

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In the present study we have demonstrated some features characterizing programmed cell death (PCD) in the unicellular protozoan parasite Leishmania donovani, the causative agent of visceral Leishmaniasis. We report that PCD is initiated in stationaryphaseculturesofpromastigotesandbothinactively growing cultures of axenic amastigotes and promastigotes upon treatment with anti Leishmanial drugs (Pentostam and amphotericin B). However, the two cell types respond to antileishmanial drugs differently. The features of PCD in L. donovani promastigotes are nuclear condensation, nicked DNA in the nucleus, DNA ladder formation, increase in plasma membrane permeability, decrease in the mitochondrial membrane potential (DC m ) and induction of a PhiPhiLux (PPL)-cleavage activity. PCD in both stationary phase culture and upon induction by amphotericin B resulted first in the decrease of mitochondrial membrane potential followed by simultaneous change in plasma membrane permeability and induction of PPL-cleavage activity. Of the total PPL-cleavage activity, several caspase inhibitors inhibited a significant amount (21 ± 34%). Inhibitors of cathepsin or calpain did not inhibit PPL-cleavage activity. Taken together this study demonstrates that the characteristic features of PCD exist in unicellularprotozoanLeishmaniadonovani.Theimplicationof PCD on the Leishmania pathogenesis is discussed. Cell Death and Differentiation (2002) 9, 53 ± 64.

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Section: Discussionmentioning

confidence: 99%

Programmed cell death in the unicellular protozoan parasite Leishmania

Lee¹,

Bertholet²,

Debrabant³

et al. 2002

Cell Death Differ

254

191

View full text Add to dashboard Cite

show abstract

Programmed cell death in trypanosomatids and other unicellular organisms

Debrabant

Lee

Bertholet

et al. 2003

International Journal for Parasitology

157

114

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Granulocyte‐Macrophage Colony‐Stimulating Factor is a Growth‐Factor for Promastigotes of Leishmania mexicana amazonensis

Charlab

Blaineau

Schechtman

et al. 1990

The Journal of Protozoology

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In this paper we show that murine lung conditioned medium (LCM) displays, in addition to its already described colony-stimulating activity on bone marrow cells, a potent growth-stimulating activity on promastigotes of Leishmania mexicana amazonesis. Immunoprecipitation of LCM with an antibody specific for murine granulocyte-macrophage colony stimulating factor (GM-CSF) abrogates both activities, indicating that the leishmanial growth-promoting activity is due to the presence of GM-CSF on LCM. Furthermore, recombinant GM-CSF (rGM-CSF) added to the culture medium or to the immunoprecipitated LCM is able to respectively induce or to partially recover the growth-promoting activity of the LCM. Sequential in vitro passages of the parasite induces a progressive loss of sensitivity to the growth-factor. Parasite forms recently collected from lesions are significantly more responsive to the growth-factor than forms already adapted to grow in culture. Since it has been shown that several different microorganisms display receptors for vertebrate-like hormones and that GM-CSF is able to enhance a cutaneous leishmanial lesion, our results permit us to raise the hypothesis that a direct interaction between a host-derived hormone and a pathogenic microorganism can be of importance in defining the fate of an infection. The fact that GM-CSF is produced by cells that actively participate in a leishmanial infection (T-lymphocytes and macrophages) reinforces our hypothesis.

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The role of hematopoietic growth factors in the fate of an infection with Leishmania mexicana amazonensis: an attempt at a unifying hypothesis

Cited by 3 publications

References 0 publications

Programmed cell death in the unicellular protozoan parasite Leishmania

Programmed cell death in the unicellular protozoan parasite Leishmania

Programmed cell death in trypanosomatids and other unicellular organisms

Granulocyte‐Macrophage Colony‐Stimulating Factor is a Growth‐Factor for Promastigotes of Leishmania mexicana amazonensis

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