Endocytosis of the humam immunodeficiency virus in vitro

Bauer, Pierre Georges; Barth, Ortrud Monika; Pereira, Margheritte S.

doi:10.1590/s0074-02761987000300023

Cited by 6 publications

(3 citation statements)

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“…HIV fuses at the cell surface and entry is not affected by weak bases and carboxylic ionophores (McClure et al, 1987;Stein et al, 1987). Virus particles are also observed in endocytic coated pits and coated vesicles (Bauer et al, 1987;D. Pauze, personal communication).…”

Section: Retrovirusesmentioning

confidence: 99%

Virus Entry into Animal Cells

Marsh

Helenius

1989

Advances in Virus Research

568

414

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Section: Retrovirusesmentioning

confidence: 99%

Virus Entry into Animal Cells

Marsh

Helenius

1989

Advances in Virus Research

568

414

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“…Furthermore, CD4 and p56 lck can be dissociated by agents such as phorbol esters (15,34) or HIV gp120 (4,16), leading to enhanced CD4 endocytosis. Finally, although fusion figures for HIV have been observed at the cell surface by electron microscopy (13,35,45), HIV virions are also frequently associated with endocytic clathrin-coated vesicles, and internalized virions can be observed in the process of fusion (1,13,30,35,37). Since only a small proportion of the virions in HIV preparations are actually infectious (22), it is unclear whether the viruses observed in these images would have gone on to establish productive infections.…”

mentioning

confidence: 99%

Role of CD4 endocytosis in human immunodeficiency virus infection

Pelchen–Matthews

Clapham

Marsh

1995

J Virol

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We have analyzed the role of CD4 endocytosis in human immunodeficiency virus (HIV) entry by measuring the infection of HeLa cells expressing various CD4 constructs with endocytosis rates of between 0.2 and 30%/min in a quantitative infectious focus assay. For a number of laboratory-adapted HIV-1 and HIV-2 strains, the highest levels of infection were found on cells with very limited CD4 endocytosis, while cells with efficient CD4 uptake were only poorly infectable, suggesting that CD4 internalization is not required for HIV entry. This was confirmed in a modified assay involving prebinding of HIV-1 LAI to HeLa-CD4 cells at 4؇C, synchronized virus entry during warming to 37؇C, and neutralization of virions remaining at the cell surface with anti-V3 loop antibodies. Warming cells in hypertonic medium inhibited CD4 endocytosis but did not affect the rate or the extent of infection. These studies confirm that HIV infection does not require endocytosis and that laboratory-adapted virus strains can enter HeLa-CD4 cells by fusion at the plasma membrane.

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“…However, the same viruses also have been observed inside endocytic vesicles, suggesting that the fusion event might also occur from within endocytic vesicles (Campadelli-Fiume et al, 1988;Bauer et al, 1987). If so, resistance to inhibition by lysosomotropic agents is not an absolute indication that fusion occurred at the cell surface.…”

Section: Entry Of Ph-independent Group Of Virusesmentioning

confidence: 98%

Porcine reproductive and respiratory syndrome virus infection of cells: characterization of the early stages of virus infection and the major structural proteins of the virus

Kreutz

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Endocytosis of the humam immunodeficiency virus in vitro

Cited by 6 publications

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Virus Entry into Animal Cells

Virus Entry into Animal Cells

Role of CD4 endocytosis in human immunodeficiency virus infection

Porcine reproductive and respiratory syndrome virus infection of cells: characterization of the early stages of virus infection and the major structural proteins of the virus

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