Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

Abstract

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from an infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

Field evaluation of four novel enzyme immunoassays for Chagas’ disease in Venezuela blood banks: comparison of assays using fixed‐epimastigotes, fixed‐trypomastigotes or trypomastigote excreted–secreted antigens from two Trypanosoma cruzi strains

“…In fact, an extraordinarily wide range of EIAs based on different T. cruzi antigens has been described. These include crude sonicated epimastigotes (Breniere et al , 1985; Almeida et al , 1997; Monteon et al , 1997; Pinho et al , 1999), proteins extracted from epimastigotes (Schechter, 1987; Cuna et al , 1989; Solana et al , 1995; Partel & Rossi, 1998), fixed‐whole epimastigotes (De Hubsch et al , 1988, 1989; Carbonetto et al , 1989; Antas et al , 2000), fixed and sonicated amastigotes (Araujo & Guptill, 1984), sonicated and purified trypomastigote glycoconjugate antigens (Almeida et al , 1997; Aznar et al , 1997), recombinant proteins (Cetron et al , 1992; Godsel et al , 1995; Almeida et al , 1997; da Silveira et al , 2001; Ferreira et al , 2001; Matsumoto et al , 2002) and trypomastigote excretory–secretory antigens (TESA) (Umezawa et al , 1996, 2001; Kesper et al , 2000; Nakazawa et al , 2001; Matsumoto et al , 2002). Epimastigote antigens have been used in most of these studies (84%) due to ease of culture and good antigen yield (Monteon et al , 1997).…”