Localization  of protective malarial antigens by immuno-electron microscopy

Aikawa, Masamichi

doi:10.1590/s0074-02761986000600026

Cited by 5 publications

(1 citation statement)

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“…The antibody selected from the immune rhesus serum by this method immunoprecipitated several Triton X-100-soluble molecules from [35S]methionine metabolically labeled schizonts; therefore, the protein of the dense granules recognized by this serum cannot be defined at this time. For immunoelectron microscopy, extracellular merozoites and infected erythrocytes were fixed with 1% paraformaldehyde-0.1% glutaraldehyde in 0.1 M PBS at pH 7.3 and embedded in LR white resin (Polysciences, Inc., Warrington, Pa.) (1). Sections were etched with a saturated aqueous solution of sodium metaperiodate and incubated for 30 min in 0.1 M PBS containing 5% nonfat dry milk and 0.01% Tween 20 (PBS-milk-Tween).…”

Section: Figmentioning

confidence: 99%

Release of merozoite dense granules during erythrocyte invasion by Plasmodium knowlesi

et al. 1989

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We used immunoelectron microscopy to study the fate of dense granules during the invasion of erythrocytes by Plasmodium knowlesi merozoites. When merozoites entered host cells, dense granules moved to the pellicle, released their contents into the parasitophorous vacuole space, and then moved into fingerlike channels of the vacuole membrane. This is the first report showing that the content of dense granules of P. knowlesi is different from the contents of rhoptries and micronemes and is associated with the formation of channels from the parasitophorous vacuole.

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Section: Figmentioning

confidence: 99%