A comparison of simian rotavirus SA11 preparations maintained in different laboratories

Pereira, H. G.; Gouvêa, Vera; Fialho, Alexandre Madi

doi:10.1590/s0074-02761986000400005

Cited by 13 publications

(8 citation statements)

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“…Fecal samples were also tested for Salmonella species using a standard technique, while Coccidium species and Cryptosporidium species were detected by a sucrose floatation method. In addition, fecal samples were examined for rotavirus double-stranded RNA by polyacrylamide gel electrophoresis [17].…”

Section: Clinical Specimensmentioning

confidence: 99%

Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows

et al. 2011

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Bovine torovirus (BToV) is recognized as an enteric pathogen of calves, but its etiological role in diarrhea and epidemiological characterization in adult cows remain unclear. In 2007-2008, three outbreaks of epidemic diarrhea occurred in adult cows at three dairy farms in Niigata Prefecture, Japan. BToV was the only enteric pathogen detected in these outbreaks, as determined by electron microscopy, reverse transcription-PCR, bacteria and parasite tests of fecal samples, and antibody tests with paired sera. The epidemiological features of the three outbreaks were similar to those of bovine coronavirus infection, except for the absence of bloody diarrhea, with diarrhea spreading among most adult cows, but not in calves, within several days and diarrhea lasting for 3-5 days with anorexia. Decreased milk production and mild respiratory symptoms were also observed in two of the outbreaks. Nucleotide sequence analysis of the BToV nucleocapsid, spike, and hemagglutinin-esterase (HE) genes revealed a close relatedness among the detected BToV strains from each outbreak and those of Japanese BToV strain Aichi/2004. Furthermore, we isolated a BToV strain, designated Niigata (TC), from a fecal sample using a human rectal tumor cell line. Sequence analysis of this isolate and Aichi/2004 indicated that both strains have truncated HE genes with deletions in the 3' region that occurred through cell culture-adaptation. The short projections that are believed to be formed by the HE protein on virus particles were not observed in these cultured strains by electron microscopy. Taken together, these results suggest that BToV causes epidemic diarrhea in adult cows and should be included in the differential diagnosis of diarrhea in adult cows. In addition, our findings indicate that the HE protein of BToV may not be necessary for viral replication.

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Section: Clinical Specimensmentioning

confidence: 99%

Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows

et al. 2011

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“…The discovery of "O"-agent genes in the SA11-Both and the SA11-4F-like viruses suggests that the size heterogeneity noted for other SA11 genes may likewise be due to reassortment events involving the "O" agent (Pereira et al, 1984;1986). Whether this is the case awaits sequencing of SA11 strains not in our possession.…”

Section: Sa11 Reassortment With the O Agentmentioning

confidence: 99%

“…Early success in the high-titer amplification of SA11 in cell culture systems supplemented with trypsin has led to the distribution of this virus throughout the world and its widespread application as the prototypic strain for studies on rotavirus growth, virulence, pathogenesis, virion architecture, genome replication, and viral protein structure and function (Estes, 2001). Electrophoretic comparison of the genome segments of the various SA11 strains used by different laboratories has shown that heterogeneity exists in several genome segments, including those encoding VP4 (gene 4), NSP1 (gene 5), and NSP2 (gene 8) or NSP3 (gene 7) (Pereira et al, 1984(Pereira et al, , 1986. Sequencing has indicated that the fast (F) migrating gene 4 RNA detected in some SA11 strains is similar to the gene 4 RNA of bovine strains NCDV (P6[1]:G6) (Mattion and Estes, 1991) and C486 (P6[1]:G6) (Mitchell and Both, 1989).…”

Section: Introductionmentioning

confidence: 99%

Genome heterogeneity of SA11 rotavirus due to reassortment with “O” agent

et al. 2007

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Derivatives of the rotavirus SA11-H96 strain, isolated in 1958 from an overtly healthy vervet monkey, have been used extensively to probe the viral life cycle. To gain insight into the phenotypic and genotypic differences among SA11 isolates, we sequenced the segmented double-stranded RNA genomes of SA11-H96 (P5B[2]:G3), two SA11-4F-like viruses (P6[1]:G3), two SA11-4F-like viruses with gene 5 rearrangements, and relevant segments of SA11 temperature-sensitive mutants and the "O" (Offal) agent (P6[1]:G8), a rotavirus isolated in 1965 from abattoir waste. This analysis indicates that the only complete genomic sequence previously reported for SA11 (Both) is instead that of a reassortant, originating like the SA11-4F-like viruses, from the introduction of an "O" agent gene into the SA11 genetic background. These results, combined with identification of mutations that correlate with altered growth properties and ts phenotype, emphasize the importance of considering segment origin and sequence variation in interpreting experimental outcomes with SA11 strains.

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“…Fragments were visualized after silver staining according to data published elsewhere [Pereira et al, 1986].…”

Section: Pagementioning

confidence: 99%

Detection of group a human rotavirus G9 genotype circulating in Córdoba, Argentina, as early as 1980

et al. 2006

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The incidence of human rotavirus G types was determined over a 25-year period (1979-2003) by using reverse transcription-PCR (RT-PCR) to examine 519 stool specimens found to be positive for rotavirus by enzyme linked immunosorbent assay (ELISA) or polyacrylamide gel electrophoresis (PAGE). These stool samples were obtained from children under 3 years old who had been treated for acute diarrhea at public hospitals in Córdoba, Argentina. The present study describes the continued circulation of the common human G types G1 (53.8%), G2 (10.2%), G3 (4.4%), and G4 (27%), and also the detection of the unusual types G8 (0.5%) and G9 (4.2%). Genotype G9 was detected during the 1980-1988 and 1997-2003 periods at relatively low rates. Rotavirus G types distribution was independent of age (1-18 months), gender or out-patient or in-patient status. Unexpectedly, 44.6% of mixed infections were detected, involving common and unusual genotypes. Overall, 95.4% of the typed strains belonged to the most prevalent human serotypes (G1-G4) but the detection of G9 infection throughout this study period highlights the importance of this serotype as a human pathogen.

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A comparison of simian rotavirus SA11 preparations maintained in different laboratories

Cited by 13 publications

References 0 publications

Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows

Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows

Genome heterogeneity of SA11 rotavirus due to reassortment with “O” agent

Detection of group a human rotavirus G9 genotype circulating in Córdoba, Argentina, as early as 1980

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