Metodos usados em microanatomia e histologia entomológica

Barth, Rudolf

doi:10.1590/s0074-02761958000200005

Cited by 7 publications

(3 citation statements)

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“…For the histological analyses, we used 10 fourth instar larvae that were fixed in Duboscq solution for insects: 150 mL of ethanol 80%, 60 mL of formaldehyde, 15 mL of glacial acetic acid and 1 g of picric acid (Barth, ) in an incubator at 36°C during 24 hr; then dehydrated with increasing concentrations of ethanol (70, 80, 90, and 95%) during 30 min each. The samples were infiltrated in Leica historesin during 72 hr, and then polymerized in the same historesin, and 3 μm sections were obtained using a LEICA RM2235 microtome.…”

Section: Methodsmentioning

confidence: 99%

Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring

Campeón‐Morales

Richardi

Rivera‐Páez

2019

Microscopy Res & Technique

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Chironomids have been widely used in environmental monitoring and toxicity assays. Assessment criteria for chironomids range from the molecular‐biochemical level to the population level. However, the use of markers to evaluate cellular and anatomical changes in organs and systems of individuals under contaminant exposure is still incipient. In this study, we conducted a histological description of the main systems and organs of immature Chironomus columbiensis. We used fourth instar larvae, obtained from a standardized culture, and confirmed this state through morphological and molecular methods. Larvae were fixed in Duboscq solution for insects during 48 hr, dehydrated in increasing dilutions of ethanol, and embedded and mounted in historesin to obtain 3 μm sections. The digestive, nervous, excretory, and integumentary systems and the fat body and testicles were analyzed through longitudinal and transversal sections, stained with hematoxylin–eosin (HE), and further photographed and described through light microscopy. This is the first description of internal morphology performed for C. columbiensis and can help in future histopathological analysis, because through bioaccumulation some of these organs are contaminant targets. It can even be a great taxonomic tool, since the characteristics of the histological pattern of C. columbiensis presented differences compared to other descriptions made in chironomids.

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Section: Methodsmentioning

confidence: 99%

Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring

Campeón‐Morales

Richardi

Rivera‐Páez

2019

Microscopy Res & Technique

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“…Os aleirodídeos, coletados sobre folhas transportadas para laboratório, foram montados em lâminas conforme preconizado por Costa Lima (1942b), Baker & Wharton (1952) e Barth (1958). No primeiro método, pupários foram colocados em cápsula de porcelana com KOH a 10% e aquecidos em banhomaria até a fervura.…”

Section: Methodsunclassified

“…A metodologia de Barth (1958) foi utilizada, para o material de coloração escura, que era a maior parte. O material foi tratado inicialmente por solução de álcool (600GL a 900GL), enxugado com papel de filtro, tratando-se em seguida com diafanol (10 partes de dióxido de cloro e uma parte de ácido nítrico oficinal), mantendo no escuro até a destruição completa dos pigmentos.…”

Section: Methodsunclassified

Aleirodídeos (Homoptera: Aleyrodidae) em plantas cítricas no Brasil: distribuição e identificação

Cassino

Nascimento²

1999

An. Soc. Entomol. Bras.

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An. Soc. Entomol. Brasil 28(1): 75-83 (1999) White Fly (Homoptera: Aleyrodidae) on Citrus Trees in Brazil:Distribution and ldentification ABSTRACT-The geographical distribution of six species of white fly associated to Citrus spp. in Brazil is presented. Geographica1 distribution, hosts and natural enemies were based on material from 13 Brazilian States, field monitoring and visits to the more States and also review of the literature. Identification key was based on genera1 characters, morphologycal and macroscopycal of the "puparium".KEY WORDS: Insecta, natural enemies.RESUMO -É apresentada a distribuição de seis espécies de aleirodídeos mais comuns na citricultura brasileira. A distribuição geográfica, hospedeiros e inimigos naturais destas espécies, baseou-se em materiais recebidos de 13 estados, monitoramento e visitas técnicas em mais três estados e revisão da literatura. A chave de identificação das espécies baseou-se em características gerais, morfológicas e macroscópicas dos "pupários". PALAVRAS-CHAVE: Insecta, inimigos naturais.Os aleirodídeos estão amplamente distribuídos geograficamente e vivem sobre um grande número de plantas silvestres e ornamentais. Entretanto, diversas espécies tem sido apontadas como pragas de culturas de importância econômica, por simplesmente sugarem a seiva das plantas, outras por transmitirem viroses e substâncias toxicogênicas, por facilitarem o ataque de patógenos ou proporcionarem o aparecimento de fumagina sobre seus dejetos.Em estudos sobre "moscas brancas", nome vulgar das espécies de Aleyrodidae, subordem Sternorrhyncha como vetores de vírus, Costa (1969) informa que Aleurothixus floccosus (Maskell) foi relacionado com a transmissão da "tristeza dos citros", sendo, entretanto, esse registro duvidoso. Algumas espécies estão associadas a Cítrus spp. e, no Brasil, das contribuições que tratam de aleirodídeos em plantas cítricas, destacam-se: Ihering (1897); Hempel (1899Hempel ( , 1904Hempel ( , 1922a; Bondar

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Nervous system of Triatoma infestans

Insausti

1994

Journal of Morphology

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The anatomy of the adult nervous system of the haematophagous bug Triatoma infestans has been studied by means of dissections and histology. The central nervous system comprises three nervous masses: the brain+suboesophageal ganglion, the prothoracic ganglion, and the posterior fused ganglion (meso+metathoracic+abdominal ganglia). The form of the brain is determined by the tubular head and the highly developed muscles of the pharyngeal pump. The prothoracic ganglion is located near the posternum, the posterior ganglionic mass near the mesosternum. A significative variation of the branching pattern of abdominal nerves is reported. The innervations of mouth parts, salivary glands, muscles, retrocerebral complex, spiracles, rectum, reproductive organs, alary muscles, and peripheral nerves are described.

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Metodos usados em microanatomia e histologia entomológica

Cited by 7 publications

References 0 publications

Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring

Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring

Aleirodídeos (Homoptera: Aleyrodidae) em plantas cítricas no Brasil: distribuição e identificação

Nervous system of Triatoma infestans

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