Sôbre u'a modificação do meio de Monteverde

Abstract

<abstract language="eng">It is well known that the culture media used in the presumptive diagnosis of suspiciuous colonies from plates inoculated with stools for isolation of enteric organisms do not always correctly indicate the major groups of enterobacteria. In an effort to obtain a medium affording more exact indications, several media (1-9) have been tested. Modifications of some of these media have also been tested with the result that a satisfactory modification of Monteverde's medium was finaly selected. This proved to be most satisfactory, affording, as a result of only one inoculation, a complete series of basic indications. The modification involves changes in the formula, in the method of preparation and in the manner of storage. The formulae are: A. Thymol blue indicator: NaOH 0.1/N .............. 34.4 ml; Thymol blue .............. 1.6 g; Water .................... 65.6 ml. B. Andrade's indicator. C. Urea and sugar solution: Urea ..................... 20 g; Lactose ................... 30 g; Sucrose ................... 30 g; Water .................... 100 ml. The mixture (C.) should be warmed slightly in order to dissolve the ingredients rapidly. Sterilise by filtration (Seitz). Keep stock in refrigeratior. The modification of Monteverde's medium is prepared in two parts. Semi-solid part - Peptone (Difco) 2.0 g; NaCl 0.5 g; Agar 0.5 g; Water 100.0 ml. Boil to dissolve the ingredients. Adjust pH with NaOH to 7.3-7.4. Boil again for precipitation. Filter through cotton. Ad indicators "A" 0.3 ml and "B" 1.0 ml. Sterilise in autoclave 115ºC, 15 minutes in amounts not higher than 200 ml. Just before using, add solution "C" asseptically in amounts of 10 ml to 200 ml of the melted semi-solid medium, maintained at 48-50ºC. Solid part - Peptone (Difco) 1.5 g; Trypticase (BBL) 0.5 g; Agar 2.0 g; Water 100,00 ml. Boil to dissolve the ingredients. Adjust pH with NaOH to 7.3-7.4. Boils again. Filter through cotton. Add indicators "A" 0.3 ml and "B" 1.0 ml; ferrous ammonium sulfate 0.02 g; sodiun thiosulfate 0.02 g. Sterilise in autoclave 115ºC, 15 minutes in amounts not higher than 200 ml. Just before using, add solution "C" asseptically in amounts of 10 ml to 200 ml of the melted solid medium, maintained at 48-50ºC. Final medium - The semi-solid part is dispensed first (tubes about 12 x 120 mm) in 2.5 ml amounts and left to harden at room temperature, in vertical position. The solid part is dispensed over the hardened semi-solid one in amounts from 2.0 ml to 2.5 ml and left to harden in slant position, affording a butt of 12 to 15 mm. The tubes of medium should be subjected to a sterility test in the incubator, overnight. Tubes showing spontaneous gas bubbles (air) should then be discarded. The medium should be stored in the incubator (37ºC), for not more than 2 to 4 days. Storage of the tubes in the ice-box produces the absorption of air which is released as bubbles when the tubes are incubated at 37ºC after inoculation. This fact confirmed the observation of ARCHAMBAULT & McCRADY (10) who worked with liquid me...

Incidence and antimicrobial resistance of enteropathogens isolated from an integrated aquaculture system

“…The enrichment media used for all samples were alkaline peptone water (APW) (Oxoid), tetrathionate broth (Oxoid) (BAM ⁄ FDA 2003), Silliker's broth (Silliker et al 1964) incubated at 37 ± 0AE2°C and Rappaport-Vassiliadis medium (Oxoid) at 42 ± 0AE2°C. After 24 h, to detect Salmonella and Shigella, the cultures were streaked onto Hektoen enteric agar (Oxoid) and xylose lysine desoxycholate agar (Oxoid) incubated at 37°C for 24 h, then suspected colonies were subjected to presumptive identification into Costa and Vernin agar (Costa and Vernin 1955). For the detection of Vibrio, APW incubated at 37°C for 8 h was streaked onto TCBS agar (Oxoid) and after 24 h onto glutamate starch phenol red agar (Merck, Darmstadt, Germany) for Aeromonas detection.…”

Contribuição para o conhecimento do vírus do mosaico do quenopódio

“…SLQ.li�• , J:!�F.IJÇ,Ã_Q, J:1:J�A O CONHECIMENTO DO VÍRUS 12..Q MOSAICO DO Q_UENOPÓDIO Estudos relativos aos hospedeiros, à inati vaç�o t�rmica 9 à concentraç�o no suco da planta, à resis t §ncia à desnaturaç5o do V M Q foram realizados poa J.Aº Costa e C.W.Bennett (5).…”