Studies on the relationship between lectin binding carbohydrates and different strains of Leishmania from the New World

Schottelius, Justus Georg; Costa, Salvatore

doi:10.1590/s0074-02761982000100003

Cited by 18 publications

(10 citation statements)

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“…1 In these cells, they transform into amastigotes within parasitophorus vacuoles, causing broad spectrum diseases in humans, ranging from cutaneous to mucosal and diffuse leishmaniasis. 2,3 Leishmania (L.) amazonensis has also been implicated in non-cutaneous forms of the disease such as mucosal, visceral, and post-kala-azar dermal leishmaniasis. 4,5 Visceral leishmaniasis can also be caused by L. (L.) amazonensis in patients with associated infections with human immunodeficiency virus.…”

Section: Introductionmentioning

confidence: 99%

Central Nervous System Involvement in Experimental Infection With Leishmania (Leishmania) Amazonensis

Abreu-Silva

Calabrese²,

Tedesco³

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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Abstract. We describe the pathologic alterations of the central nervous system (CNS) observed in experimental tegumentary leishmaniasis in BALB/c and Swiss mice. The mice were subcutaneously infected with 10 4 amastigotes of Leishmania (Leishmania) amazonensis. Animals were killed and brains were removed for histologic and immunocytochemical studies. Histologic examination showed that 66.6% of infected mice had a discrete hyperemia and inflammatory infiltrate in the meninges, composed of mononuclear cells and neutrophils with no detectable parasites. However, parasitized macrophages were detected in the cerebral parenchyma, as well as mast cells, lymphocytes, and polymorphonuclear cells. Necrosis in the cerebral parenchyma was also observed. Confocal fluorescence microscopy showed that CD8 + T lymphocytes are the major component of the inflammatory infiltrate in the CNS. In addition to these cells, CD4 + , CD11b, and dendritic cells are present, in small numbers, in the inflammatory processes of the CNS. Thus, L. amazonensis is able to cross the blood-brain barrier and cause significant pathologic changes in the CNS.

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Section: Introductionmentioning

confidence: 99%

Central Nervous System Involvement in Experimental Infection With Leishmania (Leishmania) Amazonensis

Abreu-Silva

Calabrese²,

Tedesco³

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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“…(L . ) amazonensis (MHOM/BR/1976/MA-76) obtained from a human case of diffuse infection and characterized by isoenzyme [9] and lectin techniques [10] was maintained in the laboratory by successive passages in BALB/c mice. Prior to infection, parasites were isolated from a non-ulcerated nodular lesion in the footpad and amastigote viability was checked with erythrosine B by light microscopy.…”

Section: Methodsmentioning

confidence: 99%

Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis

Almeida-Souza¹,

Cardoso²,

Souza³

et al. 2016

PLoS Negl Trop Dis

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The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography–mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg.kg-1.day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment.

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“…The L. amazonensis, MHOM/BR/76/Ma-5 strain, isolated from a man with diffuse cutaneous leishmaniasis (DCL), was characterized by isoenzymes and lectins (Grimaldi et al, 1991, Schottelius andGonçalves da Costa, 1982). Promastigotes were maintained by serial passages in our laboratory, in LIBHIT medium (Gonçalves da Costa and Lagrange, 1981).…”

Section: Parasitesmentioning

confidence: 99%

Ocular experimental leishmaniasis in C57BL/10 and BALB/c mice induced by Leishmania amazonensis infection

Calabrese

Silva

Hardoim

et al. 2013

Experimental Parasitology

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There are few studies on human ocular leishmaniasis found in the literature. The purpose of this study was to describe experimental ocular leishmaniasis, caused by Leishmania amazonensis evaluating two different infection routes: intravitreal and instillation in C57BL/10 and BALB/c mice. In this work all animals presented low anti-Leishmania IgM and IgG titers regardless of the infection route or mouse strain. The histopathological eye analysis showed that the mice inoculated by the intravitreal route developed more severe lesions, presenting parasites in the anterior region of the eye 60 days after infection. The C57BL/10 mice presented cells containing parasitophorous vacuoles associated with pigmented cells and inflammatory infiltrate, which included mast cells. Ninety days after infection no parasites could be found in either mouse strain, which led us to hypothesize that parasites had been eliminated. In this context, we show that both intravitreal and instillation routes were effective in promoting ocular leishmaniasis infections in C57BL/10 and BALB/c mice. There were no differences in the parasite infection between the two mouse models and it mimicked the ocular lesions described in symptomatic dogs in endemic areas of visceral leishmaniasis.

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Studies on the relationship between lectin binding carbohydrates and different strains of Leishmania from the New World

Cited by 18 publications

References 0 publications

Central Nervous System Involvement in Experimental Infection With Leishmania (Leishmania) Amazonensis

Central Nervous System Involvement in Experimental Infection With Leishmania (Leishmania) Amazonensis

Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis

Ocular experimental leishmaniasis in C57BL/10 and BALB/c mice induced by Leishmania amazonensis infection

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