Trypanosoma Cruzi: major clones rather than principal zymodemes

Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory

“…However it has been admitted that a limited number of "principal" clones could be circulating into different geographical areas (Tibayrenc & Brenière 1988). Considering this hypothesis, the homogeneity here disclosed for the clones and subclones of the 21SF strain, could represent the presence of a "principal" clone, corresponding to Type II, zymodeme 2 strains.…”

Isoenzyme profiles of Trypanosoma cruzi stocks from different areas of Paraguay

“…These clones are genetically well separated and they belong to T. cruzi I and T. cruzi II groups, respectively (Anon 1999). They correspond to major clones frequently isolated in South America and they are the main clones found in the domestic Bolivian cycle (Tibayrenc & Brenière 1988, Brenière et al 1998 Isolation and maintenance of the stocks -The stocks were obtained by hemoculture, xenoculture and mice inoculation, maintained and amplified at 28ºC in LIT medium (liver infusion tryptose) (Camargo 1964) with 50 µg/l of hemin, supplemented with 20% fetal bovine serum, 100 UI of penicillin and 100 µg of streptomycin per ml of medium.…”

Taxonomy of Trypanosoma cruzi: a commentary on characterization and nomenclature

“…As the techniques were applied on different collections of strains not all strains or isolates within each subdivision may exactly correspond in all of the studies. a: also referred to as major clone (Tibayrenc & Breniere 1988) or clonet (Tibayrenc & Ayala 1991); b: also referred to as Discrete Typing Unit (DTU) (Tibayrenc 1998b). netic integrity over long time intervals and over great distances, that is, their genomes are not rapidly broken down or reshuffled by recurrent mutation and recombination is known as the clone concept (Orskov & Orskov 1983). Tibayrenc et al (1986) have proposed this model as the main population genetic structure for T. cruzi.…”