Trypanosoma cruzi: circulating antigens

Bongertz, Vera; Hungerer, Klaus-Dieter; Galvão–Castro, Bernardo

doi:10.1590/s0074-02761981000100008

Cited by 8 publications

(4 citation statements)

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“…It is possible that EVs released from infected host cells may in part account for T. cruzi antigens previously detected in the circulatory system or urine of infected patients (72)(73)(74)(75), making EVs an attractive and tractable biological tool for facilitating direct antigen detection in fluids of Chagas disease patients. Indeed, EVs are emerging as a unique mechanism for enriching low-level antigens for cancer diagnosis (76), and recent studies using T. cruzi-specific RNA aptamers detected antigens in TESA preparations or serum from mice with acute or chronic T. cruzi infection (77).…”

Section: Discussionmentioning

confidence: 99%

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

et al. 2017

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Chagas disease, caused by Trypanosoma cruzi, although endemic in many parts of Central and South America, is emerging as a global health threat through the potential contamination of blood supplies. Consequently, in the absence of a gold standard assay for the diagnosis of Chagas disease, additional antigens or strategies are needed. A proteomic analysis of the trypomastigote excreted-secreted antigens (TESA) associated with exosomal vesicles shed by T. cruzi identified ϳ80 parasite proteins, with the majority being trans-sialidases. Mass spectrometry analysis of immunoprecipitation products performed using Chagas immune sera showed a marked enrichment in a subset of TESA proteins. Of particular relevance for diagnostic applications were the retrotransposon hot spot (RHS) proteins, which are absent in Leishmania spp., parasites that often confound diagnosis of Chagas disease. Interestingly, serological screens using recombinant RHS showed a robust immunoreactivity with sera from patients with clinical stages of Chagas ranging from asymptomatic to advance cardiomyopathy and this immunoreactivity was comparable to that of crude TESA. More importantly, no cross-reactivity with RHS was detected with sera from patients with malaria, leishmaniasis, toxoplasmosis, or African sleeping sickness, making this protein an attractive reagent for diagnosis of Chagas disease.

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Section: Discussionmentioning

confidence: 99%

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

et al. 2017

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“…All sera were filtered through a membrane (0.45-,um pore size) and then stored at -20°C until used. The control population consisted of 12 children, 6 with acute toxoplasmosis and 6 with cutaneous leishmaniasis, and 20 healthy individuals, none of them with detectable antibodies against T. cruzi (titers, <1:8 by DA-2ME and IHA tests). Urine and serum specimens were collected from this group and used as controls for the ELISAs.…”

Section: Methodsmentioning

confidence: 99%

“…Araujo (2) was not able to detect SAg by ELISA in urine collected from infected mice. A report by Bongertz et al (6) has demonstrated that urine from acutely infected mice and dogs contained antigen detectable by a double diffusion test. We have recently communicated the first demonstration of SAg in human urine samples (10).…”

mentioning

confidence: 99%

Antigenuria in infants with acute and congenital Chagas' disease

Freilij¹,

Corral²,

Katzin³

et al. 1987

J Clin Microbiol

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Detection and partial characterization of Trypanosoma cruzi soluble antigens (SAg) in urine, as well as demonstration of parasite circulating antigens (CAg) in serum from pediatric patients with acute (10 patients) and congenital (10 patients) Chagas' disease, are reported. Classical techniques for parasite detection and antibody serology were also conducted in both groups. Samples collected before the onset of parasiticidal drug treatment were tested by an enzyme-linked immunosorbent assay for SAg and CAg demonstration. The control population consisted of 6 children with acute toxoplasmosis, 6 with cutaneous leishmaniasis, and 20 healthy individuals. Patients with acute cases were 100% positive for both SAg and CAg, whereas patients with congenital disease were 80% CAg positive and 100% SAg positive. Controls yielded negative results in all cases. Partial characterization of SAg from two patients with acute disease was performed by iodination, affinity chromatography, immunoprecipitation, and two-dimensional gel electrophoresis. Two different antigenic glycoproteins (80 kilodaltons, pI 6 to 6.5 and 55 kilodaltons, pI 6.5 to 7) were identified by these methods. Traditional serology and classical parasitologic tests failed, each in a different way, to provide an accurate diagnosis in the total of our patients. The enzyme-linked immunosorbent assay for SAg detection proved to be the most effective procedure for achieving early and precise proof of infection in acute and congenital cases of Chagas' disease.

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“…Increasing attention has also been directed toward demonstration of soluble trypanosome antigens in the urine of infected hosts. Bongertz et al (7) have detected T. cruzi antigens eliminated in the urine of acutely infected mice and dogs. We have reported the occurrence of antigenuria in infants with acute and congenital Chagas' disease (17).…”

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confidence: 99%

Purification and characterization of an 80-kilodalton Trypanosoma cruzi urinary antigen

et al. 1989

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A Trypanosoma cruzi antigen eliminated in the urine of experimentally infected dogs was detected by enzyme-linked immunosorbent assay between 9 and 28 days after infection. The parasite urinary antigen (UAg) was purified by affinity chromatography with polyclonal antibodies to T. cruzi. The eluate of the antibody column was subjected to high-performance liquid chromatography and showed a single peak of A280. This antigen was the only parasite component found in the urine of infected dogs during the course of acute T. cruzi infection. Antigen characterization was performed by two-dimensional gel electrophoresis, lectin affinity chromatography, proteolytic digestion, and Western blotting (immunoblotting). The isolated UAg exhibited a relative molecular size of 80 kilodaltons (kDa), an isoelectric point of 6.2 to 6.8, binding to concanavalin A, and sensitivity to trypsin. The parasite antigen was electroeluted from polyacrylamide gels and subjected to acid hydrolysis and amino acid analysis by reverse-phase high-performance liquid chromatography. The 80-kDa glycoprotein was recognized by serum antibodies from a wide variety of T. cruzi-infected hosts. The UAg proved to be a highly antigenic component present in different strains of T. cruzi. This 80-kDa polypeptide resembles one of the parasite antigens previously found in the urine of patients with acute Chagas' disease.

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Trypanosoma cruzi: circulating antigens

Cited by 8 publications

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Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

Antigenuria in infants with acute and congenital Chagas' disease

Purification and characterization of an 80-kilodalton Trypanosoma cruzi urinary antigen

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