Comportamento das cepas Y e Peruana do Trypanosoma cruzi no camundongo, após passagem em diferentes meios

Abstract

The behavior of two strains of Trypanosoma cruzi (Y and Peruvian strains) in experimental mouse infection, after being passed through different conditions of maintainance and cultivation was studied. The conditions were: Warren's acellular culture medium, cryopreservation in liquid Nitrogen, passage through the insect vector and direct blood passage from mice to mice. The parameters considered for comparative study were as follows: parasitemia, mortality rate, maximum survival time, morphology of parasites in peripheral blood, tissue tropism and histopathological lesions. Each experimental group consisted of two sub-groups according to the inocula: 10.000 or 50.000 trypomastigotes. The basic characteristics of the strains remained unchanged. These were macrophagotropism, predominance of slender forms of the parasite in early infection and 100 per cent mortality rate in the acute phase of the infection. However, decrease in the virulence was observed when the culture forms were used or when the infection with low inoculum was used (10.00 forms). Therefore the main biological characteristics of the strains tended to remain the same, regardless of the conditions used for maintainance and cultivation.

Morphological, biological and molecular characterization of three strains ofTrypanosoma cruziChagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated fromTriatoma sordida(Stal) 1859 (Hemiptera, Reduviidae) and a domestic cat

“…That variation is frequently seen when a new strain of the parasite is isolated, and it is directly related to the stabilization of the relationship between the parasite and the vertebrate host (Albuquerque, 2001). Inoculation of laboratory animals with T. cruzi strains has resulted in a variable mortality rate (Andrade, 1974;Magalhães et al 1985;Barata et al 1988). In this study, such rate was equal to zero for all strains.…”

“…Inoculation of laboratory animals with T. cruzi strains has resulted in a variable mortality rate (Andrade, 1974 ; Magalhães et al . 1985 ; Barata et al . 1988 ).…”

Comparative Analysis by Polymerase Chain Reaction Amplified Minicircles of Kinetoplast DNA of a Stable Strain of Trypanosoma cruzi from São Felipe, Bahia, its Clones and Subclones: Possibility of Predominance of a Principal Clone in this Area

“…This view is important when we consider T. cruzi strains and the correlations of the genetical patterns with biological behavior. The "stability" of T. cruzi strains can be considered as the possibility of maintenance of its populations in an equilibrium after several laboratory passages in different conditions or through different procedures such as axenic cultures, animal passages, cryopreservation or vector passages (Magalhães et al 1985, Magalhães & Andrade 1991. The clonal homogeneity could be considered as an important factor to this stability.…”

Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples

“… References: a [ 6 ]; b [ 26 ]; c [ 27 ]; d [ 28 ]; e [ 29 ]; f [ 30 ]; g [ 31 ]; h [ 32 ]; i [ 33 ] j [ 34 ]; k [ 22 ]; l [ 23 ]; m [ 35 ]; n [ 36 ]; o [ 37 ]; p [ 38 ]; q [ 39 ]; r [ 40 ]; s [ 41 ]. DTU, Discrete Typing Unit; nd, no data.…”