Insect immunity: a gene system for antibacterial proteins

Boman, Hans G.; Faye, Ingrid; Gan, Runliang; Guðmundsson, Guðmundur H.; Lidholm, Dan-Anders; Lee, J Y; Xanthopoulos, Kleanthis G.

doi:10.1590/s0074-02761987000700022

Cited by 10 publications

(5 citation statements)

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“…Certain groups of these factors have significant impacts on the ability of insect vectors to become infected with and subsequently transmit human disease pathogens (5,28,53). One group of insect immune peptides is the cecropins, which derive their name from the saturniid Hyalophora cecropia, from which they were originally described (14,15,79). Cecropin A, a member of this class of naturally occurring peptides, has been studied extensively, and the gene that encodes it has been cloned, sequenced, and expressed (39,49).…”

Section: Expression Of the Antitrypanosomal Peptide Cecropin A In Rmentioning

confidence: 99%

Bacterial Symbionts of the Triatominae and Their Potential Use in Control of Chagas Disease Transmission

Beard

Cordón-Rosales

Durvasula

2002

Annu. Rev. Entomol.

214

158

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Chagas disease is caused by the parasitic protozoan Trypanosoma cruzi and transmitted by insects in the family Reduviidae, subfamily Triatominae, commonly known as kissing bugs. Because these insects feed throughout their entire developmental cycle on vertebrate blood, they harbor populations of symbiotic bacteria in their intestinal track that produce nutrients that are lacking in the insects' limited diet. It is possible to cultivate these bacteria, genetically modify them, and place them back into their insect host, thus generating a paratransgenic insect. This procedure has allowed the expression of antitrypanosomal gene products in the insect gut, thereby resulting in insects that are incapable of transmitting Chagas disease. A method has been developed that would allow introduction and spread of genetically modified symbionts into natural populations of kissing bugs, thus leading potentially to a transgenic intervention tool for use as a part of an integrated vector control approach.

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Section: Expression Of the Antitrypanosomal Peptide Cecropin A In Rmentioning

confidence: 99%

Bacterial Symbionts of the Triatominae and Their Potential Use in Control of Chagas Disease Transmission

Beard

Cordón-Rosales

Durvasula

2002

Annu. Rev. Entomol.

214

158

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“…Antimicrobial peptides are an evolutionarily conserved component of the innate immune response in insects, and are synthesized primarily in fat body and hemocytes, and subsequently get secreted into the hemolymph upon microbial infection (Yamakawa & Tanaka 1999). These molecules have been known to fall into four groups: cecropins (Steiner et al 1981), attacins (Hultmark et al 1983), lysozyme (Boman et al 1987), and defensins (Lambert et al 1989). In this study, the lysozyme 3-like (XP_001600829.2) gene was identified as immune response factor.…”

Section: Discussionmentioning

confidence: 92%

Expressed Sequence Tags (ESTs) analysis of Tenebrio molitor larvae

Jeong

Kang

Hwang

et al. 2013

Entomological Research

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Tenebrio molitor has been seriously investigated as a model insect in elucidating Toll signaling pathway and related regulators of innate immunity. However, little is known with regards to the genomic information in T. molitor. In an attempt towards exploiting the rich transcriptomics data that would offer further insights into the study on insect immunity through potential screening of immune‐related genes in the model insect, we constructed a cDNA library (library titer = 5.0 × 105pfu/ml) of T. molitor larvae and analyzed expressed sequence tag (EST) sequences from 1056 clones. The base calling and quality check of obtained chromatogram files were performed by using the Phred program (trim_alt 0.05 (P‐score > 20). After removal of vector and 100 bp and less sequences, 1023 sequences were generated having an average insert size of 792 bp, including 160 clusters, 164 contigs and 387 singletons through clustering and assembling process using the TGI Clustering Tools (TGICL) package. The unique EST sequences were searched against the NCBI nr database by local BLAST (blastx, E < e−5) with 940 sequences showing significant hits. Subsequently, KOG (clusters of orthologous groups for eukaryotic complete genomes) analysis was conducted (blastx, E < e−10) towards prediction of transcriptomal functions, leading to the categorization of 638 genes. The majority of genes belonged to Z category (cytoskeleton‐related genes), R category (general function prediction), and C category (energy production and conversion related genes). These basic EST datasets and their bioinformatics analysis will be helpful in investigating the biological mechanism and molecular pathway related genes involved in innate immunity mechanisms of T. molitor.

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“…For similar reasons we are interested in the genes that code for insect immune proteins, such as the attacins, cecropins and diptericin (Boman et al, 1987).…”

Section: \G2mentioning

confidence: 99%

The control of insect-borne disease through recombinant DNA technology

Eggleston

1991

Heredity

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Insect immunity: a gene system for antibacterial proteins

Cited by 10 publications

References 0 publications

Bacterial Symbionts of the Triatominae and Their Potential Use in Control of Chagas Disease Transmission

Bacterial Symbionts of the Triatominae and Their Potential Use in Control of Chagas Disease Transmission

Expressed Sequence Tags (ESTs) analysis of Tenebrio molitor larvae

The control of insect-borne disease through recombinant DNA technology

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