B cell dependent T lymphocyte responses in leishmaniasis

Sacks, David L.

doi:10.1590/s0074-02761987000500015

Cited by 2 publications

(2 citation statements)

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“…The latter has been associated with CD4+ memory T-cell populations (2,11,25), and it could explain the high proliferative background levels observed within the infected animals and further substantiates the similarity of the vervet model with the human infection, in which recovery is associated with lasting immunity. Secondly, our observations of increased numbers of T cells but not B cells are in keeping with the concept of T-cell activation in the cure response and B-cell expansion in disease progression (42,43). The lack of B-cell expansion may also correlate with the inability to stimulate antigen-specific IL-5 production and would be further explained if the high levels of IL-2/IL-4 observed with the T-cell expansion were due to IL-2.…”

Section: Discussionsupporting

confidence: 85%

“…4. Because progressive Leishmania infections have been associated with polyclonal B-cell activation and increased antibody production (42,43), it was interesting to observe that cured animals showed no increase in the percentage of B lymphocytes. Recovery from infection was, however, associated with a dramatic increase in the percentage of circulating T cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Cell-mediated responses of immunized vervet monkeys to defined Leishmania T-cell epitopes

et al. 1994

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A population of vervet monkeys was immunized with killed parasites and infected with Leishmania major promastigotes either by needle or by infected-fly bite. The responses of recovered monkeys to mitogens, killed parasites, and molecularly defined T-cell epitopes were then compared with those of control animals. Peripheral blood mononuclear cells (PBMC) from both naive and recovered animals proliferated strongly in response to both B-and T-cell mitogens, although the responses of the recovered animals were less strong than those of the naive animals. Cells from recovered vervets, but not those from naive vervets, also proliferated in response to parasite antigens and synthetic T-cell epitopes. Likewise, cells from recovered animals released gamma interferon and either interleukin 2 (IL-2) or IL-4 into culture media in response to both of the above-mentioned antigens, whereas cells from control animals did not. The fact that no IL-5 could be measured following parasite antigen or synthetic T-cell epitope stimulation of PBMC suggested that cells proliferating in response to these molecules belonged to the Thl subset. Phenotypic analysis of the PBMC showed a marked increase in T-cell but not B-cell populations in recovered animals. Among this population was an increased number of CD45RO0 memory cells. The data from this study are in keeping with the earlier finding that vervet monkeys provide an excellent model system for leishmaniasis. Further, these data support the contention that synthetic T-cell epitopes are prime candidates for molecularly defined Leishmania vaccines.

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Section: Discussionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%